Voluntary intake,digestibility and nitrogen utilization by sheep fed ensiled forage legumes

An experiment was conducted to compare the nutritive value of a range of ensiled forage legumes. Silages were prepared from late second‐cut lotus (Lotus corniculatus), first‐cut sainfoin (Onobrychis viciifolia) and both early and late second‐cut red clover (Trifolium pratense) and lucerne (Medicago sativa). Each experimental silage was offered to six Suffolk‐cross wether lambs, aged 10 months, housed in metabolism crates. Voluntary intakes of dry matter ranged from 71 to 81 g kg^?1 liveweight^0·75 d^?1. Voluntary intakes were similar on the lotus, sainfoin and late‐cut red clover silages, but the voluntary intake on the lotus silage was significantly higher than that on the lucerne silages and early‐cut red clover silage. Digestibility of organic matter in the dry matter was highest for the lotus silage (0·650), and lowest for the sainfoin silage (0·527). Although most of the N in the sainfoin silage appeared to be in an indigestible form, N digestibility was approximately 0·70 for the other legume silages. The highest loss of N in urine, 0·75 of N intake, was recorded for lambs offered the lucerne silage. Differences in N intake, N loss in faeces and N loss in urine led to statistically significant differences in the amount of N retained, with the highest and lowest N balances recorded for the lotus (16 g N d^?1) and sainfoin (?2 g N d^?1) silages respectively. The results confirm that these high protein forages have high intake potential. While low N digestibility appears to limit the nutritional value of sainfoin, further research could formulate feeding strategies that improve the efficiency with which the protein from red clover, lucerne and lotus is utilized.     

Production performance and meat quality of grazing lambs finished on red clover, lucerne or perennial ryegrass swards

The combined benefits of a high crude protein concentration, and possible protein protection and growth‐promoting properties, make forage legumes potentially attractive as a natural means of increasing liveweight gain and time to slaughter of lambs in lamb finishing systems. An experiment was conducted to compare the production performance and meat quality of grazing lambs finished on red clover (Trifolium pratense), lucerne (Medicago sativa) or perennial ryegrass (Lolium perenne) swards. Replicate (n = 2) swards of red clover, lucerne and perennial ryegrass were rotationally grazed by ten ram lambs and ten ewe lambs from weaning until selection for slaughter at UK fat class 3L. Lambs grazing the red clover sward had a significantly higher liveweight gain and required significantly fewer days to slaughter than lambs grazing the lucerne sward (305 g d^?1 vs. 243 g d^?1; 38 d vs. 50 d), which in turn had a higher liveweight gain and required fewer days to slaughter than lambs grazing the perennial ryegrass sward (184 g d^?1; 66 d). Lambs grazing the red clover and lucerne swards had significantly higher herbage intakes than those grazing the perennial ryegrass sward (2·06, 1·72 and 1·16 kg DM d^?1 respectively), but in vivo digestibility of herbage was similar. Lambs grazing the red clover and lucerne swards also had significantly higher serum urea concentrations than those grazing ryegrass (12·5, 11·1 and 6·2 mmol L^?1 respectively). Killing‐out percentage was significantly higher for lambs grazing the red clover sward than for lambs grazing the perennial ryegrass sward (48% vs. 46%). There were no significant effects of finishing system on meat flavour, but meat from lambs finished on the lucerne sward was oxidatively less stable than that from lambs finished on the perennial ryegrass sward. Grazing the forage legume swards significantly increased the proportion of linoleic and linolenic acid in muscle tissue, and therefore the proportion of unsaturated to saturated fatty acids (0·19, 0·16 and 0·12 for the red clover, lucerne and perennial ryegrass swards respectively). However, the n?6/n?3 ratio was significantly lower for the muscle of lambs grazing the perennial ryegrass sward compared with those grazing the forage legume swards (1·13, 1·08 and 0·98 for the red clover, lucerne and perennial ryegrass swards respectively). The results indicate that by grazing lambs on forage legume swards it is possible to increase individual lamb performance without compromising meat quality.     

