| 胶体金标记抗圆弧青霉菌毒素—青霉酸单克隆抗体的研究 |
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| 引用本文： | 朱丽,雷红宇,袁慧.胶体金标记抗圆弧青霉菌毒素—青霉酸单克隆抗体的研究[J].中国畜牧兽医,2010,37(8):30-33. |
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| 作者姓名： | 朱丽 雷红宇 袁慧 |
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| 作者单位： | （湖南农业大学动物医学院， 长沙 410128） |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 本研究探讨了胶体金标记抗圆弧青霉菌毒素—青霉酸单克隆抗体探针的制备方法,摸索其最佳反应条件并对其活性进行检测。结果表明,胶体金标记抗青霉酸单克隆抗体的最佳pH为8.2,1mL胶体金标记的最小蛋白量为25μg。金标抗体与二抗发生牢固结合,即金标探针具有活性。该研究为青霉酸的胶体金免疫层析检测技术奠定了一定的基础。
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| 关 键 词： | 胶体金 抗青霉酸单克隆抗体 金标探针 |
Study on Colloidal Gold Labeled Monoclonal Antibody against Penicillic Acid from Penicillium cyclopium |
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| ZHU Li,LEI Hong-yu,YUAN Hui.Study on Colloidal Gold Labeled Monoclonal Antibody against Penicillic Acid from Penicillium cyclopium[J].China Animal Husbandry & Veterinary Medicine,2010,37(8):30-33. |
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| Authors: | ZHU Li LEI Hong-yu YUAN Hui |
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| Affiliation: | (College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China) |
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| Abstract: | Preparative method of colloidal gold labeled monoclonal antibody against penicillic acid (PA) from Penicillium cyclopium was established and its reaction condition was optimized. The activity of the gold-labeled probe was determinated. Results showed that the optimization pH was 8.2, the minimum proteins labeled 1 mL colloidal gold was 25 μg. It showed that the probe and anti-mouse anti-goat immnoglobulins were compaginated. The study provided foundation for colloidal gold immunochromotography assay against PA. |
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| Keywords: | colloidal gold monoclonal antibody against penicillic acid gold-labeled probe |
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