| 基于mtDNACytb基因序列的我国不同水系野生鲇种群遗传多样性与种群历史分析 |
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| 引用本文： | 徐丹丹,黄燕,曾庆,李斌,彭作刚.基于mtDNACytb基因序列的我国不同水系野生鲇种群遗传多样性与种群历史分析[J].水产学报,2017,41(10):1489-1499. |
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| 作者姓名： | 徐丹丹 黄燕 曾庆 李斌 彭作刚 |
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| 作者单位： | 1. 内江师范学院生命科学学院,长江上游鱼类资源保护与利用四川省重点实验室,四川内江 641100;2. 西南大学淡水鱼类资源与生殖发育教育部重点实验室,重庆,400715 |
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| 基金项目： | 公益性行业（农业）科研专项（200903048-089）；内江师范学院校级项目（15JC12） |
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| 摘 要： | 为进一步了解鲇这一广布种的遗传多样性与种群遗传结构,阐明该经济鱼类的种群历史动态,本研究对我国长江(上游、中游)、珠江、福建、海南、辽河地区的野生鲇样本进行mtDNA Cytb基因序列测定与种群遗传多样性和遗传结构分析。结果显示,在所分析的216个序列样本中,共检测出78个单倍型,其中Hap_45在鲇不同群体中分布最为广泛;鲇总群体具有较高的单倍型多样性(Hd=0.948±0.009)和核酸多样性(Pi=0.017 99±0.000 55),暗示了其自然种群数量的相对稳定;单倍型系统发育与网络关系图分析显示,78种单倍型被分为4个分支,且不同单倍型分布相对集中,划分为4个谱系。中性检验与错配分析表明,不同水系野生鲇种群(4个谱系)在约晚更新世时期(0.04~0.05百万年)经历过近期种群扩张事件。
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| 关 键 词： | 鲇 细胞色素b(Cytb) 线粒体DNA 种群历史 |
| 收稿时间： | 2016/10/11 0:00:00 |
| 修稿时间： | 2017/1/7 0:00:00 |
Genetic diversity and population history among geographic populations of Silurus asotus in different water systems in China based on mtDNA Cytb gene sequences |
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| XU Dandan,HUANG Yan,ZENG Qing,LI Bin and PENG Zuogang.Genetic diversity and population history among geographic populations of Silurus asotus in different water systems in China based on mtDNA Cytb gene sequences[J].Journal of Fisheries of China,2017,41(10):1489-1499. |
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| Authors: | XU Dandan HUANG Yan ZENG Qing LI Bin and PENG Zuogang |
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| Affiliation: | Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, School of Life Sciences, Neijiang Normal University, Neijiang 641100, China,Key Laboratory of Freshwater Fish Reproduction and Development(Ministry of Education), Southwest University, Chongqing 400715, China,Key Laboratory of Freshwater Fish Reproduction and Development(Ministry of Education), Southwest University, Chongqing 400715, China,Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, School of Life Sciences, Neijiang Normal University, Neijiang 641100, China and Key Laboratory of Freshwater Fish Reproduction and Development(Ministry of Education), Southwest University, Chongqing 400715, China |
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| Abstract: | The catfish, Silurus asotus, is an important commercial fish in East Asia, and it is widely distributed throughout rivers and freshwater lakes of China.Unfortunately, population quantities of S.asotus decreased sharply in recent years because of overfishing and habitat fragmentation. Genetic diversity and population genetic structure are important aspects of the population genetics of fish, and provide essential information for understanding local adaptation and dispersal patterns, and for clarifying the genetic variation. Cytochrome b (Cytb) has a moderate evolutionary rate and a clear evolutionary pattern, suitable for the studies of phylogenetic evolution at the intra- and inter-specific levels. To identify the genetic diversity and population history among geographic populations of S.asotus in different water systems in China, including Yangtze River, Pearl River, Liao River and Southeast coastal rivers, and clarify its population demographic history and genetic structure, the genetic diversity, structure, differentiation, and molecular variance were analyzed using DNAsp5.10.01 and Arlequin 3.11. In the present study, 1110 bases of mtDNA Cytb gene were obtained from 216 of S. asotus collected in the different water systems in China from 2011 to 2012. Generally, there exists high diversity between haplotype (Hd=0.948±0.009) and nucleotide (Pi=0.017 99±0.000 55) in all populations of the catfish, suggesting that the populations were relatively large and stable. In addition, seventy-eight haplotypes, Hap45 was shared in individuals of all populations. Phylogenetic analysis of the MEGA5 was conducted to determine the relationships between S.asotus haplotypes, and detect discernible groups related to geography, and these analyses distinguished four inclusive clades. Moreover, the median-joining network was similar to the topology of the phylogenetic tree with 78 haplotypes, and revealed obivously four lineages in phylogeographic pattern. The results of neutrality tests on S.asotus data set of the four lineages, combined with the nuimodal mismatch distribution, indicated recent population expansion of S. asotus on large spatial scales in the period of late Pleistocene (0.04-0.05 Ma). |
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| Keywords: | Silurus asotus|cytochrome b (Cytb)|mtDNA|population history |
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