| 氟喹诺酮类药物的多残留酶免疫分析研究 |
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| 引用本文： | 潘孝成,祁克宗,朱良强,赵瑞宏.氟喹诺酮类药物的多残留酶免疫分析研究[J].中国畜牧兽医,2007,34(11):126-129. |
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| 作者姓名： | 潘孝成 祁克宗 朱良强 赵瑞宏 |
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| 作者单位： | 1.安徽省农科院畜牧兽医研究所，合肥 230031；2.安徽农业大学，合肥 230036；3.安徽省兽医站,合肥 230022 |
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| 摘 要： | 以制备的多抗血清为基础，建立了氧氟沙星（OFL）、二氟沙星（DIF）和诺氟沙星（NOR）的多残留间接竞争酶免疫吸附测定法(Ci ELISA法)。OFL、 DIF和NOR的标准曲线的线性回归方程分别为y=-0.1984x+0.9687 (r=0.9885)、y=-0.1738x+0.9150 (r=0.9985)、y=-0.2004x+0.9572(r=0.9954)；中值（IC50）分别为231.57、245.32 、186.14 ng/ml ；最低检测限（LOD）分别为1.62、1.86、1.07 ng/ml；标准曲线在2~10000 ng/ml之间均有良好的线性关系(r≥0.9885)。方法的批内CV≤5.44%,批间CV≤12.45%。当OFL、DIF或NOR以浓度15~10000 ng/ml在牛奶中添加时，药物能较好地被回收，回收率均在71.70%~94.53%之间。
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| 关 键 词： | 氟喹诺酮类药物 多残留 间接竞争ELISA法 |
| 文章编号： | 1671-7236（2007）11-0126-03 |
| 修稿时间： | 2007-04-23 |
Study on the Multi Residues of Fluoroquinolones by Enzyme Immunoassay |
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| PAN Xiao-cheng,QI Ke-zong,ZHU Liang-qiang,ZHAO Rui-hong.Study on the Multi Residues of Fluoroquinolones by Enzyme Immunoassay[J].China Animal Husbandry & Veterinary Medicine,2007,34(11):126-129. |
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| Authors: | PAN Xiao-cheng QI Ke-zong ZHU Liang-qiang ZHAO Rui-hong |
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| Affiliation: | 1.Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China;2.Anhui Agricultural University, Hefei 230036, China; 3. Anhui Veterinary Station, Hefei 230022, China |
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| Abstract: | The antiserum was prepared with the mixture of OFL-BSA,DIF-BSA and NOR-BSA.The Ci-ELISA method for the detection of ofloxacin,difloxacin and norfloxacin was established with it.To ofloxacin,difloxacin and norfloxacin.The regression equations about their standard curves were y=-0.1984x 0.9687(r=0.9885),y=-0.1738x 0.9150(r=0.9985) and y=-0.2004x 0.9572(r=0.9954),respectively.The 50% inhibition concentration(IC50) were 231.57,245.32 and 186.14ng/ml,respectively.The limits of detect(LOD) were 1.62,1.86 and 1.07ng/ml,respectively.The coefficients of variation for the Ci-ELISA method were less than 5.44% in intra-assay and 12.45% in inter-assay.The recoveries in milk ranged from 71.70% to 94.53%. |
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| Keywords: | fluoroquinolones multi residue Ci-ELISA |
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