伪钝绥螨对二斑叶螨的捕食作用

室内条件下研究了伪钝绥螨对二斑叶螨的捕食作用。结果表明：伪钝绥螨雌成螨对二斑叶螨卵、幼螨、若螨和雌成螨的功能反应均符合HollingⅡ型方程,搜寻效率a′与处理时间Th值分别为0.5902、1.0025、0.9697、0.3146和0.0150、0.0240、0.0520、0.1681。伪钝绥螨的雌成螨喜食二斑叶螨的卵、幼螨和若螨,而对二斑叶螨雌成螨的捕食能力较弱。伪钝绥螨捕食率随着自身密度的增加而下降。捕食者密度低时,捕食率下降较快;随着捕食者密度进一步增加,捕食率下降缓慢,干扰反应方程拟合为E=0.3101P~（-0.412）。     

巴氏钝绥螨防控柑橘全爪螨应用效果

2012～2013年在重庆市的柑橘园内开展了释放巴氏钝绥螨防治柑橘全爪螨应用示范.示范结果显示,每年4月下旬释放巴氏钝绥螨,柑橘全爪螨数量一直被控制在防治指标以下;对巴氏钝绥螨越冬情况调查结果表明,巴氏钝绥螨能够在重庆万州顺利越冬.     

提高花粉饲养伪钝绥螨繁殖力的试验

单独用苹果花粉喂养伪钝绥螨,其后代生活力降低。本文报道在苹果花粉中分别添加30％王浆水、30％蜂蜜水饲养伪钝绥螨,或用苹果花粉和朱砂叶螨交替喂食。结果表明,其后代成螨性比较单独用苹果花粉喂食者均有提高,雌成螨自食其卵现象基本消除。其中以苹果花粉和朱砂叶螨交替供食者效果最好,后代成活率明显提高。以苹果花粉为主食料,定期添加朱砂叶螨,5周后伪钝绥螨的增殖倍数与完全取食朱砂叶螨者相近。     

温度对伪钝绥螨实验种群的影响

本文研究了5个不同恒温条件下伪钝绥螨取食二斑叶螨时的发育和繁殖情况,并对该实验种群的生命参数进行了分析。结果表明,在19~31℃范围内,伪钝绥螨各螨态和整个未成熟期的发育历期随温度的升高而缩短,发育速率随温度的升高而加快;运用直线回归法计算出了伪钝绥螨各螨态的发育起点温度及有效积温。伪钝绥螨产卵期在22℃时最长,在31℃时最短;日均卵量随温度的升高而增大。伪钝绥螨净增殖率Ro在28℃时最高(60.256),在19℃时最低(4.174);内禀增长率rm和周限增长率λ皆是在31℃时最高,在19℃时最低;31℃时种群加倍时间t最短(2.79 d),19℃时最长(15.868 d)。     

山楂叶螨对胡瓜钝绥螨的吸引作用研究

采用两侧吸引作用试验测定天敌胡瓜钝绥螨Amblyseius cucumeris Oudemans在田间对山楂叶螨的搜索和控制能力。结果表明:山楂叶螨不同螨态对胡瓜钝绥螨均有显著的吸引作用,排泄物对胡瓜钝绥螨没有明显的吸引作用。山楂叶螨对胡瓜钝绥螨的这种吸引作用说明胡瓜钝绥螨在田间具有自动搜索山楂叶螨的能力,可用于山楂叶螨的生物防治。     

东方钝绥螨对苹果园两种叶螨自然控制作用研究

本文报道了东方钝绥螨 Amblyseius orientalis 对苹果园里的苹果全爪螨 Panonychusulmi 和山楂叶螨 Tetranychus viennensis 的自然控制效果。在全年不喷杀螨杀虫剂的情况下,按照与苹果全爪螨成若螨1:57—73比例释放东方钝绥螨获全年叶片受害程度为5.5±3.1%,每叶有苹果全爪螨成螨0.3±0.2头、山楂叶螨0.1±0.2头和东方钝绥螨0.3±0.2头,相对防治效果为93.4%。还报导了东方钝绥螨和苹果全爪螨田间种群数量自然消长动态。     

伪钝绥螨的花粉饲养研究

在饲养伪钝绥螨的10种植物花粉中,核桃、洋槐、贴梗海棠、仙人球、迎春和卷心菜等可使其取食并完成发育,前4种还可使其产卵。花粉粒的外部形态结构,是影响该螨能否取食的重要因素之一。用桃、苹果花粉饲养伪钝绥螨,对其F_1代生活力有明显的不良影响。取食桃花粉的F_1代,幼螨间相互残杀,并取食未孵化的卵,使成活率降低。取食苹果花粉的F_1代可正常完成发育,但成螨性比降低,产卵时自食其卵。试验表明,单纯喂食桃或苹果花粉难以进行伪钝绥螨的累代饲养。     

