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猪干扰素-γ基因单核苷酸多态性分析
引用本文:王大力,刘畅,王秀娜,黄大鹏,孙博兴,赵志辉.猪干扰素-γ基因单核苷酸多态性分析[J].中国畜牧兽医,2009,36(6):90-94.
作者姓名:王大力  刘畅  王秀娜  黄大鹏  孙博兴  赵志辉
作者单位:(1.吉林大学畜牧兽医学院, 长春 130062; 2.黑龙江八一农垦大学 动物科技学院, 大庆 163319)
基金项目:吉林省科技发展计划项目 
摘    要:干扰素-γ(interferon-gamma,IFN-γ)在调节机体免疫应答及抵抗病毒感染等方面有着重要的作用。为探索IFN-γ基因作为猪抗病育种中一种分子标记的可能性,采用PCR-SSCP及测序方法对包括军牧1号白猪、杜洛克猪、约克夏猪3个品种共481个个体的IFN-γ基因全序列的单核苷酸多态性进行分析。结果在猪IFN-γ基因的内含子1、内含子3、外显子4上各发现了一个突变位点,分别为T825C、T2730C、G5301A。除在约克夏猪中没有检测到G5301A突变外,各遗传群体内T825C、T2730C、G5301A位点均处于Hardy-Weinberg平衡状态。各个突变位点的多态信息含量均小于0.25,呈低度多态,说明猪IFN-γ基因较保守。

关 键 词:  干扰素-γ  单核苷酸多态性  

Single Nucleotide Polymorphism Analysis of IFN-γ Gene in Pigs
WANG Da-li,LIU Chang,WANG Xiu-na,HUANG Da-peng,SUN Bo-xing,ZHAO Zhi-hui.Single Nucleotide Polymorphism Analysis of IFN-γ Gene in Pigs[J].China Animal Husbandry & Veterinary Medicine,2009,36(6):90-94.
Authors:WANG Da-li  LIU Chang  WANG Xiu-na  HUANG Da-peng  SUN Bo-xing  ZHAO Zhi-hui
Affiliation:(1.College of Animal Science and Veterinary Medicine of Jilin University, Changchun 130062,China; 2.College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, China)
Abstract:Interferon-gamma (IFN-γ) plays an important role in the innate host defense against viral infection. In order to analyze the value that the IFN-γ gene act as a genetic marker in porcine disease resistance, single nucleotide polymorphisms (SNPs) in the whole region of the IFN-γ gene were studied by use of PCR-SSCP approach in 481 pigs from the “Junmu No. 1” White, Landrace, Duroc pigs. Three SNP sites were detected: T825C in Intron 1, T2730C in Intron 3 and G5301A in Extron 4. Except for the absence of G5301A in Duroc pigs, all the other sites in the all populations were in a state of Hardy-Weinberg equlibrium.The PIC of every sites had low polymorphisms(<0.25), which represented the own unique conservativeness of IFN-γ gene in pigs.
Keywords:pig  IFN-γ  single nucleotide polymorphisms (SNP)
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