Intake, liveweight gain and feed preference by steers fed combinations of lucerne and Westerwolds ryegrass silages

Nutritive value and voluntary intake of legumes are generally considered to be higher than those of grasses when ensiled at similar digestibility, although high levels of soluble protein can result in low N utilization by animals and high losses to the environment. The objectives of this experiment were to describe the optimum combination of Westerwolds ryegrass ( Lolium multiflorum Lam. cv. Aubade) and lucerne ( Medicago sativa L. cv. AC Caribou) silages to maximize liveweight gain of steers fed silage, determine chemical components that are important and ascertain whether steers selected the optimum mixture when given a choice. Both silages contained similar concentrations of dry matter (DM), acid-detergent fibre (ADF) and organic acids, but lucerne silage had higher concentrations of N, soluble-N and ammonia-N. Westerwolds ryegrass silage contained more neutral-detergent fibre (NDF). In a 12-week experiment, voluntary intake by Hereford steers was not influenced when the proportion of the two silages was changed from 1 to 0 in 0·25 increments. However, liveweight gain and feed efficiency increased linearly ( P  < 0·001) as the proportion of ryegrass silage fed was increased. When preconditioned to either of the two silages, steers showed a significant preference for ryegrass over lucerne ( P  < 0·05). When conditioned to a mixture of both silages, no preference was elicited. It is suggested that extensive solubilization and deamination of protein in the lucerne silage may have caused the preference for Westerwolds ryegrass silage and the higher liveweight gains on diets containing higher proportions of Westerwolds ryegrass silage.     

Effects of harvest date of timothy (Phleum pratense) on its nutritive value, and on the voluntary silage intake and liveweight gain of lambs

The effects of harvest date of timothy on the chemical composition of herbage and silage, and on the voluntary intake, liveweight gain and feed conversion efficiency by finishing lambs, were evaluated. The herbage was harvested and ensiled on three dates: 16 June (before heading), 20 June and 26 June. The silages were analysed for chemical composition and degradation characteristics by an in vitro gas production (GP) technique with end-point measurements (72 h) of degradability of organic matter (OM) and neutral-detergent fibre (NDF). There were clear effects of later dates of harvest increasing the concentration of NDF, and reducing the degradability of OM and NDF, and the rate of GP, of silages made from this herbage. The silages were fed ad libitum to lambs in a feeding experiment using a Latin square design. Later harvest dates decreased the voluntary intake of silage, liveweight gain and feed conversion efficiency. Lambs fed the early-cut silage had a liveweight gain of 152 g d⁻¹ and those fed the silage harvested 10 d later had a liveweight gain of 76 g d⁻¹. Changes in the chemical composition of herbage and silage and in in vitro degradation characteristics of silages with later harvests were associated, to a large extent, with the reduction in voluntary intake and liveweight gain of lambs.     

The effect of harvest date and inoculation on the yield, fermentation characteristics and feeding value of forage pea and field bean silages

Two experiments describe the ensiling potential of whole‐crop forage peas (Pisum sativum) and field beans (Vicia faba). In Experiment 1, forage peas (cv. Magnus) and field beans (cv. Mayo) were harvested at 10, 12 and 14 weeks after sowing, and ensiled in 10 kg mini‐silos either untreated or treated with an inoculant (Lactobacillus plantarum). In terms of yield and ensiling potential, the optimum growth stage for harvesting forage peas occurred at 12 weeks of growth. In contrast, delaying the harvest of field beans until 14 weeks gave the highest yields of dry matter (DM) and crude protein (CP). Changes in crop maturity had little effect on the chemical composition of the fresh forages, but between‐harvest date differences were observed in the DM, ammonia‐N, CP, water‐soluble carbohydrates (WSC), acid‐detergent fibre (ADF), neutral‐detergent fibre (NDF), lactic acid and volatile fatty acids (VFA) concentrations and pH of the corresponding silages. Fermentation was improved by applying an inoculant. In Experiment 2, forage peas and field beans were harvested at 14 weeks after sowing and ensiled as round‐bale silage, either untreated or treated with an inoculant. The yields of the crops were similar, and the only difference in the chemical composition of the wilted forages was a higher CP concentration in the field beans. However, after the ensiling process was complete, the forage pea silages were found to have significantly higher DM, WSC, starch and butyric acid concentrations compared with the field bean silages, and lower ammonia‐N, CP, ADF, acetic acid and lactic acid concentrations. Inoculation was found to increase the lactic acid concentration and reduce the pH and ammonia‐N and acetic acid concentrations of the silages. Each of the silages produced in Experiment 2 was offered to six Suffolk crossbred wether lambs, aged 10 months. Voluntary DM intakes were similar on all treatments, despite the apparent digestibility of the forage pea silages being significantly higher than that of the field bean silages. Nitrogen retention was higher for lambs offered forage pea silage. Application of an inoculant was found to have a negative effect on the amount of N retained, indicating the necessity for more detailed investigations into proteolytic activity within these crops during the fermentation process.     