虚伪钝绥螨的食性研究

在26℃恒温、相对湿度75％的条件下,虚伪钝绥螨取食朱砂叶螨、山楂叶螨和苹果叶螨的发育历期分别为6.08、6.68、6.54天,一生产卵总量分别为47.52、48.75、48.12粒。上述食料对虚伪钝绥螨的发育和生殖无明显差别。通过对22种植物花粉的筛选,证明其中18种花粉可使虚伪钝绥螨完成发育,12种可以使其产卵,而以苹果、桃的花粉最能满足虚伪钝绥螨的生育要求,如进一步明确这两种花粉对虚伪钝绥螨的后代生育能力无不良影响,就有可能用花粉来取代叶螨,在室内大量繁殖。     

伪钝绥螨对叶螨与花粉的选择性

用朱砂叶螨和苹果或桃花粉同时喂饲伪钝绥螨,其若螨和雌成螨对朱砂叶螨有较强的选择性。叶螨的丝和粪的引诱作用增强了这种选择性。幼螨活动性差,取食量极小,对2类食物的选择性不明显。     

湿度对植绥螨存活和繁殖影响研究

在恒温27±1℃下比较湿度对真桑钝绥螨、锯齿盲走螨、江原钝绥螨和尼氏真绥螨存活和繁殖的影响。初步认为:供试对象均属于喜湿种类,饲养时相对湿度应控制在85—95％之间,实行果园植被覆盖,可以提高地面湿度,有利于植绥螨的生存和繁衍。 低湿环境对真桑钝绥螨的存活和繁殖影响最大,这与系统调查结果相一致。     

应用伪钝绥螨防治苹果全爪螨初报

伪钝绥螨是叶螨的有效天敌,1986年引入天水,1987、1988年在苹果园进行了释放试验,6月中、下旬按1:50的益害比释放于苹果树上,结果表明经过35～40天,苹果全爪螨从峰期(1987、7、31)7.79头/叶和(19888、2)21.84头/叶开始减退,至50～60天,分别减退为1.76头/叶和1.90头/叶,而对照树分别为19.43头/叶(8、20)和44.15头/叶(8、2)。该螨对苹果全爪螨越冬卵的控制效果明显,释放树平均一年生枝条单枝含卵量为15.95粒,化防树为93.64粒。     

苹果全爪螨抗药性研究进展

苹果全爪螨是重要的蔷薇科果树害虫之一, 具有体型小、繁殖快、世代周期短等生物学特点。该螨以若螨和成螨取食苹果叶片和嫩芽, 影响果树生长发育, 造成果实品质和产量下降, 自20世纪60年代开始其在我国果园的发生逐年加重。国内外对苹果全爪螨的防治主要采用化学杀螨剂, 由于化学杀螨剂长期不规范的使用, 致使该螨对多种类型的化学杀螨剂产生了抗药性。本文总结了苹果全爪螨的发生、为害、抗药性现状及其抗性机理, 同时结合国内外苹果全爪螨抗药性和防治相关研究, 提出该螨抗药性治理策略, 以期为其防治提供参考。     

An index for selective toxicity of miticides to phytophagous mites and their predators based on orchard trials

Data from nine trials conducted from 1990 to 1998 in apple orchards in Nova Scotia and Quebec, Canada, were used to estimate the predator-prey selectivity of miticides and their potential compatibility with biological control of mites. The European red mite Panonychus ulmi (Koch) was the dominant and more harmful phytophagous species, followed by the apple rust mite, Aculus schlechtendali (Nalepa). Two predacious mites, the phytoseiid, Typhlodromus pyri Sheuten, and the stigmaeid, Zetzellia mali (Ewing), were often found in the orchards. We used one minus the ratio of mite-days in treated plots to those in the control plots as an index of population suppression and toxicity of the miticides. Miticides were then categorized into classes similar to those employed by the International Organization for Biological Control to rate pesticide toxicity to natural enemies of insect and mite pests. Selectivity of miticides was mostly based on toxicity to P ulmi, the major pest, versus toxicity to T pyri, the major predator, with some consideration of the two lesser species, A schlechtendali and Z mali. In most cases, our findings were in accord with other studies. Abamectin and clofentezine had favourable selectivity (more toxic to the two phytophagous mites than to T pyri). The higher recommended rate of pyridaben (450 g ha(-1)) and two rates of spirodiclofen (180 and 240 g ha(-1)) were neutral (equally toxic to pests and predators). The lower rate of pyridaben (216 g ha(-1)), dicofol, formetanate hydrochloride and propargite were unfavourably selective (more toxic to T pyri). A higher than recommended rate of pyridaben (2160 g ha(-1)) applied before bloom was disruptive--P ulmi-days after treatment were actually greater than with the untreated control. P ulmi resistance to dicofol and propargite were probable complicating factors in some of the orchard trials.     

