| 生物炭对Bt Cry1Ac蛋白抗紫外能力影响 |
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| 引用本文： | 饶文华,占亚婷,方云,游雨欣,聂丹玥,郭雪萍,张顶洋,关雄,潘晓鸿.生物炭对Bt Cry1Ac蛋白抗紫外能力影响[J].中国生物防治学报,2020,36(5):714-720. |
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| 作者姓名： | 饶文华 占亚婷 方云 游雨欣 聂丹玥 郭雪萍 张顶洋 关雄 潘晓鸿 |
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| 作者单位： | 福建农林大学闽台作物有害生物生态防控国家重点实验室/生物农药与化学生物学教育部重点实验室, 福州 350002 |
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| 基金项目： | 国家重点研发计划（2017YFE0121700）；福建农林大学校杰出青年项目（xjq201719）；福建农林大学科技发展资金（CXZX2019005S，KHF190013，KF2015064-065） |
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| 摘 要： | 为了研究生物炭对紫外线的防护作用，以豆壳烧制的生物炭作为载体，研究生物炭对Bt Cry1Ac蛋白的吸附行为以及生物炭对Cry1Ac蛋白的紫外保护作用。使用扫描电子显微镜、透射电子显微镜、X-射线粉末衍射以及傅立叶红外光谱等手段对生物炭的形貌和结构进行表征。结果表明，生物炭是典型的多孔结构材料，表面具有丰富的官能团。Cry1Ac蛋白与生物炭吸附平衡时间为50 min，最合适的吸附浓度比（生物炭：蛋白）为1：100，二者吸附符合准二级动力学模型和Langmuir模型。在UVB紫外照射4 h后，生物炭与Cry1Ac蛋白复合物对棉铃虫的生物活性是单纯蛋白的4.93倍，显示生物炭具有较好的紫外抵抗效果。研究结果初步表明，制备得到的生物炭能够显著提高Cry1Ac蛋白的抗紫外能力，为后续研发耐受紫外线的农药剂型提供新材料。
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| 关 键 词： | 苏云金芽胞杆菌 Cry1Ac蛋白 生物炭 吸附 抗紫外 |
| 收稿时间： | 2020-06-02 |
The Effect of Biochar on Anti-ultraviolet Ability of Cry1Ac Protein |
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| RAO Wenhua,ZHAN Yating,FANG Yun,YOU Yuxin,NIE Danyue,GUO Xueping,ZHANG Dingyang,GUAN Xiong,PAN Xiaohong.The Effect of Biochar on Anti-ultraviolet Ability of Cry1Ac Protein[J].Chinese Journal of Biological Control,2020,36(5):714-720. |
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| Authors: | RAO Wenhua ZHAN Yating FANG Yun YOU Yuxin NIE Danyue GUO Xueping ZHANG Dingyang GUAN Xiong PAN Xiaohong |
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| Affiliation: | State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University/Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China |
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| Abstract: | In order to study the protective effect of biochar on ultraviolet, we made biochar from bean shell as the carrier, and the adsorption behavior of biochar on Bt Cry1Ac protein and the UV protection of biochar on Cry1Ac protein were studied. The morphology and structure of biochar were characterized by SEM, TEM, XRD and FTIR. The results showed that biochar is a typical porous material with abundant functional groups on its surface. The adsorption equilibrium time of Cry1Ac protein and biochar was 50 min, and the most suitable adsorption concentration ratio (biochar:protein) was 1:100. The adsorption of Cry1Ac protein and biochar conformed to the quasi second-order kinetic model and Langmuir model. After UV irradiation for 4 hours, the biological activity of biochar and Cry1Ac protein complex on Helicoverpa armigera was 4.93 times higher than that of pure protein, which showed that biochar had better UV resistance ability. The results show that biochar could significantly improve the anti-UV ability of Cry1Ac protein, which provide new materials for development of UV resistant pesticide formulation. |
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| Keywords: | Bacillus thuringiensis Cry1Ac protein biochar adsorption UV resistance |
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