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猪乙型脑炎病毒E基因的克隆与序列分析
引用本文:丛晓燕,吴家强,王怀忠,徐文,张秀美,王金宝,陈溥言.猪乙型脑炎病毒E基因的克隆与序列分析[J].中国畜牧兽医,2009,36(9):71-73.
作者姓名:丛晓燕  吴家强  王怀忠  徐文  张秀美  王金宝  陈溥言
作者单位:(1.山东省农业科学院畜牧兽医研究所, 济南 250100; 2.青岛农业大学, 青岛 266109; 3.南京农业大学动物医学院, 南京 210095)
摘    要:根据GenBank中猪乙型脑炎病毒SA14-14-2基因序列设计2对引物,从分离的猪源乙型脑炎病毒SD-001株的细胞培养物中扩增出包括E基因全长的两段基因,将扩增的目的片段进行克隆与序列分析。结果表明,所克隆的E基因编码结构域(Domain)区段与SA14-14-2、P3、Beijing-1等毒株的核苷酸与氨基酸序列同源性分别达97.2%与96.8%以上,属于Ⅲ型乙型脑炎病毒,与疫苗株SA14-14-2的Domain区相比,共有8个氨基酸位点变异。

关 键 词:猪乙型脑炎病毒  E基因  序列分析  

Cloning and Sequence Analysis of E Gene of Japanese Encephalitis Virus
CONG Xiao-yan,WU Jia-qiang,WANG Huai-zhong,XU Wen,ZHANG Xiu-mei,WANG Jin-bao,CHEN Pu-yan.Cloning and Sequence Analysis of E Gene of Japanese Encephalitis Virus[J].China Animal Husbandry & Veterinary Medicine,2009,36(9):71-73.
Authors:CONG Xiao-yan  WU Jia-qiang  WANG Huai-zhong  XU Wen  ZHANG Xiu-mei  WANG Jin-bao  CHEN Pu-yan
Affiliation:(1.Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2.Qingdao Agricultural University, Qingdao 266109, China; 3.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China)
Abstract:According to the published E genes sequence of JEV in GenBank, two pairs of PCR primers were designed. E genes were amplified from JEV-SD001 strain. Then the PCR products were cloned into pMD-18 Easy Vector, and the recombinant plasmids were obtained. The sequence of the cloned PCR products were analyzed with other JEV isolates in GenBank. The results indicated that the homology of their nucleotide sequence was over 97.2%, and it shared over 96.8% of amino acid homology with other JEV isolates. JEV-SD001 belonged to Japanese encephalitis virus genotype Ⅲ, E protein of isolates showed some differences as compared with vaccine strains.
Keywords:Japanese encephalitis virus  E gene  sequence analysis
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