| 鱼源小肠结肠炎耶尔森氏菌的耐药性研究 |
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| 引用本文： | 王利,苟小兰,龙钟明,刘港彪.鱼源小肠结肠炎耶尔森氏菌的耐药性研究[J].中国畜牧兽医,2012,39(12):36-40. |
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| 作者姓名： | 王利 苟小兰 龙钟明 刘港彪 |
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| 作者单位： | 西南民族大学生命科学与技术学院,动物遗传育种学国家民委—教育部重点实验室,四川成都610041 |
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| 基金项目： | 四川省科技厅应用基础项目，西南民族大学研究生学位点建设项目 |
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| 摘 要： | 本试验采用聚合酶链反应(PCR)方法检测鱼源小肠结肠炎耶尔森氏菌的四环素类耐药基因(tetA、tetC、tetM)、磺胺类耐药基因(sul1、sul2、sul3)和氨基糖苷类耐药基因(aph(3')-Ⅲa、aac(6')-Ⅰb、ant(3')-Ⅰa、aac(3)-Ⅱa),并采用κ-B法检测该菌对14种抗生素的耐药表型。结果表明,小肠结肠炎耶尔森氏菌8种耐药基因(tetC、tetM、sul1、sul2,aph(3')-Ⅱa、aac(6')-Ⅰb、ant(3')-Ⅰa、aac(3)-Ⅱa)被检测出,而tetA、sul3基因未被检测出。该菌株对复方新诺明、磺胺异恶唑、红霉素、利福平、洁霉素和头孢噻吩6种抗生素耐药,对氟哌酸、氟苯尼考和头孢曲松等8种抗生素敏感。这为治疗小肠结肠炎耶尔森氏菌引起的鱼病积累了资料。
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| 关 键 词： | 小肠结肠炎耶尔森氏菌 耐药基因 耐药表型 |
| 收稿时间： | 2012-05-02 |
Drug Resistance Research on Yersinia enterocolitica Isolated from Fish |
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| WANG Li
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GOU Xiao-lan
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LONG Zhong-ming
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LIU Gang-biao.Drug Resistance Research on Yersinia enterocolitica Isolated from Fish[J].China Animal Husbandry & Veterinary Medicine,2012,39(12):36-40. |
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| Authors: | WANG Li GOU Xiao-lan LONG Zhong-ming LIU Gang-biao |
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| Affiliation: | Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission & Ministry of Education, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China |
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| Abstract: | It studied the drug resistance of Yersinia enterocolitica isolated from fish. The tetA,tetC,tetM,sul1,sul2,sul3,aph(3')-Ⅱa,aac(6')-Ⅰb,ant(3')-Ⅰa and aac(3)-Ⅱa genes were detected using PCR approach. The sensitivities to 14 kinds of antibiotics were evaluated by Kirby-Bauer disc diffusion method. The results showed that the tetA and sul3 genes were not checked out, while the other resistant genes could been seen. The strain was resistant to compound sulfamethoxazole, sulfafurazole, erythromycin, rifampicin, lincomycin and cefaloridine, while it was sensitive to norfloxacin, florfenicol, rocephin and so on. It will benefit the further study of drug-resistant mechanism of Yersinia enterocolitica. |
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| Keywords: | Yersinia enterocolitica drug resistance gene drug resistance phenotype |
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