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| 两种黄盖鲽线粒体DNA部分片段比较分析 | |
| 引用本文： | 张岩,肖永双,高天翔,于函.两种黄盖鲽线粒体DNA部分片段比较分析[J].水产学报,2009,33(2):201-207. |
| 作者姓名： | 张岩 肖永双 高天翔 于函 |
| 作者单位： | 1. 中国海洋大学海水养殖教育部重点实验室,山东,青岛,266003;中国水产科学研究院黄海水产研究所,山东,青岛,266071 2. 中国海洋大学海水养殖教育部重点实验室,山东,青岛,266003 3. 辽宁师范大学生命科学学院,辽宁,大连,116029 |
| 基金项目： | 国家高技术研究发展计划,国家重点基础研究发展计划 |
| 摘 要： | 比较分析了钝吻黄盖鲽（Pleuronectes yokohama）和尖吻黄盖鲽（P. herzensteini）线粒体基因组COI、Cyt b基因以及D-Loop片段总长度为1373 bp的核苷酸序列,两种间共检测到107处核苷酸替换，蛋白质编码基因上的核苷酸替代主要是第三密码子位点上的同义替换。核苷酸组成分析结果表明：三个目的片段的鸟嘌呤（G）含量普遍较低，在两个蛋白编码基因第三密码子位点上表现得尤为明显。两种黄盖鲽在线粒体基因组不同片段上存在明显的遗传分化，核苷酸替代速率最快的是D-Loop，COI和Cyt b核苷酸替代速率基本一致。建议在进行黄盖鲽属鱼类分子系统发育研究时，应该针对不同研究目的选择合适的分子标记。基于Cyt b基因片段序列的分析结果表明：钝吻黄盖鲽和尖吻黄盖鲽两种的分岐时间约为200万年，两种间的分化事件发生在更新世（Pleistocene）。 |
| 关 键 词： | 钝吻黄盖鲽 尖吻黄盖鲽 线粒体DNA 核苷酸组成 分歧时间 |
| 收稿时间： | 8/8/2008 12:00:00 AM |
| 修稿时间： | 2008/9/28 0:00:00 |
Comparative analysis of mtDNA gene sequences between two species of Pleuronectes |
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| ZHANG Yan,XIAO Yong-shuang,GAO Tian-xiang and YU Han.Comparative analysis of mtDNA gene sequences between two species of Pleuronectes[J].Journal of Fisheries of China,2009,33(2):201-207. | |
| Authors: | ZHANG Yan XIAO Yong-shuang GAO Tian-xiang and YU Han |
| Affiliation: | yellow Sea Fisheries Research Institute,Ocean University of China,Ocean University of China, |
| Abstract: | Partial nucleotide sequences of mitochondrial DNA COI, Cytb genes and D-Loop were sequenced determined and analyzed for Pleuronectes yokohama and P. herzensteini. 107 nucleotide substitutions were checked between the two species in the total 1373 bp sequences analyzed, most of them were synonymous transitions at the third codon positions in the two protein-coding genes. Nucleotide composition analysis indicated a strong bias against guanine (G) in the three fragments, especially in the third codon positions of COI and Cytb gene. There was obvious difference on the degree of genetic divergence between the two species in the three mtDNA segments. The substitution rate for D-Loop was the highest and the rates for COI and Cytb were generally similar. It is suggested that gene markers used in future research should be selected carefully according to the different situations. The estimated divergence time between the two Pleuronectes species based on Cytb gene was about 2 million years, which indicated a Pleistocene divergence for the two species. |
| Keywords: | Pleuronectes yokohama P herzensteini mtDNA nucleotide composition divergence rate |
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