Ultrasonographic examination of the distal limb of cattle.

Ultrasonography has proven its worth in both small animal and equine musculoskeletal work. It allows visualization of soft tissue that is not possible with anything barring magnetic resonance imaging. In bovine practice, ultrasound has been used to great effect in reproductive work and has also shown itself to be of value in the evaluation of the thorax, abdomen, and the udder. Ultrasound examination of the bovine limb is certainly feasible because of the availability of small portable and affordable equipment. The restraint necessary is dependent on the animal. Cattle, because of their size and inherent dislike of restraint, pose a challenge for ultrasonographic examination. However, if proper restraint is used, it seems possible that ultrasonography may become a helpful tool in the evaluation of soft tissue injuries. It should prove to provide valuable information that may be used to offer better advice with regard to treatment and prognosis.     

Challenges of Cardiopulmonary Bypass—A Review of the Veterinary Literature

Cardiopulmonary bypass (CPB) has been used in veterinary medicine in experimental surgery and to address congenital and acquired diseases. We review the veterinary literature and expose common challenges of CPB in dogs and cats. Specifically, we describe the most specific elements of this technique in veterinary patients. The variety in animal size has made it difficult to standardize cannulation techniques, oxygenators, and priming volumes and solutions. The fact that one of the most common cardiovascular disorders, mitral valve disease, occurs predominantly in small dogs has limited the use of bypass in these patients because of the need for small, low prime oxygenators and pumps that have been unavailable until recently. Coagulation, hemostasis, and blood product availability have also represented important factors in the way CPB has developed over the years. The cost and the challenges in operating the bypass machine have represented substantial limitations in its broader use.     

Radiography,Computed Tomography and Magnetic Resonance Imaging of Craniofacial Structures in Pig

The pig has recently become popular as a large animal experimental model in many fields of biomedical research. The aim of this study is to evaluate the basic anatomical structures in the head region of the pig to lay the groundwork for its practical clinical usage or pre‐clinical research in the future. We used three different diagnostic imaging methods: radiography, computed tomography (CT) and magnetic resonance imaging (MRI). The analysis showed that radiographic imaging is suitable only for general evaluation of the facial area of the pig skull. CT images showed excellent spatial definition of bony structures of the whole craniofacial area, and MRI images revealed fine soft tissue details. Radiography is preferentially suited to general assessment of bone structures of the facial skeleton; however, the thick layer of adipose tissue in the craniofacial region of the pig makes the imaging of some parts difficult or even impossible. CT is useful for revealing morphological details of mineralized tissues, whereas MRI is more suitable for soft tissue analysis and the detection of subtle pathologic changes in both bone and soft tissues. Therefore, before using pigs as an experimental model in craniofacial research, it is necessary to evaluate the suitability and disadvantages of potential imaging methods and how appropriate they are for accurate visualization of desired structures.     

Principles and applications of the balanced steady-state free precession sequence in small animal low-field MRI

Magnetic resonance imaging (MRI) in small animal practice is largely based on classic two-dimensional spin-echo, inversion recovery and gradient-echo sequences which are largely limited by low spatial resolution, especially in low-field (LF)-MRI scanners. Nowadays, however, the availability of volumetric sequences can open new perspectives and enhance the diagnostic potential of this imaging modality. Balanced steady-state free precession (bSSFP) is a three-dimensional gradient-echo sequence in which image contrast is given by the ratio of T2 and T1, resulting in low soft-tissue signal, poor cerebral grey/white matter distinction and a bright signal from free fluid and fat. Such properties, along with a high signal-to-noise ratio and a very high spatial resolution deriving from acquisition of contiguous blocks of data, make this sequence perfectly suited for morphologic imaging, particularly for fluid-containing structures. Although bSSFP is widely adopted in human medical imaging, the use of this sequence in veterinary radiology is limited to anatomic studies of the inner ear and quadrigeminal cistern. This review aims to discuss the technical background of the bSSFP sequence and its possible advantageous applications in small animal LF-MRI for different specific disorders of the spine (arachnoid diverticula, small disc herniation, facet joint synovial cysts), brain (supracollicular fluid accumulation, traumatic injuries) and ligaments (complete and partial tears).     

Recent advances in soft tissue minimally invasive surgery

Advancements in soft tissue minimally invasive surgery have been rapid and comprehensive since its inception in human medicine approximately 25 years ago. From its origins in traditional laparoscopy, followed rapidly by video‐assisted thoracoscopic surgery, minimally invasive surgery in human medicine has evolved through a number of different platforms that now include single‐port approach devices, robotic surgery as well as natural orifice transluminal endosurgery. Whilst some of these remain beyond the reach of veterinary medicine for now, largely because of technical challenges and the prohibitive costs of some single‐use disposable components, veterinary minimally invasive surgery is advancing rapidly allowing our small animal patients to benefit from some of the many documented advantages that a minimally invasive approach affords the patient .     