Yield,nutritive value and silage fermentation of kura clover‐reed canarygrass and lucerne herbages in northern USA

Excellent winter hardiness, persistence and nutritive value of both kura clover (Trifolium ambiguum M. Bieb.) and reed canarygrass (Phalaris arundinacea L.) suggest that intercropping these species could substitute for lucerne (Medicago sativa L.). The dry matter (DM) yield and nutritive value of herbage, and silage characteristics of kura clover‐reed canarygrass (KC‐RCG) herbage, were compared to those of lucerne over two growth cycles near Arlington, WI, USA. First and second growths of lucerne and KC‐RCG herbage were sampled four times at 1‐week intervals and ensiled for 100 d. Yield of DM of the KC‐RCG was 0·23–0·57 greater than that of lucerne on sampling dates in the first growth cycle, with no differences in DM yield in the second growth cycle. The pH of lucerne silage was lower than that of KC‐RCG silage in the first growth, and the opposite occurred in second growth, which was attributed to maturity differences and the proportion of kura clover in the mixture. Lactate concentration was lower in KC‐RCG than lucerne silages in both growth cycles. The lucerne and KC‐RCG silages had similar in vitro DM digestibility except for the final sampling date in the first growth cycle when neutral‐detergent fibre concentration of KC‐RCG herbage exceeded 550 g kg^?1 DM. Crude protein concentration was greater in lucerne silage than in KC‐RCG silage in both growth cycles. Overall, differences in nutritive value and silage fermentation between the two herbages were minimal across growth cycles. These results suggest that a KC‐RCG sward is a viable alternative to lucerne in northern environments of the USA where lucerne production may be limited by winter injury or edaphic factors.     

Mixing whole-crop pea–oat silage and grass–clover silage: positive effects on intake and milk production of dairy cows

Forty‐eight high‐yielding dairy cows of the Swedish Red breed were used to examine the effects of providing pea–oat silage (P), grass–clover silage (G) and a 0·50:0·50 mixture of the silages (M) ad libitum in diets with two concentrate levels (7 or 10 kg d^?1). A 9‐week experiment, including a 2‐week pre‐experimental period in which the cows were all fed the same diet, and an in vivo apparent digestibility study were conducted comparing the six dietary treatments (M7, M10, P7, P10, G7, G10). Intake and digestibility of the diets and milk production and live weight of the cows were measured. The G silage [11·3 MJ ME kg^?1 dry matter (DM)] was first‐cut grass herbage wilted for 24 h prior to addition of an additive, containing formic acid, propionic acid and ammonia, at 4 L t^?1 fresh matter (FM). The P forage was cut when the peas were at pod fill and ensiled directly with 6 L t^?1 FM of the same additive. The main hypothesis tested, that cows fed the M silage would produce more milk than the cows fed either the P or the G silages, was confirmed. The cows fed the M7 dietary treatment had similar milk yield and milk composition to cows offered the M10, G10 and P10 dietary treatments, and cows offered the G7 and P7 dietary treatments had lower milk and milk protein yields. This suggested that a mixed ration of pea–oat bi‐crop and grass–clover silage has a concentrate‐sparing effect, and that the use of pea–oat bi‐crop and grass–clover silage as a mixed ration for high‐yielding dairy cows can be recommended.     

Grass silage as a basic feed for store lambs. 1. Effect of wilting, chop length and stage of maturity of grass silage on intake and performance of store lambs

