| 亚洲玉米螟核型多角体病毒分离株鉴定及其对草地贪夜蛾的室内毒力测定 |
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| 引用本文： | 类承凤,姜干明,彭玲,杨健,胡葭,孙修炼.亚洲玉米螟核型多角体病毒分离株鉴定及其对草地贪夜蛾的室内毒力测定[J].中国生物防治学报,2019,35(5):741-746. |
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| 作者姓名： | 类承凤 姜干明 彭玲 杨健 胡葭 孙修炼 |
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| 作者单位： | 1. 中国科学院武汉病毒研究所, 武汉 430071;2. 湖北省黄冈市植保植检站, 黄冈 438000;3. 武汉市农业科学技术研究院蔬菜科学研究所, 武汉 430345 |
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| 基金项目： | 国家重点研发计划（2017YFD0201206） |
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| 摘 要： | 本文对从湖北省恩施州亚洲玉米螟幼虫上新分离的一种广谱病毒进行了形态学和分子特征鉴定，并以该病毒与另一种广谱杆状病毒对草地贪夜蛾幼虫进行了生物活性测定。结果表明新分离的核型多角体病毒与薄荷灰夜蛾核型多角体病毒和芹菜夜蛾核型多角体病毒具有较高的同源性，但遗传距离分析不支持它与这两种病毒为同一个种。生物活性测定表明，分离的病毒对草地贪夜蛾3龄幼虫的半数致死剂量是甘蓝夜蛾核型多角体病毒的3.86倍。本研究的结果为开发防治草地贪夜蛾的高效病毒杀虫剂提供了依据。
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| 关 键 词： | 核型多角体病毒 草地贪夜蛾 生物活性测定 |
| 收稿时间： | 2019-08-25 |
Identification of an Isolate of Ostrinia furnacalis Nucleopolyhedrovirus and Its Bioactivity against Spodoptera frugiperda Larvae |
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| LEI Chengfeng,JIANG Ganming,PENG Ling,YANG Jian,HU Jia,SUN Xiulian.Identification of an Isolate of Ostrinia furnacalis Nucleopolyhedrovirus and Its Bioactivity against Spodoptera frugiperda Larvae[J].Chinese Journal of Biological Control,2019,35(5):741-746. |
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| Authors: | LEI Chengfeng JIANG Ganming PENG Ling YANG Jian HU Jia SUN Xiulian |
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| Affiliation: | 1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China;2. Huanggang Plant Protection and Quarantine Station, Huanggang 438000, China;3. Wuhan Institute of Vegetable Science, Wuhan Academy of Agricultural Sciences, Wuhan 430345, China |
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| Abstract: | In this study, we conducted molecular identification, morphological characterisation and bioactivity of a new nucleopolyhedrovirus with wide host range isolated from Ostrinia furnacalis (Guenée) in Enshi, Hubei Province. The results indicated that the newly isolated nucleopolyhedrovirus was homologous to Rachiplusia ou nucleopolyhedrovirus and Anagrapha falcifera nucleopolyhedrovirus, while Kimura 2-parameter distances did not support it was the same species as these two viruses. The median lethal concentration of Ostrinia furnacalis nucleopolyhedrovirus was 3.86 times of that of Mamestra brassicae nucleopolyhedrovirus. These results will lead to the development of efficacious virus-based bioinsecticides against Spodoptera frugiperda. |
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| Keywords: | nucleopolyhedrovirus Spodoptera frugiperda bioactivity |
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