A review of Doppler sonography for the assessment of tumour vascularity

During the past decade, the vascular biology of cancer has become a highly interesting research field. To assess tumour vascularity and perfusion, various strategies such as computed tomography, magnetic resonance imaging and positron emission tomography have been investigated. Over the last years, important advances have taken place in the Doppler technology which dramatically improved its ability to evaluate the vascular system, even small and deep vessels. Doppler sonography provides a non‐invasive means to assess the extent and morphology of tumour vascularity. This information is clinically important regarding diagnosis, prognosis and response to treatment, particularly in connection with the development of novel angiogenesis inhibitors. This article describes the different Doppler technologies most commonly used in human and experimental animal oncology. Based on in vivo tumour models and extensive clinical experience in humans, their significance and potential clinical applications are illustrated. First clinical reports in veterinary medicine are also reviewed.     

The spine

Computed tomography (CT) and magnetic resonance imaging (MRI) are becoming increasingly accessible in veterinary medicine. Because of their ability to image the bony spine and spinal cord noninvasively and with exceptional detail, these techniques have revolutionized the way spinal disorders are diagnosed in both people and animals. Although the veterinary literature on this topic is limited, interpretation of CT and MR images of the spine are facilitated by the similarity of human and animal disease processes. This article provides an overview of imaging strategies, normal anatomy, and the CT and MRI features of degenerative, infectious, neoplastic, and vascular diseases of the spine.     

What’s happened to Staphylococcus intermedius? Taxonomic revision and emergence of multi‐drug resistance

Staphylococcus intermedius has been the predominant coagulase-positive Staphylococcus isolated from canine skin and mucosae and the most commonly reported staphylococcal pathogen in small animal practice for the last 35 years. Although microbiological tests have historically indicated variability in biochemical characteristics amongst S. intermedius isolates from animals, an acceptable level of diagnostic accuracy for clinical purposes was readily achievable with routine phenotypic testing. However, three recent developments have changed our understanding of the term "S. intermedius" and have challenged veterinary bacteriologists to ensure correct species identification of pathogenic staphylococci from small animals. First, the increasing recognition of meticillin-resistant Staphylococcus aureus in small animal practice and its human health implications demand accurate species identification. Secondly, the application of molecular techniques to analysis of staphylococcal isolates has led to a revised taxonomy and canine isolates of S. intermedius being re-named S. pseudintermedius. Thirdly, the recent, rapid emergence of meticillin- and multi-drug-resistant strains of Staphylococcus pseudintermedius (MRSP) has become a major therapeutic challenge in veterinary practice worldwide, including the UK. This article discusses the background of the recent taxonomic changes within the genus Staphylococcus and reviews the key features of MRSP and its implications for day-to-day laboratory diagnosis and small animal practice.     

Applications of enzyme-linked immunosorbent assay in veterinary medicine: A bibliography

During the last decade enzyme-linked immunosorbent assay has been a technique of major interest to those engaged in immunodiagnostics of human and animal diseases. Owing to its simplicity, specificity and sensitivity it has taken precedence over other conventional assays, including radioimmunoassay on the grounds of freedom from radiation hazards. Many applications of this assay have been developed in veterinary medicine and they are listed in this article.     

超高效液相色谱技术在兽药分析领域应用的研究进展

超高效液相色谱技术是近年来迅速发展的基于小颗粒填料的液相色谱技术,具有快速、灵敏、准确、高效等众多优点,已成为药物检测和分析领域的研究热点之一。随着该技术的不断发展,其已在兽药研究开发和兽药残留检测等领域得到了较为广泛的应用。文章就超高液相色谱的技术特点及其实际应用进行综述,并对该项技术在未来兽药分析领域广阔的应用前景进行了展望。     