Two experiments were carried out with grass silages cut at a leafy (Experiment 1) and a more mature (Experiment 2) stage of growth to evaluate the effect of wilting and chop length on silage intake and performance of store lambs. In each experiment, the herbage was cut with a rotary mower and was either ensiled within 24 h as unwilled silage (U) or wilted for 1–3 d (W). Each silage type was harvested with either a double-chop harvester (D) or a precision-chop harvester (P). All silages were treated with formic acid at 3 1 t^?1 and were well preserved. The silages were fed ad libitum to Suffolk crossbred store lambs (twenty-four lambs per treatment) without any supplement over a period of 8 or 9 weeks. Wilting of the silages had little effect on silage intake (797 vs. 809g dry matter (DM) d^?1) or on lamb performance in Experiment 1. In Experiment 2, wilting of the D silage increased silage DM intake (589 vs. 534 g DM d^?1; +10%) and reduced the extent of liveweight losses. Wilting of the P silage reduced silage intake (770 vs. 791g DM d^?1; -3%) and reduced liveweight gains. In Experiment 1 intakes of the D silages were 650–667g DM d^?1 and just maintained lamb live weights. Intakes of the P silages were 39–49% higher than the D silages (927–968 g DM d^?1) and increased liveweight gains. In Experiment 2 intakes of the D silages were 534–589 g DM d^?1 and resulted in a loss in lamb live weight. Precision-chopping increased silage intakes by 31–48% (770–791 DM d^?1)in Experiment 2 and improved lamb liveweight gains. Lamb performance was higher on the UP silage than on the WP silage. The rumen retention lime (RRT), estimated from the rumen contents of the lambs at slaughter and their silage intake before slaughter, was much shorter for the lambs fed on the P silages (12.6–20.6 h) than those fed on the D silages (21.4–29.3 h) in each experiment. Silage intake and liveweight gain were positively related to silage in vivo DM digestibility (DMD), whereas RRT was negatively related to DMD. However, there were distinct differences between the P and D silages in the elevation and, to a lesser extent, in the slope of the regression lines, indicating that intake of D silage was limited by factors other than the digestibility of the silage The results of this study show that the chop length of grass silage had a far greater effect on intake and on lamb performance than silage digestibility, whereas wilting had little or no effect.     

Characteristics of baled silage made from first and second harvests of wilted and severely wilted forages

First and second harvests of lucerne (Medicago sativa L.), perennial ryegrass (Lolium perenne L.) and a lucerne–perennial ryegrass mixture [80 or 144 g kg^?1 dry matter (DM) of ryegrass] at the first and second harvests were cut and conditioned, wilted to 500 or 700 g DM kg^?1 then baled and stretch‐wrapped for silage on the same dates. Lucerne bales were denser (411 kg m^?3) than bales of perennial ryegrass (331 kg m^?3) (P < 0·05). After an 8‐month storage period, silage made from high DM‐content forage had a higher concentration of neutral‐detergent fibre (NDF) and was less digestible than that made from low DM‐content forage. Daily DM intakes by beef steers, when the silages of the second harvest were fed ad libitum, were 31·2, 31·2 and 22·3 g kg^?1 live weight for lucerne, lucerne–perennial ryegrass mixture and perennial ryegrass silages, respectively (P < 0·01), when the herbage had been wilted to 500 g kg^?1. In vivo digestibility of NDF in the lucerne–perennial ryegrass mixture silage (0·587) was significantly lower than that of perennial ryegrass silage (0·763) but higher than lucerne silage (0·518). Higher intakes of baled lucerne silage tended to offset its lower digestibility values. Lucerne–perennial ryegrass mixture silage had a higher DM and NDF digestibility than lucerne silage, indicating perhaps the presence of associative effects.     

Effects of feeding clover silages on feed intake, milk production and digestion in dairy cows

Silages were made from pure crops of perennial ryegrass, red clover and white clover over 2 years. In all but one case the silage was stored as bales. A silage additive specially adapted for bales (Kofasil Ultra^TM) was used for all silages and they were all of good hygienic quality. The additive contained sodium nitrite, hexamethylene, tetraamine sodium bensoate and sodium propionate. The silages were offered ad libitum, either pure or mixed [grass/clover 0·50/0·50 on a dry‐matter (DM) basis] with a fixed amount (8 kg) of concentrate. Two experiments, one in each year, were performed with high‐yielding multiparous dairy cows in mid‐lactation, and both rumen‐cannulated and intact cows were used. The experiments were carried out using an incomplete changeover design with fifteen cows and five treatments each year. The cows consumed large quantities of these silages (12·7–16·3 kg DM per cow per day). The highest intakes were obtained when the red clover and the 0·50 red clover:0·50 perennial ryegrass silage diets were offered. However, there was a difference between years. In year 1, 0·50 red clover:0·50 perennial ryegrass and 0·50 white clover:0·50 perennial ryegrass silage diets showed the highest intakes while pure perennial ryegrass and white clover silage diets gave lower intakes. In year 2 the highest intake of silage was obtained when the diet containing silage from red clover from a second cut was offered, while the silage from red clover from a first cut gave the lowest intake. The voluntary intakes of silages from white clover and perennial ryegrass were intermediate. No cases of bloat or other digestive disturbances were observed. Milk yield was significantly lower for the perennial ryegrass silage diet compared with all other diets in year 1. In year 2 milk yield was highest for the white clover silage diets and lowest for the red clover silage diets from both cuts. In year 1 there were relatively small differences in milk composition while in year 2 milk fat content was significantly lower with white clover silage diet and milk protein content was significantly higher with the perennial ryegrass diet. The overall conclusion from these experiments was that cows were able to consume large quantities of pure legume silage without serious disturbance to their metabolism. Differences in measurements of rumen metabolism were found between diets and especially between years. Milk production differences appears to be coupled to both differences in rumen physical characteristics, such as passage rate and particle size as well as differences in volatile fatty acid production in the rumen.     

