| 青岛地区常见鱼类过敏原的鉴定及其交叉反应研究 |
| |
| 引用本文： | 吴玟静,李振兴,金科,郑礼娜,王邦平,林洪.青岛地区常见鱼类过敏原的鉴定及其交叉反应研究[J].水产学报,2011,35(10):1532-1539. |
| |
| 作者姓名： | 吴玟静 李振兴 金科 郑礼娜 王邦平 林洪 |
| |
| 作者单位： | 中国海洋大学食品安全实验室,山东青岛,266003 |
| |
| 基金项目： | 国家自然科学基金项目(30800859);现代农业产业技术体系建设专项资金(CARS-50) |
| |
| 摘 要： | 选择青岛地区喜食的阿拉斯加狭鳕、蓝点马鲛、大黄鱼、真鲷、鲑、大菱鲆、鲤 等7种鱼为研究对象,利用鱼类过敏患者血清,通过SDS-PAGE及免疫印迹方法对每种鱼的过敏原蛋白进行鉴定。利用兔抗鲅鱼小清蛋白多克隆抗体,通过间接ELISA和抑制性ELISA等对7种鱼的小清蛋白之间的交叉反应进行探讨。结果表明,青岛地区居民鱼类过敏原主要为48~57、33~41、28 及17 ku的蛋白,和传统公认的小清蛋白为主要过敏原的共识不同。而针对小清蛋白的交叉反应研究也表明,不同鱼类蛋白之间存在强烈的交叉反应。因此,虽然鱼类过敏原可能不同,但如果对以上7种鱼中的一种鱼过敏,最好不要食用其他任何一种鱼。
|
| 关 键 词： | 鱼 过敏原 鉴定 交叉反应 |
| 收稿时间： | 4/15/2011 4:13:41 PM |
| 修稿时间： | 7/6/2011 10:04:13 AM |
Study on the identification of allergens from 7 fishes commonly consumed in Qingdao and their cross-reactivity |
| |
| WU Wen-jing,LI Zhen-xing,JIN Ke,ZHENG Li-n,WANG Bang-ping and LIN Hong.Study on the identification of allergens from 7 fishes commonly consumed in Qingdao and their cross-reactivity[J].Journal of Fisheries of China,2011,35(10):1532-1539. |
| |
| Authors: | WU Wen-jing LI Zhen-xing JIN Ke ZHENG Li-n WANG Bang-ping and LIN Hong |
| |
| Affiliation: | Food Safety Laboratory, Ocean University of China,Food Safety Laboratory, Ocean University of China,Food Safety Laboratory, Ocean University of China,Food Safety Laboratory, Ocean University of China,Food Safety Laboratory, Ocean University of China,College of food science and engineering,Ocean university of China |
| |
| Abstract: | Qingdao is a coastal city of China where the problem of fish allergy is increasingly prominent.There have been no data providing a comprehensive picture of the major fish allergens to local people so far.Based on the above situation,the aim of this research was to study on the cross-reactivity of different fish allergens,to provide primary basis of safe eating guide for local fish allergic individuals.To determine the allergens of 7 commonly edible fishes:Alaska pollock(Theragra chatcogramma),red snapper(Pagrosomus major),salmon(Salmo salar),spanish mackerel(Scomberomrus niphonius),turbot(Scophthatmus maximus),large yellow croaker(Larimichthys crocea) and carp(Cyprinus carpio),SDS-PAGE was applied to observe the protein components of fish extracts.Sera from 18 fish allergic patients were used in Western-blot.Cross-reactivity was investigated by IgG Western-blot,IgG ELISA and ELISA inhibition used rabbit anti-spanish mackerel parvalbumin polyclonal antibody.Results showed that the major fish allergens were 48-57,33-41 ku,28 ku and 17 ku proteins to Qingdao residents,which was distinct from the common consensus that parvalbumin was the major allergen in fish.Meanwhile,the study on the cross-reactivity of fish parvalbumin also indicated there was a quite strong cross-reactivity between different species of fish proteins.Therefore,despite the allergens might be different from each other,it was suggested that the subjects not eat other 6 fishes if allergic to one of them. |
| |
| Keywords: | fish allergen identification cross-reactivity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
|
点击此处可从《水产学报》下载全文 |
|
