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| 微卫星标记分析乌龟养殖群体的遗传多样性 | |
| 引用本文： | 章芸,俞丹娜,杜卫国,郑荣泉,杨光.微卫星标记分析乌龟养殖群体的遗传多样性[J].水产学报,2010,34(11):1636-1644. |
| 作者姓名： | 章芸 俞丹娜 杜卫国 郑荣泉 杨光 |
| 作者单位： | 1. 浙江师范大学生态研究所,浙江,金华,321004 2. 杭州师范学院生命科学学院,浙江,杭州,310036 3. 南京师范大学生命科学学院,江苏,南京,210046 |
| 基金项目： | 国家自然科学基金项目(30970362) |
| 摘 要： | 利用8个微卫星标记对7个乌龟养殖群体进行了遗传多样性和遗传结构的检测。结果显示,7个养殖群体都表现出较高的多态性,8个位点共检测出130个等位基因,范围在9～26,平均16.25;其多态信息含量(PIC)范围为0.57～0.92,平均值为0.71;观测杂合度(HO)和期望杂合度(HE)分别为0.30～0.87和0.60～0.93。分子变异方差分析(AMOVA)结果表明,遗传变异5.91%来自群体间,84.29%来自群体内部,两两群体间FST值在0.0143～0.1127,其中57.14%的两两群体间无分化,42.86%的两两群体间出现了中等程度的分化。哈迪-温伯格平衡检测表明,8个位点中有5个位点显著或极显著的偏离了哈迪-温伯格平衡,推测各群体中出现了近交繁殖的现象。7群体间遗传距离为0.1066～0.6468,UPGMA聚类分析表明,湖北荆州群体单独聚为一支,其余6个群体聚为另一支。另外,7个群体都存在特有等位基因,提示群体间等位基因扩散受到一定程度的限制,同时在育种上,可以作为亲本选育的一个重要参考指标。 |
| 关 键 词： | 乌龟 遗传多样性 微卫星 |
| 收稿时间： | 2010/5/15 0:00:00 |
| 修稿时间： | 9/6/2010 12:00:00 AM |
Microsatellite DNA analysis of genetic diversity among captive breeding stocks of Chinese pond turtle(Chinemys reevesii) |
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| ZHANG Yun,YU Da-n,DU Wei-guo,ZHENG Rongquan and YANG Guang.Microsatellite DNA analysis of genetic diversity among captive breeding stocks of Chinese pond turtle(Chinemys reevesii)[J].Journal of Fisheries of China,2010,34(11):1636-1644. | |
| Authors: | ZHANG Yun YU Da-n DU Wei-guo ZHENG Rongquan and YANG Guang |
| Affiliation: | ZHANG Yun1,YU Dan-na1,DU Wei-guo2,ZHENG Rong-quan1,YANG Guang3 (1.Institute of Ecology,Zhejiang Normal University,Jinhua 321004,China,2.College of Biology,Hangzhou Normal University,Hangzhou 310036,3.Jiangsu Key Laboratory for Biodiversity and Biotechnology,College of Life Sciences,Nanjing Normal University,Nanjing 210046,China) |
| Abstract: | Chinese pond turtle(Chinemys reevesii Gray,1831) is one of the main turtle species in China with commercial and medicinal importance,and it began to attract attention as a breeding species in 1990s and became one of the largest domestic breeding varieties.The population of farmed Chinese pond turtle increased in China,however,only little is known on the genetic status of this species.In this study,eight highly variable microsatellite loci were used to investigate genetic diversity and captive stocks structu... |
| Keywords: | Chinese pond turtle (Chinemys reevesii) genetic diversity microsatellite |
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