Contrast ultrasound: general principles and veterinary clinical applications

The concept of contrast enhancement has significantly extended the usefulness of ultrasound imaging in human medicine and medical research over the past decade. The persistence and efficacy of ultrasound contrast agents has been improved and specific imaging sequences have been developed. Contrast ultrasound provides Doppler and grey-scale enhancement. Doppler examinations are improved when studying deep or small vessels and vessels with low flow velocities. Specific contrast imaging sequences allow detection of tissue enhancement with grey-scale ultrasound which enables assessment of tissue perfusion. Major clinical applications of contrast ultrasound in the human medicine field are the heart, the parenchymal organs such as the liver, spleen and kidneys, and vascular applications. Many other interesting applications have been identified and beside their diagnostic value, intensive research is currently investigating the use of ultrasound contrast agents for therapeutic applications such as targeted delivery of drug- or gene-loaded microbubbles. In the last few years, contrast ultrasound has also been introduced in veterinary medicine. Its usefulness has been shown in diseases of the liver, spleen, kidney, pancreas, lymph nodes and superficial tumours. In the present article, an overview of the physical principles, imaging techniques and image analyses is presented. In addition, a literature review details the current use in veterinary medicine and areas of potential utilization are discussed.     

Thromboelastography: a tool for measuring hypercoagulability, hypocoagulability, and fibrinolysis

Objective: To describe the technique of thromboelastography (TEG) and review the applications of this coagulation test in humans and small animals. Data sources: Data sources included scientific reviews and original research publications. Human data synthesis: TEG in humans has been used for documentation of hypercoagulable and hypocoagulable states and has been shown to be beneficial in patient management. Veterinary data synthesis: Clinical evaluation of TEG in veterinary medicine is limited; however, recent reports have documented evidence of hypercoagulability in dogs with parvovirus and protein‐losing nephropathy. Additionally, many of the research models may be relevant to veterinary patients. Conclusions: TEG provides information about coagulation that is not available through routine coagulation tests. The application of TEG monitoring to veterinary patients shows promise; however, prospective clinical studies are needed.     

Apoptosis: A review of pro‐apoptotic and anti‐apoptotic pathways and dysregulation in disease

Objective – To review the human and veterinary literature on the biology of apoptosis in health and disease. Data Sources – Data were examined from the human and veterinary literature identified through Pubmed and references listed in appropriate articles pertaining to apoptosis. Human Data Synthesis – The role of apoptosis in health and disease is a rapidly growing area of research in human medicine. Apoptosis has been identified as a component of human autoimmune diseases, Alzheimer's disease, cancer, and sepsis. Veterinary Data Synthesis – Research data available from the veterinary literature pertaining to apoptosis and its role in diseases of small animal species is still in its infancy. The majority of veterinary studies focus on oncologic therapy. Most of the basic science and human clinical research studies use human blood and tissue samples and murine models. The results from these studies may be applicable to small animal species. Conclusions – Apoptosis is the complex physiologic process of programmed cell death. The pathophysiology of apoptosis and disease is only now being closely evaluated in human medicine. Knowledge of the physiologic mechanisms by which tissues regulate their size and composition is leading researchers to investigate the role of apoptosis in human diseases such as cancer, autoimmune disease and sepsis. Because it is a multifaceted process, apoptosis is difficult to target or manipulate therapeutically. Future studies may reveal methods to regulate or manipulate apoptosis and improve patient outcome.     

Animal welfare education: development and prospects

Animal welfare has developed rapidly as a scientific discipline since the 1980s. Concepts have been refined, methodologies for assessment developed, and links made to other areas of science. Changes in the subject and in its teaching are required. Since 1986, a series of senior academic teaching posts in the subject have been created, especially in the last 10 years. Veterinary and animal science students should receive a specific course on animal welfare, in addition to mention of the subject in other courses. In the future, more allusion to developments in understanding of welfare in relation to disease and brain measures of welfare is likely. The central role of animal welfare in veterinary and animal science teaching will become more firmly established.     

Use of magnetic resonance imaging for the investigation of orbital disease in small animals

Twenty-five small animal patients presenting with signs of orbital disease were investigated using magnetic resonance imaging (MRI) in an attempt to assess the value of this imaging technique for diagnosis. All patients were also examined using ultrasonography, and skull radiography was performed in 20 of these animals. The final diagnoses included neoplasia, inflammatory disease and foreign body penetration. MRI produced detailed images of orbital tissues and provided more information about the extent of pathology than the other imaging techniques; a correct diagnosis based solely on the MRI scan was made in 22 cases. Radiography was found to be helpful only in cases in which neoplastic disease extended markedly beyond the confines of the orbit into the nasal chamber and paranasal sinuses. Radiographic changes other than soft tissue swelling were not evident in other orbital disease processes. Ultrasonography gave both false negative and false positive diagnoses for neoplastic masses, although it allowed the correct diagnosis of both cases of foreign bodies and one of the three cases of retrobulbar abscesses in this series. MRI is recommended for patients in which radiography and ultrasonography fall to produce a confident diagnosis or for which surgery is proposed.     

