首页 | 官方网站   微博 | 高级检索  
     

泥蚶受精过程的细胞学荧光显微观察
引用本文:孙慧玲.泥蚶受精过程的细胞学荧光显微观察[J].水产学报,2000,24(2):104-107.
作者姓名:孙慧玲
作者单位:1. 中国水产科学研究院黄海水产研究所,青岛,266071
2. 青岛海洋大学测试中心,266003
基金项目:国家攀登B计划资助项目! (海水增养殖生物优良种质和抗病力的基础研究 ),PDB -6-3 -2号
摘    要:采用荧光染色剂DAPI已固定的泥蚶成熟卵和受精卵进色染色的方法,在显微镜下观察泥蚶受精细胞学过程。通过DAPI的染色,在荧光显微镜下可清楚观察到,泥蚶的成熟未受精卵处于第一次成熟分裂的中期,精子入卵后分裂再次启动,释放出第一和第二极体,完成成熟分裂,部分受精卵的第一极体有极明显的分裂现象,精子入卵的部位是随机的,且自精子入卵至成熟分裂完成,入卵的精子在形态等方面将发生一系列变化,形成雄性原核。雌雄

关 键 词:泥蚶  受精过程  细胞学  荧光染色显微观察
收稿时间:2014/4/17 0:00:00
修稿时间:1999-02-08

Cytological observation on fertilization of Tegillarca granosa with fluorescent microscope
Sun Huiling.Cytological observation on fertilization of Tegillarca granosa with fluorescent microscope[J].Journal of Fisheries of China,2000,24(2):104-107.
Authors:Sun Huiling
Affiliation:Yellow Sea Fisheries Research Institute ,CAFS,Qingdao 266071
Abstract:The fertilzed eggs of Tegillarca granosa stained with DAPI were observed by fiuorescent microscope. The result shows that the mature eggs were at metaphase of first meiosis.After sperm penetrating,the eggs released their first and second polar bodies and finished their meiotic division.Some first polar bodies divided into two small cells.The eggs Tegillarca granosa became monospermic. While the male and the female pronucleus were produced,they expanded to their maximal size.And the male pronucleus moved to the female pronucleus during the period of associating of the two pronucleus.
Keywords:Tegillarca granosa    fertilization  fluorescent microscope
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《水产学报》浏览原始摘要信息
点击此处可从《水产学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号