| 缢蛏EGFR基因内含子1内SNP位点多态性与生长性状相关性 |
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| 引用本文： | 卫侃韵,谢淑媚,王沈同,陈宇宽,牛东红,李家乐.缢蛏EGFR基因内含子1内SNP位点多态性与生长性状相关性[J].水产学报,2019,43(2):483-491. |
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| 作者姓名： | 卫侃韵 谢淑媚 王沈同 陈宇宽 牛东红 李家乐 |
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| 作者单位： | 上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306;上海海洋大学, 水产动物遗传育种中心上海市协同创新中心, 上海 201306,上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306;上海海洋大学, 水产动物遗传育种中心上海市协同创新中心, 上海 201306;上海海洋大学, 上海水产养殖工程技术研究中心, 上海 201306 |
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| 基金项目： | 国家自然科学基金（31472278）；国家“八六三”高技术研究发展计划（2012AA10A400）；上海知识服务平台（ZF1206） |
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| 摘 要： | 为研究缢蛏表皮生长因子受体基因(epidermal growth factor receptor,EGFR)单核苷酸多态性(single nucleotide polymorphism, SNP)与生长性状(壳长、壳宽、壳高和体质量)的相关性。本实验利用直接测序法从缢蛏EGFR基因的第一个内含子序列中共筛选到17个SNP位点。卡方检验结果显示,在17个位点中,有13个位点符合Hardy-Weinberg平衡,位点多态性检测显示17个位点中有10个位点表现为中等多态性(0.25PIC0.5)。利用一般线性模型(general linear model,GLM)及多重比较对缢蛏EGFR基因中17个SNPs的多态性与生长性状(壳长、壳宽、壳高和体质量)进行相关性分析,结果显示,16个SNP位点均与缢蛏的壳长、壳宽、壳高及体质量呈显著性相关。由此可见,EGFR基因可作为缢蛏生长性状改良的候选辅助分子标记,并且为进一步研究其生长相关功能奠定基础。
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| 关 键 词： | 缢蛏 EGFR 多态性 生长性状 |
| 收稿时间： | 2017/11/16 0:00:00 |
| 修稿时间： | 2018/4/25 0:00:00 |
Polymorphism of SNPs in EGFR intron 1 and its association with growth traits in Sinonovacula constricta |
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| WEI Kanyun,XIE Shumei,WANG Shentong,CHEN Yukuan,NIU Donghong and LI Jiale.Polymorphism of SNPs in EGFR intron 1 and its association with growth traits in Sinonovacula constricta[J].Journal of Fisheries of China,2019,43(2):483-491. |
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| Authors: | WEI Kanyun XIE Shumei WANG Shentong CHEN Yukuan NIU Donghong and LI Jiale |
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| Affiliation: | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China and Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | In order to study the correlation between EGFR gene and growth traits (shell length, shell width, shell height and body weight), we analyzed the variation of EGFR intron 1 sequence from Sinonovacula constricta by using direct sequencing. There were 17 SNP sites in intron 1, named SNP1–SNP17 respectively. Based on Chi-square test, the 13 sites were fitted to Hardy-Weinberg equilibrium, and the 10 sites showed moderate polymorphism through polymorphism detection (0.25<PIC<0.5). The correlation between SNPs and growth traits (shell length, shell width, shell height, and body weight) were further analyzed using a General Linear Model and multiple comparisons, and the results showed that a total of 16 SNP sites were significantly associated with shell length, shell width, shell height and body weight. The results suggest that EGFR gene could be used as potential genetic marker for molecular breeding. The study also laid a foundation for further research on its growth function. |
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| Keywords: | Sinonovacula constricta EGFR polymorphism growth traits |
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