Comparison of high‐field MRI and multidetector CT for grading Chiari‐like malformation and syringomyelia in Cavalier King Charles Spaniels

Chiari‐like malformation (CM) and syringomyelia (SM) are common illnesses that can cause debilitating neuropathic pain in Cavalier King Charles spaniels (CKCS). The current imaging modality to screen CKCS for CM/SM is MRI of the brain and cervical spine. Magnetic resonance imaging provides good soft tissue detail and contrast of the cerebellum and cervical spinal cord. Computed tomography (CT) is another cross‐sectional imaging technique that facilitates brain and neck evaluation; however, soft tissue resolution does not match that of MRI. Computed tomography benefits include identification of concurrent craniocervical junction anomalies (atlantooccipital overlap) and shorter imaging/anesthesia times with the ability to use only sedation. The aim of this retrospective, method comparison study is to assess the utility of multidetector CT for screening CM and SM in CKCS as compared to high‐field MRI. Three groups of observers with different levels of experience graded CM and SM based on the British Veterinary Association/Kennel Club CM/SM classification criteria. Thirty CKCS underwent multidetector CT and 3 Tesla MRI studies. Computed tomography and MRI studies were reviewed at different timepoints to minimize bias. Computed tomography has lower Cohen's Kappa agreement for each observer group compared to MRI. The intraclass correlation coefficient averaging CM and SM for all groups was excellent using MRI, while CT was poor for SM and moderate for cerebellar herniation. Greater observer experience resulted in a higher agreement for CT and MRI. Magnetic resonance imaging should remain the standard for screening of CM and SM as CT can result in misclassification and greater disagreement.     

If you build it,they will learn: A review of models in veterinary surgical education

Surgical skills are learned through deliberate practice, and veterinary educators are increasingly turning to models for teaching and assessing surgical skills. This review article sought to compile and review the literature specific to veterinary surgical skills models, and to discuss the themes of fidelity, educational outcomes, and validity evidence. Several literature searches using broad terms such as “veterinary surgery model,” “veterinary surgical model,” and “veterinary surgical simulator” were performed using PubMed, CAB abstracts, and Google scholar. All articles describing a model created and utilized for veterinary surgical training were included. Other review articles were used as a source for additional models. Commercially available models were found using review articles, internet browser searches, and communication with veterinary clinical skills educators. There has been an explosion of growth in the variety of small animal surgical task trainers published in the last several decades. These models teach orthopedic surgery, ligation and suturing, open celiotomy and abdominal surgery, sterilization surgeries, and minimally invasive surgeries. Some models were published with accompanying rubrics for learner assessment; these rubrics have been noted where present. Research in veterinary surgical models is expanding and becoming an area of focus for academic institutions. However, there is room for growth in the collection of validity evidence and in development of models for teaching large animal surgery, training surgical residents, and providing continuing education to practitioners. This review can assist with evaluation of current surgical models and trends, and provide a platform for additional studies and development of best practices.     

Chelonian reproductive disorders.

The study of reproductive diseases of chelonians has become increasingly sophisticated in the last decade. Widespread captive breeding has increased the number of reproductive problems presented to veterinarians. Advances in the level of veterinary care have encouraged chelonians owners to seek solutions to problems that may have previously been considered unsolvable. Improved diagnostic imaging, particularly radiography, ultrasonography and endoscopy, have made visualization and diagnosis of reproductive disease easier. Better quality veterinary care has made treatment of chelonian diseases more sophisticated which has lead to improved methods of anesthesia, surgery, and medicine. Concerns over rapidly diminishing chelonian populations and habitat have made the need for study of reproduction a higher priority; veterinary medicine has gained from this research.     

Magnetic resonance imaging features of an extranodal T cell rich B cell lymphoma in the pharyngeal mucosa in a horse

An 11‐year‐old Warmblood gelding was presented for inspiratory stridor and dysphagia. Based on history and clinical examination, a solitary mass localised in the oropharynx was suspected. Due to its inaccessibility and defensive behaviour of the horse, it was difficult to visualise this mass either by upper airway endoscopy or by oral examination and the conventional imaging methods (radiology and ultrasound) provided only limited information. Fine needle aspiration cytology was suggestive of lymphoma, but the exact localisation and the extent of tissue infiltration of the tumour could only be defined by magnetic resonance imaging (MRI). MRI has proved to be a very useful diagnostic tool in equine lameness investigation and, as this case illustrates, it has considerable diagnostic potential for soft tissue examination of the equine head.