| 球等鞭金藻生长抑制物对自身藻细胞的抑制效应 |
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| 引用本文： | 阎斌伦,孙颖颖,王长海.球等鞭金藻生长抑制物对自身藻细胞的抑制效应[J].水产学报,2009,33(3):456-461. |
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| 作者姓名： | 阎斌伦 孙颖颖 王长海 |
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| 作者单位： | 1. 淮海工学院江苏省海洋生物技术重点实验室,江苏,连云港,222005
2. 烟台大学海洋学院,山东,烟台,264005;大连理工大学环境科学与生命学院,辽宁,大连,116024 |
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| 基金项目： | 国家基础研究重大项目基金 |
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| 摘 要： | 研究了生长抑制物1-羟基，丙二酸二乙酯-十二烯酸异丙酯（HDMA）对自身细胞生长、叶绿素和丙二醛（MDA）含量、胞外可溶性蛋白质和多糖含量、硝酸还原酶（NR）、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性等生理指标的影响，分析HDMA对球等鞭金藻的抑制效应。结果表明，HDMA显著降低了细胞密度、叶绿素含量以及NR、SOD和POD活性，显著增大胞外可溶性蛋白质和多糖的比值，并且还明显提高MDA含量。因此，我们认为HDMA能够影响微藻细胞的某些生理生化过程。其中包括降低叶绿素含量，影响光合作用；改变胞外可溶性蛋白质和多糖的比值，增强细胞的疏水性，促使细胞絮凝；降低微藻硝酸还原酶和抗氧化体系酶的活性，影响细胞内营养平衡，并加速细胞体内活性氧的积累，导致细胞发生过氧化反应，从而影响微藻生长。
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| 关 键 词： | 球等鞭金藻 生长抑制物 抑制效应 分析 |
| 收稿时间： | 5/30/2008 5:57:13 PM |
| 修稿时间： | 2009/1/16 0:00:00 |
Inhibitory effects of growth-inhibitor of Isochrysis galbana on itself cells |
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| YAN Binlun,SUN Yingying and WANG Changhai.Inhibitory effects of growth-inhibitor of Isochrysis galbana on itself cells[J].Journal of Fisheries of China,2009,33(3):456-461. |
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| Authors: | YAN Binlun SUN Yingying and WANG Changhai |
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| Affiliation: | Huaihai Institute of Technology,Huaihai Institute of Technology,Yantai University /Dalian University of Technology |
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| Abstract: | 1-hydroxyl-diethyl malonate]-isopropyl dodecenoic acid (C22H38O7), a type of growth-inhibitor, was successful isolated and purified from cultural liquid of Isochrysis galbana. A study was planned to investigate the inhibitory effects of HDMA on the growth of Isochrysis galbana. Results showed that exogenously added HDMA inhibited the growth of this algae. HDMA significantly decreased the chlorophyll contents of algal cells. And it had a very significant effect on the ration of extracellular protein to polysaccharide and activities of NR (nitrate reductase), SOD (superoxide dismutase) and POD (peroxidase) of algal cells. But it was obvious to increase MDA (malonyldialdehyde) content of algal cells. The results gave hints to elucidate the species-specific antialgal mechanisms of HDMA. Inhibition mechanism of HDMA on Isochrysis galbana is to decrease the chlorophyll contents, and decrease or increase activities of NR, SOD and POD of algal cells, and change the ratio of extracellular protein to polysaccharide, thus increase in ratio of extracellular protein to polysaccharide leads to the increase in hydrophobicity of algal cells, finally algal cells flocculate and subside. Affect uptake of inorganic nitrogen by cells and photosynthesis might play a major in the inhibitory mechanism. And the excess active oxygens were produced under HDMA stress and active oxygen participated in the damage of HDMA to this algae. It indicated that the physiological metabolism of Isochrysis galbana was inhibited by HDMA. |
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| Keywords: | Isochrysis galbana growth-inhibitor inhibitory effect analysis |
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