| 海芦笋黄酮的抗氧化作用及对CCl4致小鼠急性肝损伤的保护作用 |
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| 引用本文： | 段筱杉,张朝辉,应锐,赵腾飞,刘奥,李八方,赵雪,侯虎.海芦笋黄酮的抗氧化作用及对CCl4致小鼠急性肝损伤的保护作用[J].水产学报,2017,41(12):1946-1955. |
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| 作者姓名： | 段筱杉 张朝辉 应锐 赵腾飞 刘奥 李八方 赵雪 侯虎 |
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| 作者单位： | 中国海洋大学食品科学与工程学院,山东 青岛,266003 |
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| 基金项目： | 国家自然科学基金（31272705） |
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| 摘 要： | 为深入探究海芦笋黄酮的活性作用,研究海芦笋黄酮在体外的抗氧化作用及其对CCl_4导致的小鼠急性肝损伤的预防保护作用。通过分析海芦笋黄酮对4种自由基(DPPH自由基、ABTS自由基、羟基自由基及超氧阴离子自由基)的清除能力反映海芦笋黄酮的体外抗氧化能力。将海芦笋黄酮分为低、中、高3个剂量75、150、300 mg/(kg·d)]对小鼠连续灌胃8 d,通过腹腔注射CCl_4建立小鼠急性肝损伤模型,测定海芦笋黄酮对血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)的活性以及肝脏组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽(GSH)水平的影响,观察肝脏病理变化。结果显示,在体外抗氧化实验中,海芦笋黄酮对4种自由基都有良好的清除效果。在CCl_4急性肝损伤模型中,与模型组对比,各剂量海芦笋黄酮均能使血清中ALT、AST、ALP酶活性、肝脏MDA含量显著降低;使肝脏SOD活性、GSH含量显著增加。海芦笋黄酮中、高剂量组与模型组相比,肝脏CAT活性显著升高。肝脏切片结果显示各剂量组肝组织损伤情况有不同程度改善。海芦笋黄酮对由CCl_4引起的小鼠急性肝损伤具有一定程度的预防保护作用,其急性肝损伤保护机制可能与黄酮对脂质过氧化程度的削弱、机体抗氧化能力的提高有关。
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| 关 键 词： | 海芦笋 黄酮 抗氧化 四氯化碳 急性肝损伤 |
| 收稿时间： | 2016/11/15 0:00:00 |
| 修稿时间： | 2017/3/6 0:00:00 |
Antioxidant and protective effect of flavonoids from Salicornia bigelovii against CCl4-induced acute hepatic injury in mice |
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| DUAN Xiaoshan,ZHANG Zhaohui,YING Rui,ZHAO Tengfei,LIU Ao,LI Bafang,ZHAO Xue and HOU Hu.Antioxidant and protective effect of flavonoids from Salicornia bigelovii against CCl4-induced acute hepatic injury in mice[J].Journal of Fisheries of China,2017,41(12):1946-1955. |
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| Authors: | DUAN Xiaoshan ZHANG Zhaohui YING Rui ZHAO Tengfei LIU Ao LI Bafang ZHAO Xue and HOU Hu |
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| Affiliation: | College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China,College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China and College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China |
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| Abstract: | To investigate the antioxidant effects and the protective effects of the flavonoids from Salicornia bigelovii on CCl4-induced acute hepatic injury in mice, the scavenging DPPH, ABTS, hydroxyl free radicals and superoxide anion free radicals abilities of the flavonoids from S. bigelovii were studied to show its antioxidant ability in vitro. Mice with acute hepatic injury were simulated by intraperitoneal injecting CCl4. Before simulation, mice were administered with the flavonoids everyday at doses of 75, 150, 300 mg/(kg·d) for 8 days. After that, mice in each group were sacrificed to determine the indexes including the enzyme activity of ALT, AST, ALP in the serum and MDA, GSH, CAT, SOD in the hepatic tissue. Meanwhile, the pathological changes in the hepatic tissues of each group were determined. In the experimental concentration range, the flavonoids from S. bigelovii have a good effect on scavenging four free radicals. Compared with the simulation group, the level of serum ALT, AST, ALP and liver MDA in each dose decreased significantly, and levels of SOD, GSH rose. The level of liver CAT in high and middle dose group was higher than that in the simulation group significantly. The result of the tissue section after H.E staining showed that the damage of liver tissues in each dose had different degrees of improvement. S. bigelovii flavonois can obviously protect against CCl4-induced acute liver injury in mice, and the protective mechanism is related to the fact that flavonoids can enhance antioxidant capacity and relieve membrane lipid per-oxidation. |
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| Keywords: | Salicornia bigelovii flavonoids antioxidant CCl4 acute hepatic injury |
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