| 黄条鰤线粒体全基因组测序及结构特征分析 |
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| 引用本文： | 史宝,柳学周,刘永山,张言祥,高全义,徐永江,王滨,姜燕,宋雪松.黄条鰤线粒体全基因组测序及结构特征分析[J].中国水产科学,2019,26(3):405-415. |
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| 作者姓名： | 史宝 柳学周 刘永山 张言祥 高全义 徐永江 王滨 姜燕 宋雪松 |
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| 作者单位： | 1. 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室, 中国水产科学研究院黄海水产研究所, 山东 青岛 266071;2. 上海海洋大学水产与生命学院, 上海 201306;3. 大连富谷水产有限公司, 辽宁 大连 116400 |
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| 基金项目： | 现代农业产业技术体系专项经费资助项目（CARS-47）；中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金（2018GH17）；青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室开放课题（2017-3A01）；青岛市民生科技计划项目（17-3-3-61-nsh）；中国水产科学研究院黄海水产研究所基本科研业务费项目（20603022017016）；国家自然科学基金项目（31772829）. |
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| 摘 要： | 通过二代测序、软件拼接获得中国黄海海域黄条鰤(Seriola aureovittata)线粒体基因组全序列。其序列全长为16609 bp,碱基组成分别为A(26.68%)、G(17.84%)、C(30.12%)和T(25.36%);共有13条蛋白编码基因, 22个tRNA基因, 2个rRNA基因,除NAD6、trnQ、trnA、trnN、trnC、trnY、trnS、trnE、trnP外,其余基因均在H链上编码。黄条鰤线粒体基因组全序列与蛋白编码基因的A+T含量分别为52.05%和51.085%,具有明显的AT偏好性。线粒体基因中存在2个散在重复序列,分别位于NAD1基因序列正义链的中游和COX2基因序列反义链的上游。在其22个tRNA基因中,除了tRNAGly外,均具有典型的三叶草二级结构。黄条鰤线粒体基因组的蛋白编码基因起止位点与密码子除COX1、NAD5外,均与日本海域出产的黄条鰤(Seriola lalandi)完全吻合,且COX1、NAD5基因皆短于日本黄条鰤;两者间存在一定的遗传差异。基于18种隶属于13属的鲹科鱼类线粒体基因组全序列构建系统发生树,可知小甘鲹(Seriolina nigrofasciata)、黄条鰤、五条鰤(Seriola quinqueradiata)、高体鰤(Seriola dumerili)、长鳍鰤(Seriola rivoliana)同属一近支,且黄条鰤与五条鰤亲缘关系最近,与小甘鲹进化距离较远。
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| 关 键 词： | 黄条鰤 线粒体基因组 序列分析 系统进化 |
| 修稿时间： | 2019/5/16 0:00:00 |
Complete sequence and gene organization of the mitochondrial genome of Seriola aureovittata |
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| SHI Bao,LIU Xuezhou,LIU Yongshan,ZHANG Yanxiang,GAO Quanyi,XU Yongjiang,WANG Bin,JIANG Yan,SONG Xuesong.Complete sequence and gene organization of the mitochondrial genome of Seriola aureovittata[J].Journal of Fishery Sciences of China,2019,26(3):405-415. |
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| Authors: | SHI Bao LIU Xuezhou LIU Yongshan ZHANG Yanxiang GAO Quanyi XU Yongjiang WANG Bin JIANG Yan SONG Xuesong |
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| Affiliation: | 1. Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology(Qingdao);Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China;3. Dalian Fugu Fishery Co., Ltd, Dalian 116400, China |
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| Abstract: | Seriola aureovittata is a large marine pelagic predatory fish species in the global warm temperate ocean, which is mainly distributed near the offshore rocks and reefs of the Bohai Sea and Yellow Sea of China. In recent years, most research has focused on its breeding and breeding technology at home and abroad, whereas few studies have been conducted on its genome and group composition. The complete mitochondrial DNA was extracted from the somatic side muscles of S. aureovittata. The complete mitochondrial genome of S. aureovittata was obtained using second generation sequencing and splicing with software of DNAStar. The sequence length was 16609 bp, and the base composition was A (26.68%), G (17.84%), C (30.12%), and T (25.36%). It contained 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes. Except for NAD6, trnQ, trnA, trnN, trnC, trnY, trnS, trnE, and trnP, all the other genes were encoded on the heavy strand (H strand). The A+T content of the complete mitochondrial genome sequence and protein-coding genes were 52.05% and 51.085%, respectively. There was a strong bias toward A+T in the genome of S. aureovittata. There were two repeated sequences scattered in the genes. One was located in the middle part of the sense strand of the NAD1 gene sequence, and the other was located in the upper part of the antisense strand of COX2 gene sequence. All tRNA genes had typical cloverleaf structure except tRNAGly. Its start-stop sites and codons of protein-coding genes, except COX1 and NAD5, were the same as that of Seriola lalandi, which inhabits in the oceans of Japan. The COX1 and NAD5 protein genes of S. aureovittata were shorter than those of S. lalandi. It was preliminarily assumed that there were genetic differences between S. aureovittata and S. lalandi. The NJ-tree based on the complete mitochondrial DNA genomes showed the evolutionary position of S. aureovittata relative to that of 18 other Carangidae species. The results showed that the Seriola spp. belong to a secondary tree with Seriolina nigrofasciata. Furthermore, S. aureovittata and Seriolina quinqueradiata had a closer relationship than others in the tree, whereas S. aureovittata had the most distant relationship with S. nigrofasciata. This study provides basic data for solving different geographical population and kinship relations of S. aureovittata. |
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| Keywords: | Seriola aureovittata mitochondrial genome sequence analysis phylogenetic tree |
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