| 草地贪夜蛾病原白僵菌的分离鉴定及其致病力研究 |
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| 引用本文： | 邹春华,刘继辉,吴 伟,徐勋志,谢秀菊.草地贪夜蛾病原白僵菌的分离鉴定及其致病力研究[J].植物保护,2023,49(6):242-246. |
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| 作者姓名： | 邹春华 刘继辉 吴 伟 徐勋志 谢秀菊 |
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| 作者单位： | 1. 广州市农业科学研究院, 广州 510315; 2. 广州乾农农业科技发展有限公司, 广州 510315; 3. 云南守农生物科技有限责任公司, 昆明 650100 |
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| 基金项目： | 广州市基础研究计划(202102080121) |
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| 摘 要： | 草地贪夜蛾Spodoptera frugiperda (J. E. Smith)是一种全球性重大玉米害虫, 于2019年入侵我国云南并快速蔓延, 对我国的粮食安全造成重大威胁。本研究从玉米地块自然罹病的草地贪夜蛾幼虫僵虫中分离纯化致病菌, 并回接验证, 获得一株虫生真菌菌株, 结合形态学和rDNA-ITS序列分析, 确认该菌株属于球孢白僵菌Beauveria bassiana, 编号为SfG01。采用浸虫法测定了该菌株对草地贪夜蛾2龄幼虫的致病活性。结果表明, 孢子悬浮液浓度为1.00×109个/mL时, 草地贪夜蛾2龄幼虫第11天累计死亡率达86.67%, LC50为4.85×107个/mL, LT50为6.50 d。表现出一定的生防潜力, 具有进一步研究价值。
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| 关 键 词： | 草地贪夜蛾 球孢白僵菌 分离鉴定 致病力 |
| 收稿时间： | 2022/10/11 0:00:00 |
| 修稿时间： | 2022/12/8 0:00:00 |
Isolation and identification of entomopathogenic fungi Beauveria to Spodoptera frugiperda (Lepidoptera: Noctuidae) and its virulence |
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| ZOU Chunhu,LIU Jihui,WU Wei,XU Xunzhi,XIE Xiuju.Isolation and identification of entomopathogenic fungi Beauveria to Spodoptera frugiperda (Lepidoptera: Noctuidae) and its virulence[J].Plant Protection,2023,49(6):242-246. |
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| Authors: | ZOU Chunhu LIU Jihui WU Wei XU Xunzhi XIE Xiuju |
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| Affiliation: | 1. Guangzhou Academy of Agricultural Sciences, Guangzhou 510315, China; 2. Guangzhou Qiannong Agricultural Technology Development Co., Ltd., Guangzhou 510315, China; 3. Yunnan Shounong Bio-technology, Ltd., Kunming 650100, China |
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| Abstract: | Spodoptera frugiperda (J. E. Smith), a global major pest, invading into Yunnan province in 2019 and spreading quickly, has posed a great threat on food security of China. In this study, an entomopathogenic fungus strain named SfG01 was isolated from an infected S. frugiperda larvae in corn field, and then identified to be Beauveria bassiana based on morphological and rDNA-ITS sequence analysis. Virulence of the strain was tested by bioassay with impregnating inoculation to 2nd instar larvae of S. frugiperda. The result showed that the cumulative mortality was 86.67% at the 11th day after conidia inoculation at 1.00×109 spores/mL, the LC50 value was 4.85×107 spores/mL, and LT50 was 6.50 d. The strain SfG01 exhibits the bio-control potential against S. frugiperda and has the value for further research. |
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| Keywords: | Spodoptera frugiperda Beauveria bassiana fungal identification virulence |
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