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| 不同绵羊群体间心脏型脂肪酸结合蛋白基因第2外显子单核苷酸多态性的研究 | |
| 引用本文： | 赵雪,曹阳,金海国,张立春,于永生,罗晓彤,李德允.不同绵羊群体间心脏型脂肪酸结合蛋白基因第2外显子单核苷酸多态性的研究[J].中国畜牧兽医,2013,40(4):32-35. |
| 作者姓名： | 赵雪 曹阳 金海国 张立春 于永生 罗晓彤 李德允 |
| 作者单位： | 1. 延边大学农学院,吉林延吉133002;吉林省农业科学院畜牧分院,吉林公主岭136100 2. 吉林省农业科学院畜牧分院,吉林公主岭,136100 3. 延边大学农学院,吉林延吉,133002 |
| 摘 要： | 以东北细毛羊、小尾寒羊、杜泊和道赛特4个群体为研究对象,采用PCR-SSCP方法对心脏型脂肪酸结合蛋白(heart fatty acid binding protein,H-FABP)基因第2外显子单核苷酸多态性进行检测。结果发现,H-FABP基因第2外显子存在939(A/G)、980(G/A)、1013(A/C)和1018(T/C)4个SNP位点,表现出AA、AB、BB 3种基因型;在东北细毛羊、小尾寒羊和杜泊群体中,BB型为优势基因型,在道赛特群体中,AB型为优势基因型;经χ2检验后,4个绵羊品种群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态;群体遗传多态性分析结果表明,东北细毛羊、杜泊和道赛特3个群体中的多态信息含量(PIC)均处于0.25和0.50之间,为中度多态,小尾寒羊为低度多态(PIC＜0.25);进一步分析结果表明,各个群体存在不同程度的遗传变异。 |
| 关 键 词： | 绵羊 心脏型脂肪酸结合蛋白 单核苷酸多态性 |
| 收稿时间： | 2012-12-13 |
Studies on Single Nucleotide Polymorphisms at the Exon 2 of H-FABP Gene among Different Sheep Breeds |
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| ZHAO Xue,CAO Yang,JIN Hai-guo,ZHANG Li-chun,YU Yong-sheng,LUO Xiao-tong,LI De-yun.Studies on Single Nucleotide Polymorphisms at the Exon 2 of H-FABP Gene among Different Sheep Breeds[J].China Animal Husbandry & Veterinary Medicine,2013,40(4):32-35. | |
| Authors: | ZHAO Xue CAO Yang JIN Hai-guo ZHANG Li-chun YU Yong-sheng LUO Xiao-tong LI De-yun |
| Affiliation: | 1. College of Agriculture, Yanbian University, Yanji 133002, China;2. Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China |
| Abstract: | PCR-SSCP approach was applied to test the single nucleotide polymorphisms (SNPs) at the exon 2 of heart fatty acid-binding protein (H-FABP) gene and four sheep populations that comprised of Northeast fine wool sheep, Small-Tailed Han sheep, Dorper sheep and Dorsets sheep were screened in this study. The results showed that four SNPs at 939(A/G), 980(G/A), 1013(A/C), 1018(T/C) and three genotypes were detected. During the Northeast fine wool sheep, Small-Tailed Han sheep and Dorper populations, the BB was the predominant genotype. While in Dorsets sheep, the AB was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP gene were in Hardy-Weinberg equilibrium. Statistical analysis revealed an intermediate polymorphism information content (PIC) in the Northeast fine wool sheep, Dorper sheep and Dorsets populations (0.25＜PIC＜0.50), but a low PIC in the Small-Tailed Han sheep population (PIC＜0.25). Further analysis showed that the different groups had different degree of genetic variation. |
| Keywords: | sheep heart fatty acid binding protein (H-FABP) SNPs |
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