| 对虾白斑综合征病毒在螯虾动物模型的感染特性 |
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| 引用本文： | 朱建中.对虾白斑综合征病毒在螯虾动物模型的感染特性[J].水产学报,2001,25(1):47-51. |
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| 作者姓名： | 朱建中 |
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| 作者单位： | [1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室 [2]南京农业大学农业部动物疫病诊 |
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| 摘 要： | 应用克氏原螯虾作为动物模型研究对虾白斑综合征病毒（ＷＳＳＶ）的感染增殖特性，涉及感染温度，感染途径，继发感染，半数致死量（ＬＤ５０），免疫保护及保存期等，结果显示，２２－２５℃时，接种ＷＳＳＶ的螯虾一般于２－７d内死亡，温度升高对病毒增殖影响不显著，３０－３２℃时，平均死亡时间２－６d，温度降低对病毒增殖影响较显著，１５－１９℃时，接种螯虾平均２－１０d内死亡，８－１０℃时，平均死亡时间３－６d，感染途径分别用腹节肌肉，腹节皮下洲射及口服，均能使螯虾感染发病，而浸泡方式不能使螯虾发病，细菌分离和细菌定量结果表明，寄考于螯虾心脏，肝胰腺内的阴沟肠杆菌在感染后期大量增殖，菌量分别是正常螯虾的２５和３０倍，形成继发感染，用螯虾心脏，肝胰腺内的阴肠杆菌在感染后期大量增殖，菌量分别是政党螯虾的２５和３０倍，形成继发梁。用螯虾测定ＷＳＳＶ的ＬＤ５０为１０^-6.5.mL^-1种毒液，将病毒５６℃，３０min灭活后免疫螯虾，不能使螯虾形成免疫保护，ＷＳＳＶ匀浆液－３０℃冻存１年后失活，而－３０℃冻存于螯虾体内ＷＳＳＶ保存１年后仍有活力。
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| 关 键 词： | 克氏原螯虾 白斑综合征病毒 感染温度 感染途径 继发感染 免疫保护 保存期 |
| 文章编号： | 1000-0615(2001)01-0047-05 |
| 收稿时间： | 2014/4/16 0:00:00 |
| 修稿时间： | 2000年1月5日 |
Characterization of shrimp white spot syndrome virus(WSSV) infection in Cambarus proclarkii |
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| ZHU Jian zhong.Characterization of shrimp white spot syndrome virus(WSSV) infection in Cambarus proclarkii[J].Journal of Fisheries of China,2001,25(1):47-51. |
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| Authors: | ZHU Jian zhong |
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| Affiliation: | Key Lab of Animal Disease Diagnostic and Immunology, The Ministry of Agriculture Nanjing Agriculture University, Nanjing 210095,China |
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| Abstract: | Crawf ishes ( Cambarus proclarkii ) as animal were infected with white spot syndrome virus (WSSV) for
studying various infection characters, including temperature, transfer route, successive infection, median lethal
dose ( LD50) , immunity against virus and virus conservation etc. The results showed crawf ishes usually died in 2
- 7d after infection at the temperature of 22- 25 .. , in 2- 10d at 15- 19 .. , in 3- 13d at 8- 10 .. and 2- 6d at
30- 32 .. . Below 5 .. , the infected crawf ishes could remain active for more than one month. By injecting and
oral feeding, WSSV could infected crawfishes successfully and there was no significant difference between muscle
injection and hypoderm injection. Soaking with WSSV failed to infect crawfishes. Bacteria examination in
healthy and diseased crawfishes showed Enterobacter cloacae in the heart and hepatopancreas multiplied 25 and 30
times respectively , indicating that bacteria successive infect ion existed during the virus infection. The LD50 was
10
- 6. 5..mL
- 1
WSSV. Crawfishes which were immunized with WSSV inactivated at 56 .. for 30min could not
obtain immunity protection from. WSSV f rom homogenate stored at - 30 .. for 1 year could not be recovered but
f rom crawfishes stored at - 30 .. for 1 year could be recovered successfully. |
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| Keywords: | Cambarus proclarkii white spot syndrome virus(WSSV) infection temperature infection route |
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