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2010年上半年11省市规模猪场疫病监测与剖析
引用本文:吕超超,焦连国,张金双,张永灯,赵锐,闫国晖,赵亚荣.2010年上半年11省市规模猪场疫病监测与剖析[J].中国畜牧兽医,2011,38(2):165-167.
作者姓名:吕超超  焦连国  张金双  张永灯  赵锐  闫国晖  赵亚荣
作者单位:(大北农动物医学研究中心,北京 100097)
摘    要:为了解近期发病猪场几种病毒性疫病的流行状况,采用RT-PCR和PCR方法,对2010年1月至6月期间,来自11省(市) 50个猪场共147份病料进行猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)、伪狂犬病毒(PRV)和猪圆环病毒2型(PCV2)检测,并采用ELISA方法对其中26个猪场458份血清样品进行伪狂犬gE野毒抗体检测。结果表明,PCV2阳性率最高,为75.3%(70/93);PRRSV阳性率次之,为57.4%(78/136),HP-PRRSV阳性率为43.5%(54/124);CSFV和PRV的阳性率分别为37.7%(52/138)和21.0%(21/100);伪狂犬gE野毒抗体阳性率为16.4%(75/458)。同时检出2种及2种以上病毒的样品占46.9%(69/147),其中以HP-PRRSV+PCV2、CSFV+PCV2及CSFV+HP-PRRSV+PCV2三种形式最为常见。PRRSV(50.0%)是猪场繁殖障碍性疾病中的最常见病原;CSFV(56.0%)和PCV2(48.0%)是断奶前后仔猪腹泻的常见病原;HP-PRRSV(53.5%)、CSFV(50.7%)和PCV2(48.0%)是保育猪发生高热症的常见病原。该检测结果为猪场疫病的诊断与防控提供了参考。

关 键 词:猪瘟  猪繁殖与呼吸综合征  伪狂犬病  猪圆环病毒病  PCR  ELISA  检测猪瘟  猪繁殖与呼吸综合征  猪圆环病毒病  PCR  ELISA  检测  

Laboratory Detection and Analysis of Common Viral Pathogens of Pig Farms in 11 Provinces during the First Half of 2010 Year
LV Chao-chao,JIAO Lian-guo,ZHANG Jin-shuang,ZHANG Yong-deng,ZHAO Rui,YAN Guo-hui,ZHAO Ya-rong.Laboratory Detection and Analysis of Common Viral Pathogens of Pig Farms in 11 Provinces during the First Half of 2010 Year[J].China Animal Husbandry & Veterinary Medicine,2011,38(2):165-167.
Authors:LV Chao-chao  JIAO Lian-guo  ZHANG Jin-shuang  ZHANG Yong-deng  ZHAO Rui  YAN Guo-hui  ZHAO Ya-rong
Affiliation:(Dabeinong Research Center for Veterinary Medicine, Beijing 100097, China)
Abstract:To understand the recent prevalence of several viral diseases in sick pig farms,147 tissue specimens were collected from 50 farms in 11 provinces(cities) and examined during January 2010 to May 2010 by RT-PCR and PCR methods.And 458 serum samples from 26 farms were examined for pseudorabies gE antibodies by ELISA method.The positive rate of porcine circovirus type 2(PCV2) was highest with 75.3%(70/93).The positive rate of porcine reproductive and respiratory syndrome virus(PRRSV) followed with 57.4%(78/136)...
Keywords:CSF  PRRS  PR  PCV  PCR  ELISA  detection  
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