| 白斑症病毒在日本对虾体内的感染增殖 |
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| 引用本文： | 战文斌.白斑症病毒在日本对虾体内的感染增殖[J].水产学报,1999,23(3):278-282. |
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| 作者姓名： | 战文斌 |
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| 作者单位： | [1]教育部水产养殖开放实验室 [2]东京水产大学 |
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| 基金项目： | 国家863 资助项目，教育部留学回国人员科研启动基金 |
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| 摘 要： | 用投喂患白斑症病毒病的虾组织人工感染日本对虾稚虾，每日取样，整虾冰冻切片，单克隆抗体的荧光抗体方法，原位观察病毒在虾体内的感染增殖，结果表明：感染后三天内，在感染虾的各组织器官内均未观察到明显的病毒感染的阳性细胞，每四天首先在鳃丝腔内的小量血细胞观察到病毒感染；第五天除血细胞外同时在血窦，鳃上皮组织，皮下组织内观察到，第六天进而在心脏，胃上皮组织内观察到：第七天进一步又在淋巴器官，中肠内观察到，八
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| 关 键 词： | 日本对虾 白斑症病毒 感染增殖 |
| 收稿时间： | 2014/4/18 0:00:00 |
| 修稿时间： | 2014/4/18 0:00:00 |
INFECTION DEVELOPMENT OF WHITE SPOT SYNDROME VIRUS IN THE BODY OF PENAEUS JAPONICUS |
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| ZHAN Wen Bin.INFECTION DEVELOPMENT OF WHITE SPOT SYNDROME VIRUS IN THE BODY OF PENAEUS JAPONICUS[J].Journal of Fisheries of China,1999,23(3):278-282. |
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| Authors: | ZHAN Wen Bin |
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| Affiliation: | ZHAN Wen Bin,WANG Yuan Hong,SUZUKI Shinichi *,YU Kai Kang,FUKUDA Hideo * |
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| Abstract: | Juvenile Penaeus japonicus were experimentally infected by feeding minced shrimp tissue of WSSV infected. The experimental shrimps were sampled everyday and whole body of the shrimp was cryosectioned then WSSV was detected by FAT method using monoclonal antibodies in order to explain the virus development in shrimp body. The results indicated that in the first three days post infection the virus was not detected in any samples; on the forth day the virus was first detected in hemacyte in gill cavity; on the fifth day it developed to gill tissue, epidermal tissue and hemal sinus; on the sixth day to stomach and heart; on the seventh day to lymphoid organ and mid gut; on the eighth and ninth days to all the detected tissues but not to the hepatopancreas. |
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| Keywords: | Penaeus japonicus White spot syndrome virus(WSSV) Infection development |
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