| 瘤胃微生物定量方法的研究进展 |
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| 引用本文： | 郭同军,王加启,王建平,霍小凯,卜登攀,魏宏阳,周凌云,刘开朗.瘤胃微生物定量方法的研究进展[J].中国畜牧兽医,2009,36(4):19-24. |
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| 作者姓名： | 郭同军 王加启 王建平 霍小凯 卜登攀 魏宏阳 周凌云 刘开朗 |
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| 作者单位： | （1.中国农业科学研究院北京畜牧研究所 动物营养学国家重点实验室，北京 100193；2 新疆农业大学，乌鲁木齐 830052） |
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| 基金项目： | 国家奶业科技支撑计划 |
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| 摘 要： | 当前人们对瘤胃中微生物的认识只有10%～20%，不断改进研究技术和手段，才能大大推动瘤胃微生态领域的研究。作者阐述了瘤胃微生物传统定量方法（滚管计数法和最大或然数法）和分子生物学定量方法，如探针杂交技术、荧光原位杂交、DGGE、定量PCR和流式细胞计量术（flow cytometry )等的应用状况及其各自的优缺点。
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| 关 键 词： | 瘤胃微生物 滚管计数法 最大或然数法 Real-Time PCR 流式细胞计量术 |
Research Progress on Quantitative Method for Rumen Microorganism |
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| GUO Tong-jun,WANG Jia-qi,WANG Jian-ping,HUO Xiao-kai,BU Deng-pan,WEI Hong-yang,ZHOU Ling-yun,LIU Kai-lang.Research Progress on Quantitative Method for Rumen Microorganism[J].China Animal Husbandry & Veterinary Medicine,2009,36(4):19-24. |
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| Authors: | GUO Tong-jun WANG Jia-qi WANG Jian-ping HUO Xiao-kai BU Deng-pan WEI Hong-yang ZHOU Ling-yun LIU Kai-lang |
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| Affiliation: | (1.State Key Laboratory of Animal Nutrition ，Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193，China;2. Xinjing Agricultural University, Urumqi 830052,China) |
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| Abstract: | There are only 10%～20% microbes being learned form rumen by now. It is the necessary for promoting extensive and intensive research on rumen ecosystem to develop novel research method. This review discuss about the researched progress and their advantage or disadvantage of the quantitative method of traditional (roll-tube technique and most probable number) and modern molecular biology (nucleic acid hybridization, FISH, DGGE, quantification PCR and Flow cytometry). |
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| Keywords: | Real-Time PCR |
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