| 玉米蛋白粉替代鱼粉对暗纹东方鲀溶菌酶活性及c型溶菌酶mRNA表达的影响 |
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| 引用本文： | 钟国防,钱曦,华雪铭,周洪琪.玉米蛋白粉替代鱼粉对暗纹东方鲀溶菌酶活性及c型溶菌酶mRNA表达的影响[J].水产学报,2010,34(7):1121-1128. |
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| 作者姓名： | 钟国防 钱曦 华雪铭 周洪琪 |
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| 作者单位： | 上海海洋大学省部共建水产种质资源发掘与利用教育部重点开放实验室,上海,201306 |
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| 基金项目： | 上海市科委重大科技攻关项目(06DZ19102,073205111); 农业部水产种质资源与利用重点开放实验室开放课题(KFT2008-2) |
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| 摘 要： | 以初重为(41.26±1.09)g的暗纹东方鲀为实验鱼,配制5种玉米蛋白粉使用量为0%、5%、10%、15%、20%的实验饲料,玉米蛋白粉对饲料中鱼粉的替代水平分别为0%、7.4%、14.8%、22.2%和29.6%。用实验饲料饲养暗纹东方鲀60d,研究玉米蛋白粉替代鱼粉对暗纹东方鲀肝胰脏、头肾和脾脏组织的溶菌酶活性及其c-型溶菌酶mRNA相对表达量的影响。结果表明,(1)暗纹东方鲀肝胰脏、头肾和脾脏的溶菌酶比活力分别为9.14、42.12和40.49U/mgprot,头肾和脾脏组织中c-型溶菌酶mRNA相对表达量分别是肝胰脏的3.76和3.24倍。(2)玉米蛋白粉替代鱼粉显著影响暗纹东方鲀溶菌酶比活力。5%和10%组的肝胰脏和头肾组织溶菌酶比活力显著高于对照组(P<0.05),而15%和20%组肝胰脏和头肾组织溶菌酶比活力则显著低于对照组(P<0.05);5%和10%组的脾脏组织溶菌酶活力与对照组无显著性差异(P>0.05),而15%和20%组显著低于对照组和5%组(P<0.05)。(3)玉米蛋白粉对暗纹东方鲀c型溶菌酶基因mRNA相对表达量有显著影响(P<0.05)。肝胰脏组织中5%和10%组表达量显著高...
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| 关 键 词： | 暗纹东方鲀 玉米蛋白粉 溶菌酶活性 溶菌酶基因mRNA表达 |
| 收稿时间： | 2009/11/16 0:00:00 |
| 修稿时间： | 2010/4/22 0:00:00 |
Effects of feeding with corn gluten meal on lysozyme activity and c-lysozyme gene expression in Fugu obscurus |
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| Qian xi,Hua Xueming and Zhou Hongqi.Effects of feeding with corn gluten meal on lysozyme activity and c-lysozyme gene expression in Fugu obscurus[J].Journal of Fisheries of China,2010,34(7):1121-1128. |
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| Authors: | Qian xi Hua Xueming and Zhou Hongqi |
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| Affiliation: | ZHONG Guo-fang,QIAN Xi,HUA Xue-ming,ZHOU Hong-qi(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Shanghai 201306,China) |
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| Abstract: | To examine the effects of corn gluten meal on lysozyme activity and c-lysozyme gene expression in Fugu obscurus,the fish were fed with diets containing 0%,5%,10%,15% and 20% corn gluten meal in replacement of 0%,7.4%,14.8%,22.2% and 29.6% of conventional fish meal for 60 days,respectively.Lysozyme activities and mRNA levels in the hepatopancreas,head kidney and spleen were examined.The results show that:(1) At the beginning of the experiment,the lysozyme activities of the hepatopancreas,head kidney and sple... |
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| Keywords: | Fugu obscurus corn gluten meal lysozyme activity lysozyme gene expression |
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