Effects of increasing protein level on the performance,enzyme activity and body composition of the Brazilian sardine,Sardinella brasiliensis (Steindachner, 1879) |
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| Authors: | FC Sterzelecki JK Sugai M Baloi G Passini CVA de Carvalho DM Fracalossi VR Cerqueira |
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| Affiliation: | 1. Departamento de aquicultura, Laboratório de Piscicultura Marinha (LAPMAR), Centro de Ciências Agrárias, UFSC, Florianópolis, SC, Brasil;2. Departamento de Bioquímica, Laboratório de Enzimologia Aplicada, Centro de Ciências Biológicas, UFSC, Florianópolis, SC, Brasil;3. Departamento de aquicultura, Laboratório de Nutri??o de Espécies Aquícolas (LABNUTRI), Centro de Ciências Agrárias, UFSC, Florianópolis, SC, Brasil |
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| Abstract: | A six‐week growth trial was performed to estimate the dietary protein requirements for maximum growth of juvenile Brazilian sardine (Sardinella brasiliensis) based on growth performance, feed utilization, body composition and digestive enzyme activity. Six isoenergetic diets were formulated to contain protein levels that increased from 250 to 500 g/kg. Each diet was randomly assigned to triplicate groups of 160 fish with mean initial body weight of 0.93 ± 0.13 g, which were fed four times a day to apparent satiation. Growth tended to increase with the increase in the dietary protein level up to 400 g/kg. Total protein intake was indirectly correlated to apparent protein utilization. No significant differences in whole‐body composition were found between fish fed the different protein levels. Acid protease and neutral lipase activities did not show significant differences among the different protein dietary groups. Alkaline protease activity increased in fish fed up to 350 g/kg of protein and amylase activity in fish fed up to 400 g/kg. Using polynomial regression, 367 g/kg was estimated to be the optimum dietary protein requirement for maximum weight gain of juvenile Brazilian sardines. |
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| Keywords: | brazilian sardine digestive enzymes feed protein requirement Sardinella brasiliensis |
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