| 角叉菜凝集素的分离纯化及其性质 |
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| 引用本文： | 李丹彤,崔铁军,马国庆,汤霞,李熙宜.角叉菜凝集素的分离纯化及其性质[J].中国水产科学,2000,7(3):80-84. |
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| 作者姓名： | 李丹彤 崔铁军 马国庆 汤霞 李熙宜 |
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| 作者单位： | 大连水产学院,养殖系,辽宁,大连,116023 |
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| 基金项目： | 农业部八五一青年基金资助项目(渔85-93-青05) |
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| 摘 要： | 角叉菜（Ｃｈｏｎｄｒｕｓ ｏｃｅｌｌａｔｕｓ）经磷酸盐缓冲液抽提，２０％～７５％硫酸铵分级沉淀，牛甲状腺球蛋白－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析，可以从红藻角叉菜中纯化出角叉菜凝集素（ＣＯＬ），在ＰＡＧＥ上显示单一蛋白染色带，在等电聚焦电泳上显示单一蛋白染色带，其ＰＩ为８．３０，纯化后的ＣＯＬ的最大此外吸收峰在２８５ｎｍ，用ＳｅｐｈａｄｅｘＧ－２００分子筛层析测得其分子量为４２５２。该凝集素可以凝集
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| 关 键 词： | 角叉菜 凝集素 纯化 理化性质 |
| 文章编号： | 1005-8737(2000)03-0080-05 |
| 修稿时间： | 2000-01-26 |
Isolation and purification of lectin from Chondrus ocellatus and the lectin properties |
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| LI Dan-tong,CUI Tie-jun,MA Guo-qing,TANG Xia,LI Xi-yi.Isolation and purification of lectin from Chondrus ocellatus and the lectin properties[J].Journal of Fishery Sciences of China,2000,7(3):80-84. |
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| Authors: | LI Dan-tong CUI Tie-jun MA Guo-qing TANG Xia LI Xi-yi |
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| Affiliation: | LI Dan-tong
,CUI Tie-jun
,MA Guo-qing
,TANG Xia
,LI Xi-yi
(Department of Aquaculture, Dalian Fisheries College, Dalian 116023, China) |
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| Abstract: | The lectin from red algae Chondrus ocellatus (COL) was purified by extraction with PBS, followed by 20% - 75% ammonium sulfate fractionation and an affinity chromatography on the Bovine- thyroglobulinSepharose 4B column. One single band appeared on both PAGE and isoelectrofocusing electrophoretogram of the lectin. Its isoelectric Point was 8. 30, with peak abSOrption at 285 nm. Its molecular weight was 4 252 on sephadexG-200. The COL was nonspecific in agglutination for any type of human erythrocytes, and the hemagglutinating activities were all at the same level. The agglutination for rabbit was the highest among erythrocytes of human(A, B, AB, O), rabbit, common carp and crucian carp. The hemagglutinating activity for rabbit could not be inhibited by D-fructose, D-mannose, D-galactose, glucose, sucrose, mannan, gamma- globulin (human ) and egg albumin, but inhibited by bovine-thyroglobulin, and the minimum inhibitory concentration was 3. 10 mg/ ml. |
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| Keywords: | Chondrus ocellatus lectin purification phsico-ehemical properties |
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