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盐度对点篮子鱼的存活、生长及抗氧化防御系统的影响
引用本文:王妤,庄平,章龙珍,刘鉴毅,赵峰.盐度对点篮子鱼的存活、生长及抗氧化防御系统的影响[J].水产学报,2011,35(1):66-73.
作者姓名:王妤  庄平  章龙珍  刘鉴毅  赵峰
作者单位:1. 大连海洋大学生命科学与技术学院,辽宁,大连,116023;中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源与生态重点开放实验室,上海,200090
2. 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源与生态重点开放实验室,上海,200090
3. 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源与生态重点开放实验室,上海,200090;中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种与养殖生物学重点开放实验室,江苏,无锡,214081
基金项目:上海市科技兴农重点攻关项目[沪农科攻字(2009)第5-7号];上海市科委科技成果转化项目(093919N1300);农业部淡水鱼类遗传育种和养殖生物学重点开放实验室课题(BZ2009-23);中央级公益性科研院所基本科研业务费专项资金项目(2008T02)
摘    要:在容积为500 L的圆锥形塑料缸中分别用对照(自然海水)、配制的盐度为20、10、5的海水和淡水(地下水)养殖点篮子鱼[(67.76?26.12) g],研究了不同盐度对点篮子鱼存活、生长和抗氧化酶活性的影响。结果显示,淡水组第9天出现死亡,至第27天时死亡率达100%,其余各组未出现异常,死亡率为0%。各盐度组鱼的特定生长率未表现出显著性差异,但盐度10组鱼体重显著高于盐度20和盐度5组,与对照组无显著性差异;盐度5组全长显著低于其余各盐度组。驯化40 d后,对各组鱼鳃、肝脏、肾脏和肌肉中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗超氧阴离子自由基、羟自由基活性检测结果显示,除盐度5组外,盐度10、20组点篮子鱼鳃、肝脏、肾脏、肌肉中SOD、CAT、抗超氧阴离子自由基和羟自由基活性在驯化40 d后均恢复到对照组水平,组间无显著性差异;盐度5组中鱼鳃的SOD和抗超氧阴离子自由基活性显著高于盐度10和盐度20组,肝脏、肌肉和肾脏中SOD、CAT、抗超氧阴离子自由基和羟自由基活性均恢复到对照组水平。点篮子鱼不同组织中SOD和CAT酶活力在不同盐度下均以肝脏中最高,肾脏和鳃次之,肌肉中最低;抗超氧阴离子自由基活力以肝脏中最高,极显著高于其余各组织中抗超氧阴离子自由基活力(P<0.01),肌肉次之,肾脏和鳃最低;羟自由基活力以肾脏中最高,显著高于其余各组织中羟自由基活力(P<0.05);鳃次之,肝脏和肌肉中最低,结果表明盐度能影响抗氧化酶活力大小,但并未影响鱼体内酶的分布。

关 键 词:点篮子鱼    盐度    抗氧化酶    自由基
收稿时间:2010/8/25 0:00:00
修稿时间:2010/11/9 0:00:00

Effects of salinity on survival,growth and antioxidant defense system of Siganus guttatus
WANG Yu,ZHUANG Ping,ZHANG Long-zhen,LIU Jian-yi and ZHAO Feng.Effects of salinity on survival,growth and antioxidant defense system of Siganus guttatus[J].Journal of Fisheries of China,2011,35(1):66-73.
Authors:WANG Yu  ZHUANG Ping  ZHANG Long-zhen  LIU Jian-yi and ZHAO Feng
Affiliation:WANG Yu1,2,ZHUANG Ping1,ZHANG Long-zhen1,LIU Jian-yi2,ZHAO Feng2,3(1.College of Aqua-life Science and Technology,Dalian Ocean University,Dalian 116023,China,2.Key and Open Laboratory of Marine and Estuarine Fisheries Resource and Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,3.Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Freshwater Fishery Research Center,Chi...
Abstract:Rabbit fish(Siganus guttatus)(67.76?26.12)g were reared under different salinities(fresh water,salinity 5,10,20 and control group(the local seawater))to study the response of survival,growth and antioxidant defense system to salinity.The rabbit fish which were fed twice everyday were held in 500 L conical plastic tank with 95 cm in height and 95 cm in diameter for 40 days and three parallels each group.After 40 d,six fish each group were taken randomly to collect the samples of the gill,liver,kidney and muscle.The results showed that the abnormal phenomenon was not found.The survival rate of salinity 5,10,20 and the control group during the experimental period was 100%.However,the abnormal phenomenon of the rabbit fish in fresh water was found(the food consumption was reduced)on the sixth day.They fasted from the 7th day and began to die from 9thd.Feed was recovered from the 13th day.However,death continued.The fish in fresh water died out after 27 d.Specific growth rate(SGR)in different salinities did not show significant differences.The body weight in salinity 10 was significantly higher than that in salinity 5 and 20.However,no significant difference was observed between salinity 10 and the control group.The total length in salinity 5 was significantly lower than other groups.In order to study the response of antioxidant defense system to salinity,the activity of superoxide dismutase(SOD),catalase(CAT),superoxide anion radical,hydroxyl radical of rabbit fish were measured.The activities of SOD,CAT,superoxide anion radical and hydroxyl radical in gill,liver,kidney,muscle of rabbit fish,reared under salinity 10,20,returned to normal levels(the level of control group)after 48 d.No significant differences were observed among each group of each index.However,the activities of SOD and superoxide anion radical in gill of rabbit fish under salinity 5 were significantly higher than that under salinity 10,20 and the control group.The activity of SOD in liver of rabbit fish was the highest among all groups,and followed by kidney and gill,muscle was the lowest and significantly lower than that in other tissues(P<0.05);the activity of CAT in liver of rabbit fish among all groups was the highest and significantly higher than that in other tissues(P<0.01),and followed by gill and kidney,muscle was the lowest;the activity of superoxide anion in liver of rabbit fish among all groups was the highest,and significantly higher than that in other tissues(P<0.01),and followed by muscle,kidney and gill were the lowest;the activity of hydroxyl radical in kidney of rabbit fish among all groups was the highest and significantly higher than that in other tissues(P<0.05);and followed by gill,liver and muscle were the lowest.The results showed that salinity can affect the activity of antioxidative enzymes,but did not affect the distribution.
Keywords:Siganus guttatus  salinity  antioxidant enzymes  free radical
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