Effect of formic acid and a bacterial inoculant on the amino acid composition of grass silage and on animal performance

An Italian ryegrass and hybrid ryegrass sward was harvested on 11 May 1994. The mean dry‐matter (DM) content of the herbage was 197 g kg^–1 fresh matter (FM), and mean nitrogen and water‐soluble carbohydrate contents were 20 and 272 g kg^–1 DM respectively. Approximately 72% of total nitrogen (TN) was in the form of protein‐nitrogen. The herbage was treated with either no additive, formic acid (3·3 l t^–1) (Add‐F, BP) or inoculant (2·3 l t^–1) (Live‐system, Genus) and ensiled in 100 t silos. Changes in effluent composition with time showed that silage fermentation and protein breakdown were delayed by treatment with formic acid. Formic acid and inoculant treatments also inhibited amino acid catabolism during ensilage. All silages were well fermented at opening with pH values < 4·0 and ammonia‐N concentrations of ≤ 50 g kg^–1 TN after 120 d ensilage. Treatment had an effect on protein breakdown as measured by free amino acid concentration, with values of 21·5, 18·2 and 13·2 mol kg^–1 N at opening (191 d) for untreated, formic acid‐treated and inoculated silages respectively. Amino acid catabolism occurred to the greatest extent in untreated silages with significant decreases in glutamic acid, lysine and arginine, and increases in gamma amino butyric acid and ornithine. The silages were offered ad libitum without concentrate supplementation to thirty‐six Charolais beef steers for a period of 69 d (mean live weight 401 kg). Silage dry‐matter intakes and liveweight gains were significantly (P < 0·05) higher on the treated silages. Silage dry‐matter intakes were 7·42, 8·41 and 8·23 kg d^–1 (s.e.d. 0·27) with liveweight gains of 0·66, 0·94 and 0·89 kg d^–1 (s.e.d. 0·058) for untreated, formic acid‐treated and inoculated silage‐fed cattle respectively. In conclusion, additives increased the intake of silage and liveweight gain by the beef steers, and it is suggested that this may be caused in part by the amino acid balance in these silages.     

Nutritive value of barley/kale bi-crop silage for lactating dairy cows

Six mid‐lactation multiparous Holstein–Friesian dairy cows were used to examine the potential of a fermented whole‐crop barley (Hordeum vulgare)/kale (Brassica oleracea) bi‐crop as a feed compared with a first‐cut perennial ryegrass silage. The barley/kale bi‐crop was grown as a strip intercrop, and was harvested and ensiled as an intimate mixture [0·80 barley and 0·20 kale on a dry‐matter (DM) basis]. Animals were offered ad libitum access to one of three experimental diets in a duplicated Latin Square design experiment: (i) Bi‐crop (the barley/kale bi‐crop); (ii) Grass (the grass silage); and (iii) Mix (a 1:1 fresh mixture of Bi‐crop and Grass). All animals also received a standard dairy concentrate at a rate of 4 kg d^?1 in equal portions at each of two milkings. The Bi‐crop and Grass silages contained 346 and 293 g DM kg^?1, 108 and 168 g crude protein kg^?1 DM, 268 and 36 g starch kg^?1 DM, and had pH values of 3·87 and 3·80 respectively. Animals offered the two bi‐crop silage‐containing diets consumed more forage DM than those offered grass silage (14·6, 14·9 and 12·6 kg DM d^?1 for Bi‐crop, Mix and Grass respectively; s.e.d. 0·45, P < 0·01) and yielded more milk (24·0, 23·9, 22·6 kg d^?1 for Bi‐crop, Mix and Grass respectively; s.e.d. 0·26, P < 0·01). However, differences in the partitioning of dietary nitrogen towards milk protein and away from excretion in urine suggest a more efficient (rumen) utilization of feed protein by animals offered diets containing the bi‐crop silage. It is concluded that, despite having a low crude protein concentration, barley/kale bi‐crop silage offers excellent potential as a feed for lactating dairy cows.     

