| 卵形鲳!线粒体１６ＳｒＲＮＡ基因全长序列的克隆与分析 |
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| 引用本文： | 彭金霞,彭敏,陈秀荔,蒋伟明,杨春玲,李咏梅.卵形鲳!线粒体１６ＳｒＲＮＡ基因全长序列的克隆与分析[J].水生态学杂志,2010,31(4):81-85. |
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| 作者姓名： | 彭金霞 彭敏 陈秀荔 蒋伟明 杨春玲 李咏梅 |
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| 作者单位： | 广西水产研究所,广西,南宁,530021 |
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| 基金项目： | 国家科技支撑计划农业领域课题(编号2007BAD29B03) |
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| 摘 要： | 根据近源物种线粒体序列的同源比对，在16S rRNA基因上下游保守区域设计一对通用引物。PCR扩增获得特异的DNA片段，经克隆、测序和比对证实该片段包含了卵形鲳鲹线粒体16S rRNA全长序列1725bp。对5个个体分别测序后比对，发现卵形鲳鲹16S rRNA基因在钦州湾种群个体间存在至少7个变异位点，使5个个体分别具有5种不同的单倍型。将卵形鲳鲹和鲹科其它物种的16S rRNA序列进行比，根据比对结果构建的鲳鲹科各物种的系统进化树，支持鲹科下设四个亚科(鲹亚科，鰤亚科，鲳鲹亚科，鰆鲹亚科)的分类系统。综上所述，16S rRNA基因既可用于卵形鲳鲹种群遗传多样性分析，又适用于鲹科鱼类的系统进化分析。
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| 关 键 词： | 卵形鲳鲹 线粒体 16S rRNA基因 种质资源 系统发育进化树 |
| 收稿时间： | 2/9/2010 6:13:16 PM |
| 修稿时间： | 5/22/2010 8:05:52 PM |
Cloning and Sequence Analysis of Trachinotus ovatus Mitochondrial 16S rRNA Gene |
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| pengjinxi,pengmin,chenxiuli,jiangweiming,yangchunling and liyongmei.Cloning and Sequence Analysis of Trachinotus ovatus Mitochondrial 16S rRNA Gene[J].Journal of Hydroecology,2010,31(4):81-85. |
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| Authors: | pengjinxi pengmin chenxiuli jiangweiming yangchunling and liyongmei |
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| Affiliation: | Guangxi Fisheries Research Institute,Guangxi Fisheries Research Institute,Guangxi Fisheries Research Institute,Guangxi Fisheries Research Institute,Guangxi Fisheries Research Institute,Guangxi Fisheries Research Institute |
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| Abstract: | Based on multiple alignment of mitochondrial sequence from neighbored spices, a pair of conserved primers was designed on the flanking region of 16S rRNA gene. The PCR amplification product was cloned to PGEM-Teasy vector and sequenced. Blast on NCBI confirmed that the sequence contain whole 1725bp of 16S rRNA gene. Alignment of sequence from 5 fishes of the same population revealed 7 variation sites, which form 5 different genotypes. Based on the multiple alignment of 16S rRNA sequence of Trachinotus ovatus and other Carangidae species, a phylogenetic tree was constructed which supporting the idea that Carangidae could be divided into four subfamilies: Caranginae, Seriolinae, Trachinotinae and Chorineminae. So, 16S rRNA gene is a useful tool which can be used not only in genetic polymorphism analysis of Trachinotus ovatus, but also in phylogenetic analysis of Caranginae species. |
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| Keywords: | Trachinotus ovatus mitochondrial 16S rRNA gene genetic resource phylogenetic tree |
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