| 基于线粒体Cyt b分析入侵云南四大水系的麦穗鱼群体遗传结构 |
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| 引用本文： | 杨丽萍,胡俊仪,秦超彬,张玉茹,卢荣华,孟晓林,杨国坤,闫潇,职韶阳,聂国兴.基于线粒体Cyt b分析入侵云南四大水系的麦穗鱼群体遗传结构[J].水产学报,2020,44(3):339-350. |
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| 作者姓名： | 杨丽萍 胡俊仪 秦超彬 张玉茹 卢荣华 孟晓林 杨国坤 闫潇 职韶阳 聂国兴 |
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| 作者单位： | 河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007;河南师范大学水产学院,河南新乡 453007 |
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| 基金项目： | 国家自然科学基金（U1504304）；河南省重点科技攻关项目（182102110284）；河南省创新型科技团队（CXTD2016043） |
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| 摘 要： | 为揭示麦穗鱼入侵云南后群体的遗传多样性和遗传分化差异现状,实验采集了云南澜沧江、怒江、红河、伊洛瓦底江水系13个样点,及黄河、长江、珠江原产地水系6个样点的麦穗鱼群体共计220尾样本,利用线粒体细胞色素b基因(Cyt b)全序列作为分子标记,初步分析了麦穗鱼群体的遗传多样性、遗传结构和遗传分化情况。结果显示,共检测到72个变异位点,定义25个Cyt b单倍型。云南四大水系麦穗鱼单倍型多样性和核苷酸多样性分别为0.828±0.014和0.005 44±0.001 18。云南四大水系和黄河、长江、珠江水系相比,具有较高的遗传多样性。单倍型系统发育树与单倍型网络图显示,黄河群体单倍型独立,云南各水系单倍型与珠江、长江单倍型混杂,推测云南麦穗鱼主要来源于珠江和长江,这与云南省引种经济鱼类历史一致。分子变异分析(AMOVA)显示,云南四大水系麦穗鱼群体间具有程度较高的遗传分化,其中大多数遗传变异存在于群体内(72.60%),群体间的遗传变异为28.62%,水系间为1.22%。结果发现麦穗鱼遗传分化与当前水系的分布格局不吻合。Fu’s Fs中性检验结果和核苷酸不配对分析结果均表明,云南四大水系麦穗鱼群体未发生扩张。麦穗鱼进入云南各水系后,单倍型多样性较高,可能来源于多个地区。在后续对麦穗鱼的管理过程中,需要注意避免单倍型特殊的群体与其他地区群体的交流,减少水系间相互引种。此外,通过开发麦穗鱼资源利用方式来提高麦穗鱼利用率,以控制其群体数量,从而减小其对当地土著物种和渔业养殖的危害。
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| 关 键 词： | 麦穗鱼 遗传多样性 遗传结构 细胞色素b基因 |
| 收稿时间： | 2019/2/20 0:00:00 |
| 修稿时间： | 2019/6/22 0:00:00 |
Genetic structure analysis of Pseudorasbora parva in the four major river systems in Yunnan based on mitochondrial Cyt b |
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| YANG Liping,HU Junyi,QIN Chaobin,ZHANG Yuru,LU Ronghu,MENG Xiaolin,YANG Guokun,YAN Xiao,ZHI Shaoyang and NIE Guoxing.Genetic structure analysis of Pseudorasbora parva in the four major river systems in Yunnan based on mitochondrial Cyt b[J].Journal of Fisheries of China,2020,44(3):339-350. |
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| Authors: | YANG Liping HU Junyi QIN Chaobin ZHANG Yuru LU Ronghu MENG Xiaolin YANG Guokun YAN Xiao ZHI Shaoyang and NIE Guoxing |
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| Affiliation: | College of Fisheries, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China and College of Fisheries, Henan Normal University, Xinxiang 453007, China |
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| Abstract: | Pseudorasbora parva was originally distributed in Jinshajiang and Nanpanjiang in Yunnan. However, it has been widely distributed in all major river systems and has become an invasive fish in other four river systems in Yunnan. In order to reveal the difference of genetic diversity and genetic differentiation of P. parva between Yunnan after the invasion and the native region, we collected 220 samples distributed in 13 different sites of Lancang River, Nujiang, Honghe and Irrawaddy River in Yunnan, and 6 native populations in Yellow River, Yangtze River, and Pearl River. Here we analysed a 1 072 base pair fragment of the mitochondrial cytochrome b gene (Cyt b) to examine the genetic diversity and genetic structure. A total of 72 mutation sites and 25 Cyt b haplotypes were identified. The haplotype diversity and nucleotide diversity of the four river systems in Yunnan were 0.828±0.014 and 0.005 44±0.001 18, respectively. Haplotype diversity of the introduced populations from the four river systems in Yunnan was higher than that of the native populations. The haplotype maximum likelihood tree and haplotype network map show that the haplotypes of the Yellow River population are special, while haplotypes in the invasive populations in Yunnan were mixed with those in Yangtze River and Pearl River. It is speculated that P. parva in Yunnan mainly came from the Yangtze River and the Pearl River, which was consistent with the history of introduction of economic fish in Yunnan Province. Analysis of molecular variance (AMOVA) revealed significant genetic subdivision among individuals within populations and among populations within rivers but not among rivers, indicating distribution of genetic diversity was inconsistent with contemporary hydrological structure. That may be the human effect who introduced them from many native regions. The results of Fu''s Fs neutral test showed that there was no expansion of the invasive population of P. parva in Yunnan. In the subsequent management of the invasive population of P. parva in Yunnan, it is necessary to avoid the exchange of special haplotype groups with other regional groups, and to reduce the introduction of fish from different river systems. In addition, the development of new ways to utilize P. parva may be a useful way to control their population and reduce the damage to indigenous species and fishery culture in Yunnan. |
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| Keywords: | Pseudorasbora parva genetic diversity genetic structure Cyt b |
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