Effect of tannin extracts on protein degradation during ensiling of ryegrass or lucerne

Eleven laboratory‐scale trials were undertaken in different years where ryegrass (Lolium perenne L.) or lucerne (Medicago sativa L.) were ensiled with different concentrations of tannin extracts (quebracho, Schinopsis balansae Engl., mimosa, Acacia mearnsii DE WILD.), and the effects on protein degradation were assessed. The dry‐matter (DM) content in grass silages ranged between 186 and 469 g/kg and in lucerne silages between 187 and 503 g/kg. Tannin extract, either quebracho or mimosa, was applied at 0–30 g/kg forage DM. Commercial additives such as Lactobacillus plantarum, formic acid or hexamine + NaNO₂ were applied in two of the grass trials and in six of the lucerne trials. Eight of the trials incorporated a maximum ensiling duration of 90 or 180 days in addition to replicates which were opened and evaluated at earlier stages. All trials included silages which were assessed after at least 49 days of anaerobic storage. The crude protein (CP) fraction A (non‐protein nitrogen, NPN) as proportion of total CP, served as the main indicator for proteolysis. In ryegrass, in general, the level of proteolysis was lower than in lucerne. A correlation of DM content in silages and degree of proteolysis was only evident for ryegrass. In both forages, the degradation of true protein slowed considerably after 24 days of ensiling. True protein was conserved most with the highest level of tannin extract addition. However, in lucerne, the combination of formate with lactobacilli was equally effective up to 330 g DM/kg, and deamination was further inhibited by formic acid compared to tannin extracts.     

Comparison of the performance of dairy cows offered kale,swedes and perennial ryegrass herbage in situ and perennial ryegrass silage fed indoors in late pregnancy during winter in Ireland

The effects on the performance of dairy cows offered kale, swedes, and perennial ryegrass in situ and perennial ryegrass silage fed indoors to dairy cows pre‐partum during winter in Ireland was examined. Eighty‐eight spring‐calving dairy cows were randomly assigned to one of four offered treatments; (i) 8 kg of dry matter (DM) of kale leaf and stem + 4 kg DM of perennial ryegrass silage (treatment K), (ii) 8 kg DM of the root and leaf of swedes + 4 kg DM of perennial ryegrass silage (treatment S), (iii) 12 kg DM of perennial ryegrass herbage (treatment G) offered in situ and (iv) perennial ryegrass silage offered ad libitum indoors (treatment ID). Cows on treatments K, S and ID had a greater (P < 0·001) increase in body condition score (0·20, 0·14 and 0·50 units respectively) pre‐partum than cows on treatment G which lost 0·22 units. Pre‐partum treatment had no effect on variables of milk production in the following lactation. In the first 100 d of lactation, cows on treatment G pre‐partum had a lower milk fat concentration (35·6 g kg^?1) compared with cows on treatments S and ID (38·3 and 39·3 g kg^?1 respectively). There was no effect of treatment on the intervals between parturition and first insemination (mean 74·6 d) and conception (mean 96·1 d). The results suggest that offering kale and swedes to dairy cows pre‐partum resulted in a similar lactation performance to dairy cows grazing a perennial ryegrass sward or offered perennial ryegrass silage indoors.     

The effect of additives on the ensilage of forage of differing perennial ryegrass and white clover content

Pure perennial ryegrass or perennial ryegrass/white clover mixtures (70:30 and 40:60 on a fresh-matter basis) were ensiled in laboratory silos either untreated or alter treatment with freshly cultured Lactobacillus (Lb.) plantarun or freshly cultured Lb. plantarum plus Lactococcus (Lc.) lactis. freeze-dried Lb. plantarum or freeze-dried Lb. Plantarum plus sodium formate, sodium formate or formic acid. The effect of these additives on silage fermentation characteristics and quality of the resultant silages was examined. There were significant interactions between treatments and herbages for all silage quality parameters measured, except for acetic acid concentration. The influence of additives on the final pH of all silages was small but statistically significant. Lactic acid concentration was not directly related to herbage mixture, overall mean values ranging from 118 to 120 ± 1.5 g kg^?1 dry matter (DM), but wider variation was seen between treatments for individual herbage mixtures. Acetic acid concentrations were significantly (P<0·001) affected by herbage mixture ensiled, increasing linearly as clover content increased from zero to 60%. Untreated control and formic acid-treated silages contained significantly (p<0·001) higher acetic acid concentrations than those treated with other additives. Silage ammonia N concentrations were significantly (p<0 001) influenced by herbage mixture. Lowest ammonia N concentrations (< 50 g kg^?1 DM) were observed in silages that had been treated with formic acid, freshly cultured Lb. plantarum or Lb. plantarum plus Lc. lactis. The fraction 1 leaf protein (FILP) contents of silages were significantly (P <0·001) affected by both treatment and herbage mixture, with consistently and significantly higher values found in freshly cultured inoculant-treated silages. A poor correlation (r²= 0·12) existed between ammonia N and FILP in all silages. The inclusion of up to 60% white clover in the ensiled herbage did not adversely affect final silage quality. However, additive treatment markedly influenced the residual FILP content of silages, those treated with freshly cultured inoculants having the highest values.     

