| 拮抗性木霉菌株抗逆性筛选评价标准与方法 |
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| 引用本文： | 王强强,窦恺,陈捷,蒋细良,杨合同,王伟.拮抗性木霉菌株抗逆性筛选评价标准与方法[J].中国生物防治学报,2019,35(1):99-111. |
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| 作者姓名： | 王强强 窦恺 陈捷 蒋细良 杨合同 王伟 |
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| 作者单位： | 1. 上海交通大学农业与生物学院/国家微生物代谢重点实验室/农业部都市农业(南方)重点实验室, 上海 200240;2. 中国农业科学院植物保护所, 北京 100193;3. 山东省科学院生态研究所, 济南 250014;4. 华东理工大学生物工程学院/生物反应器工程国家重点实验室, 上海 200237 |
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| 基金项目： | 国家重点研发计划（2017YFD0201108）；国家科技基础性工作专项（2014FY120900）；国家现代农业产业技术体系（CARS-02） |
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| 摘 要： | 本研究旨在为高效筛选耐受高温胁迫、低温胁迫、盐胁迫及干燥（干旱）胁迫拮抗性木霉菌株提供筛选阈值。根据5种、20株拮抗尖孢镰孢菌效果较好的木霉菌对上述逆境因子的反应差异，建立每种逆境因子在不同水平下的木霉菌株生长和酶活变化检测阈值和标准化评价方式；分析木霉菌在不同胁迫条件下发育相关生化因子与菌株生长变化之间的关系，及相应胁迫下的孢子萌发率与菌落直径之间的相关性。通过正态性检验获得供试木霉菌株生长响应胁迫的阈值为：1 mol/L NaCl（盐胁迫阈值），36℃（高温胁迫阈值），14℃（低温胁迫阈值），400 g/L PEG-6000（干旱胁迫阈值）。相应胁迫下的菌落直径与孢子萌发率明显正相关。菌株在大部分胁迫下的抗坏血酸过氧化物酶（APX）及过氧化氢酶（CAT）酶活与菌丝干重有明显的相关性。本研究为科学评价生物防治木霉菌资源的抗逆性提供了依据。
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| 关 键 词： | 木霉菌 耐受性 评价阈值 |
| 收稿时间： | 2017-11-18 |
The Standards and Methods for Efficient Evaluation of Tolerance of Antagonistic Trichodema Strains |
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| WANG Qianqiang,DOU Kai,CHEN Jie,JIANG Xiliang,YANG Hetong,WANG Wei.The Standards and Methods for Efficient Evaluation of Tolerance of Antagonistic Trichodema Strains[J].Chinese Journal of Biological Control,2019,35(1):99-111. |
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| Authors: | WANG Qianqiang DOU Kai CHEN Jie JIANG Xiliang YANG Hetong WANG Wei |
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| Abstract: | Efficient screening of Trichoderma strains with biocontrol potential and tolerance to various environment stress factors including extreme temperature, salt and drought etc was extensively required. Hence in this work, we examined a total of 20 Trichoderma isolates from five species in the tolerance performance to different stress conditions, including high and/or low temperature, salt and drought, and then established the threshold and standardized evaluation method based on colony growth and selected biochemical factors activity/contents of Trichoderma strains at different levels of each stress factor. Eventually the correlation of colony growth changes of Trichoderma spp. with the selected enzymes activity or MDA contents was analyzed under different stress conditions, and the correlation between spore germination rate and colony diameter under the corresponding stress was also analyzed. These results showed that the thresholds for Trichoderma growth response to stress factors were 1 mol/L of NaCl (salt stress), 36℃ (high temperature), 14℃ (low temperature) and 400 g/L PEG-6000 (drought stress). The diameter of colony was positively correlated with the spore germination rate under the corresponding stress. Most of the selected biochemical factors activity indexes under different stresses factors were significantly correlated with the dry weight of mycelium. In conclusion, the establishment of threshold of Trichoderma strains resistant to different stresses factors provided the essential basis for scientific evaluation of Trichoderma, which is important for evaluation and utilization of tolerant Trichoderma resources as biocontrol agents. |
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| Keywords: | Trichoderma tolerance evaluation threshold |
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