Positive correlations between in vitro growth rate and pathogenesis in Ophiostoma ulmi

In vitro radial growth rate was found to be positively correlated with pathogenicity in two experiments involving NAN aggressive isolates of O. ulmi. One of the correlations was detected in a sample of B mating-type, but not in equivalent A mating-type isolates. Another was detected in a sample of pathogenic phase, but not in comparable saprophytic phase isolates. The significance of these results for the vascular wilt syndrome and population biology of O. ulmi is discussed.     

Assessment of Phaeotheca dimorphospora for biological control of the Dutch elm disease pathogens, Ophiostoma ulmi and O. novo-ulmi

The antagonistic fungus Phaeotheca dimorphospora was tested as a biocontrol agent against the Dutch elm disease pathogens, Ophiostoma ulmi and O. novo-ulmi , on Ulmus americana seedlings in the glasshouse. Curative inoculation of seedlings with P. dimorphospora had no significant effect on disease development. Conditioning inoculation of seedlings with the antagonist protected them against O. ulmi but not against the more aggressive O. novo-ulmi . In seedlings challenged with both the antagonist and either one of the pathogens, P. dimorphospora only spread locally around the inoculation point; however, the pathogens spread systemically throughout the whole plant. O. novo-ulmi survived in the region colonized by P. dimorphospora , whereas O. ulmi did not.     

5种杀螨剂对苹果树红蜘蛛田间药效评价

测定了5种不同作用机理杀螨剂对苹果树红蜘蛛的防效,并对其田间的应用效果进行评价。结果表明：三唑锡20%悬浮剂800倍液和唑螨酯5%悬浮剂2 000倍液速效性和持效性均较好,药后2d防效即达到90%以上,持效期25d以上;矿物油99%乳油和阿维菌素1.8%乳油速效性一般,但持效期较长,也能达到25d以上,哒螨灵40%可湿性粉剂防效一般。三唑锡20%悬浮剂和唑螨酯5%悬浮剂可快速有效地防治苹果树红蜘蛛,对天敌等影响较小,对作物安全,可作为防治的首选药剂使用。     

8种杀螨剂对苹果树红蜘蛛田间防效评价

对8种不同作用机理杀螨剂防治苹果树红蜘蛛的效果进行了田间试验。结果表明：三唑锡、炔螨特、哒螨灵、阿维菌素、唑螨酯、螺螨酯、双甲脒、甲氰菊酯均是防治苹果树红蜘蛛的有效药剂,以螺螨酯的总体防效最好。     

东方钝绥螨生物学及食量研究

东方钝绥螨在昌黎地区每年大约发生23代左右。平均卵期1.8天,幼虫期0.7天、第一若虫期1.0天、第二若虫期1.5天、产卵前期4.6天、产卵期15.2天、雌成螨期20.5天、雄成螨期15.6天、生命周期为25.5天。平均日产卵量1.5粒,总卵量16粒。雌成螨日捕食山楂叶螨卵3粒及若螨0.46头,总捕食量为卵91粒,若螨43头及成山楂叶螨14头,对苹果全爪螨捕食量3.4头(雌成螨)。     

1990年人工释放中华卵索线虫防治麦田一代粘虫试验

在1988年释放试验基础上,1990年5月,在土中中华卵索线虫成虫密度为14.7条/m~3,粘虫密度为45头/m~2的情况下,按益害比100:1释放线虫感染期幼虫于144m~2麦田中,防冶一代粘虫幼虫。24小时后即获寄生率高峰76％,校正寄生率为52％。麦收后挖方调查,释放区土中寄生线虫密度比释放前增加2.7倍。试验表明在田间寄生线虫成虫基数为10～20条/m~3的地区,按益害比100:1释放线虫,释放前后浇水,保持土壤湿度,创造良好寄生条件,既可在当年有效地减轻粘虫为害,又可较快地提高土中寄生线虫密度,提高今后常年自然控制粘虫的能力。     

Development of ELISA for Ophiostoma ulmi using antigen-coated wells

A simple sensitive ELISA test has been developed that detects either antibodies to, or antigens of, the Dutch elm pathogen, Opiostoma ( Ceratocystis ) ulmi. The dilution end point for antisera raised in mice againt mycelial antigens was 10^-4. The minimum amount of antigen detected in wells passively coated with antigens solubilized in phosphate-buffered saline was 500 pg/ml. The assay proved an effective method for rapidly screening large numbers of hybridoma supernatants for monoclonal antibodies that recognize O. ulmi antigens and it has also been used to detect fungal antigens in saline extracts of diseased tissue. Non-specific binding of preimmune antiserum to plant extracts was markedly higher in extracts from diseased than non-diseased tissue. Production by the diseased host or by the pathogen in vivo of a protein A or lectin-like molecule that binds non-specifically to immunoglobulins is postulated.