Effects of inoculants and wilting on silage fermentation and nutritive characteristics of red clover–grass mixtures

Various management practices (e.g. wilting, application of silage additives or adding a grass component) can be used to improve silage fermentation of pure red clover (Trifolium pratense L.). Therefore, the aim of this laboratory ensiling study was to investigate the effects of varying proportions of red clover and perennial ryegrass (100/0, 66/33, 33/66, 0/100) on silage quality during two consecutive years. In addition, two wilting levels [target dry matter (DM): 300 vs. 400 g kg^?1] in combination with lactic acid bacteria (LAB) additives were tested. Herbage was ensiled, either untreated or inoculated with homofermentative LAB (low wilted) or homo‐ and heterofermentative LAB (high wilted). In most cases, lactic and acetic acid decreased as the proportions of ryegrass were increased. Data concerning ammonia‐N concentrations showed considerable differences between cuts and years. Silages treated with homofermentative LAB generally had high lactic acid and low final pH, whereas acetic acid and 1,2‐propanediol tended to be higher when homo‐ and heterofermentative LAB were applied. Inoculants had a positive effect on DM losses and ammonia‐N in only a few silages. Wilting decreased DM losses and fermentation acids at most cuts, irrespective of the grass/clover ratio in the herbage mixture. There was a strong year effect on the organic matter digestibility (DOM) of the silages. In conclusion, the optimal strategy for successful silage fermentation of red clover is the ensiling in mixtures with ryegrass. Furthermore, herbage should be wilted to a DM content of about 300–350 g kg^?1. The application of LAB inoculants did not alter the DOM but did improve silage fermentation.     

Silages containing bioactive forage legumes: a promising protein‐rich feed source for growing lambs

Forage legumes that contain secondary compounds are considered to be less susceptible to proteolysis than other legumes, with improved silage quality and possibly improved animal performance. This was investigated feeding five groups of growing lambs for 10 weeks, five silages composed of pure timothy (T; Phleum pratense), mixtures of T with red clover (Trifolium pratense; T‐RC, 50/50 on DM basis), sainfoin (Onobrychis viciifolia; T‐SF), or both (T‐RC‐SF, 50/25/25), or a mixture of the two legumes (RC‐SF). Including SF and/or RC in silages improved silage fermentation as shown by higher lactic acid and lower soluble N and NH₃ contents than in T silage. Voluntary intakes were higher with all the RC‐containing silages than with T and T‐SF. The T‐SF silage had the lowest, and RC‐containing silages the highest average values for daily gain, feed conversion efficiency, live weight and carcass weight. The differing effects of these silages cannot be totally explained by differences in their nutritive value and so may be due to their bioactive compounds impacting differently on feeding motivation and digestive efficiency. These results suggest that including RC in silages is a promising strategy to combine improved animal performance with reduced environmental pressure.     

Grass silage as a basic feed for store lambs. 3. Effect of barley supplementation of silages varying in chop length on silage intake and lamb performance

