| 木霉菌对黄瓜幼苗生长和膜脂过氧化指标的影响及对枯萎病的防治效果 |
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| 引用本文： | 高长敏,马光恕,廉华,刘明鑫,张春秋,曲虹云.木霉菌对黄瓜幼苗生长和膜脂过氧化指标的影响及对枯萎病的防治效果[J].中国生物防治学报,2018,34(5):762-770. |
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| 作者姓名： | 高长敏 马光恕 廉华 刘明鑫 张春秋 曲虹云 |
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| 作者单位： | 1. 黑龙江八一农垦大学农学院, 大庆 163319;2. 黑龙江省农业科学院园艺分院, 哈尔滨 150069 |
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| 基金项目： | 黑龙江省自然科学基金（QC2016035）；黑龙江省农垦总局科技项目（HNK125B-02-12）；黑龙江省学位与研究生教育教学改革研究研究项目（JGXM_HLJ_2015117） |
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| 摘 要： | 本文开展了3种生防木霉菌，包括棘孢木霉Trichoderma asperellum 525、哈茨木霉T.harzianum 610和拟康氏木霉T.pseudokoningii 886防治黄瓜枯萎病的盆栽试验，研究这3种木霉菌对黄瓜幼苗生长、膜脂过氧化指标的影响及对黄瓜枯萎病的防治效果。结果表明，木霉菌对黄瓜枯萎病的田间防治效果均达到78%以上，且以棘孢木霉525的田间防治效果最高，达到81.53%。与只接种枯萎病病原菌的对照相比，3株木霉菌单独接种或与黄瓜枯萎病病原菌同时接种均可以显著提高黄瓜幼苗株高、茎粗、叶面积、根体积、地上部鲜重、地下部鲜重，显著提高黄瓜叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、多酚氧化酶（PPO）活性，显著降低质膜透性和丙二醛（MDA）含量，其中以拟康氏木霉886单独接种促进效果最强。研究表明，3种木霉菌通过促进黄瓜幼苗生长，增强植物抗氧化酶活性，降低质膜透性和丙二醛含量，从而提高对黄瓜枯萎病的抗性。
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| 关 键 词： | 木霉菌 黄瓜枯萎病 幼苗生长 膜脂过氧化 |
| 收稿时间： | 2018-02-01 |
Effect of Trichoderma on the Growth of Cucumber Seedlings,Membrane Lipid Indexes and Control Effect against Fusarium Wilt |
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| GAO Changmin,MA Guangshu,LIAN Hua,LIU mingxin,ZHANG Chunqiu,QU Hongyun.Effect of Trichoderma on the Growth of Cucumber Seedlings,Membrane Lipid Indexes and Control Effect against Fusarium Wilt[J].Chinese Journal of Biological Control,2018,34(5):762-770. |
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| Authors: | GAO Changmin MA Guangshu LIAN Hua LIU mingxin ZHANG Chunqiu QU Hongyun |
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| Affiliation: | 1. College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China;2. Heilongjiang Academy of Agricultural Sciences Horticulture Branch, Harbin 150069, China |
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| Abstract: | Cucumber Fusarium wilt is one of the major destructive soil-borne disease in the cucumber production, and the application of biocontrol fungi to control cucumber wilt is of great significance to the safe production of cucumber. To clarify the effect of biocontrol Trichoderma on the growth of cucumber seedlings, membrane lipid peroxidation and the control effect against cucumber Fusarium wilt, three antagonistic fungi against pathogen of cucumber Fusarium wilt, T. asperellum 525, T. harzianum 610 and T. pseudokoningii 886, were used to carry out pot experiment in this paper. The results showed that the field control effect of three Trichoderma strains against cucumber Fusarium wilt were more than 78%, and the field control effect of T. asperellum 525 was the highest, reaching 81.53%. Compared with the treatment with the cucumber Fusarium Wilt pathogen alone, all those treatments with Trichoderma alone and simultaneous inoculation with Trichoderma and pathogen significantly improved cucumber plant height, stem diameter, leaf area, root volume, above ground fresh weight and underground fresh weight of cucumber, increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and polyphenol oxidase (PPO) activity, and decreased membrane permeability and malondialdehyde (MDA) content. The results indicated that the three Trichoderma strains have the function of controlling cucumber Fusarium wilt and promoting growth of cucumber. Trichoderma can induce cucumber resistance to Fusarium wilt by promoting cucumber seedling growth, increasing plant antioxidant enzyme activity and reducing plasma membrane permeability and malondialdehyde content. |
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| Keywords: | Trichoderma cucumber Fusarium wilt seedlings growth membrane lipid peroxidation |
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