An experiment was carried out to study the effect of silage chop length and barley supplementation on silage intake and the performance of store lambs. The silages were cut from a perennial ryegrass regrowth at a relatively mature stage of growth in early July. Different types of harvester were used to produce a long silage (L), single-chopped (S), double-chopped (D), medium precision-chopped (MP) or a short precision-chopped (SP) silage. The chop length of the silages averaged 37.4, 12.4, 8.4, 7.0 and 2.9 cm respectively. All silages were treated with formic acid at 2.51 t^?1grass. They were well preserved and of a good quality with a dry-matter (DM) digestibility of 720–760 g kg^?1 DM. The silages were fed ad libitum to Suffolk crossbred store lambs over a period of 10 weeks. Each silage was fed either alone or was supplemented with whole barley at 400 g lamb^?1 d^?1. When offered silage alone, intakes were 738, 679, 773, 980 and 910 (± 30) g DM d^?1 for silages L, S, D, MP and SP respectively. Similar relative differences in intakes between the silages were evident when supplemented with barley. Liveweight gain on silage alone was higher on the precision-chopped silages (85–93 g d^?1) than the long or flail-chopped silages (28–49 g d^?1). Liveweight gain on the precision-chopped silages was also better when supplemented with barley (150 vs. 90–112 (± 90) g d^?1). Silage intake and lamb performance were similar for the MP and SP silages, despite the difference in chop length. Barley supplementation reduced silage intake to a similar extent for all silages (-9%) and increased total DM intake (+ 30%) and metabolizable energy intake (+ 37%). The substitution rates of silage for barley were similar for all silages and averaged 0.24 g silage DM g^?l barley DM. The response in weight gain per 100g of barley fed was similar for all silages and averaged 20.0 g liveweight gain and 13.4 g carcass gain. Feed conversion efficiency of the total diet was similar for the Mail-chopped and precision-chopped silages but was less for the long silage. The results of this study show that the benefit in weight gain due to the higher intake of precision-chopped silage was maintained when the silage was supplemented with a moderate level of barley. Such silage required supplementation with barley (400 g d^?l) to achieve a rate of liveweight gain of 150g d^?1.     

Grass silage as a basic feed for store lambs. 2. Effect of harvesting system and chop length of grass silage on silage intake and performance of store lambs

A perennial ryegrass sward was cut at a leafy stage of growth and harvested with different harvesters to produce silages differing in chop length to evaluate the effect of silage chop length on silage intake and on the performance of store Iambs when silage was fed as the sole diet. The silage was harvested in late May either as long silage (L), single-chopped (S). double-chopped (D). long precision-chopped (LP) or medium precision-chopped (MP) silage with (he appropriate machinery. The silages were treated with formic acid at 2.5 1 t^?1. were well preserved (pH 3.7–3.8) and were of high dry-matter digestibility [749–810 g DMD kg^?1 dry-matter (DM)]. The silages were fed ad libitum as the sole diet to Suffolk crossbred store lambs over a period of 11 weeks. Silage intake and lamb performance progressively increased as silage chop length declined from 32.4 cm (L) to 6.8 cm (MP). Silage intakes were 572, 661, 750, 893 and 1129 (± 21) g DM d^?1 for silages L, S, D, LP and MP respectively. The corresponding daily liveweight gains were -3, 40, 53, 85 and 151 (± 7.6) g d^?l. Similar increases in empty body weight gain and carcass weight gain were obtained as silage chop length declined. Rumen retention time (RRT), estimated from the rumen contents of the lambs at slaughter and their silage intake in the week before slaughter, was much shorter for silages LP and MP compared with silages L or S. Silage intake was negatively related to RRT(b= -24.5 ± 6.1 gDM h^?l RRT). The results of this study showed that high intakes of grass silage and liveweight gains were achieved when grass was cut at a leafy stage of growth and harvested with a precision-chop harvester set to produce a moderate chop length (7 cm). The feeding of long or flail-chopped silages resulted in lower intakes and lower liveweight gains.     

Mixtures of clovers with plantain and chicory improve lamb production performance compared to a ryegrass–white clover sward in the late spring and early summer period

A research programme was undertaken over two consecutive years with the purpose of studying the effect of herb–clover swards on lamb production performance year‐round. The focus of this study was on two consecutive late spring and early summer periods (2011, 2012). In each year, three sward treatments were compared on grazed paddocks with 40 lambs ha^?1: (i) grass–clover mixture (perennial ryegrass [Lolium perenne L.] and white clover [Trifolium repens]); (ii) plantain–clover mixture (plantain [Plantago lanceolata], white clover and red clover [Trifolium pratense]); and (iii) chicory–plantain–clover mixture (plantain, chicory [Cichorium intybus L.] and white and red clovers). Lambs were weighed at 2‐week intervals, and carcass weights and GR tissue depth measurements were obtained at slaughter. In both years, lambs on treatments (ii) and (iii) had greater (P < 0·05) final live weight, liveweight gain, carcass weight, dressing‐out percentage and GR tissue depth measurements, and lower feed conversion ratio compared to lambs on treatment (i). Lamb production was similar in treatments (ii) and (iii) (P > 0·05) in each year. Therefore, during the late spring and early summer period, herb–clover mixture swards were found to be a superior option to perennial ryegrass–white clover for finishing lambs.