| 腹腔注射葡萄糖对大黄鱼血液生化指标及糖代谢关键酶表达量的影响 |
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| 引用本文： | 李弋,邱红,侯迎梅,陆游,申屠基康,周歧存.腹腔注射葡萄糖对大黄鱼血液生化指标及糖代谢关键酶表达量的影响[J].水产学报,2017,41(5):723-733. |
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| 作者姓名： | 李弋 邱红 侯迎梅 陆游 申屠基康 周歧存 |
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| 作者单位： | 1. 宁波大学海洋学院,鱼类营养研究室,浙江宁波 315211;2. 宁波市海洋与渔业研究院,浙江宁波,315211 |
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| 基金项目： | (31272670)；宁波市农业科技攻关重大项目(2012C10025)；浙江省2011协同创新项目；浙江省重中之重一级学科建设项目；岱衢洋大黄鱼专用饲料集成应用与示范(2014GA701001) |
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| 摘 要： | 为研究大黄鱼对葡萄糖的耐受能力和相关糖代谢关键酶的表达量,选取体质量约为100 g的大黄鱼禁食24 h,随机分为3个实验组,分别为对照组(0.9%的无菌生理盐水,CG组),低剂量葡萄糖组(300 mg/kg体质量,LG组)和高剂量葡萄糖组(1500 mg/kg体质量,HG组)。结果显示,大黄鱼在注射高剂量和低剂量葡萄糖后,血糖均在3 h达到最高水平,且HG组的峰值显著高于LG组,LG组在24 h恢复至正常水平,HG组在24 h仍然高于正常水平。HG组、LG组注射葡萄糖后,HK、GK基因相对表达量均显著上升,其峰值出现在注射后9 h。HG组PFK基因相对表达量在注射后6 h达到峰值。HG组PEPCK基因相对表达量在注射葡萄糖后显著下降,其中最低时间点为2 h。HG组G6PD基因相对表达量在注射后6 h显著高于其他各个时间点。研究表明,注射高、低浓度葡萄糖后,均会提高大黄鱼血糖水平,且能维持较长时间。注射葡萄糖后糖酵解途径关键酶如HK、GK、PFK及糖异生途径关键酶PEPCK基因相对表达量受血糖调节,但G6Pase、FBPase表达量并不因血糖升高而下降。导致注射葡萄糖后,大黄鱼不断产生内源性葡萄糖,这是大黄鱼表现为对高血糖不耐受的原因之一。
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| 关 键 词： | 大黄鱼 腹腔注射 糖耐量 糖代谢关键酶 基因表达 |
| 收稿时间： | 2016/6/3 0:00:00 |
| 修稿时间： | 2016/10/9 0:00:00 |
Effects of glucose injection on serum indices and gene expression of carbohydrate metabolic enzymes in large yellow croaker (Larimichthys crocea) |
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| LI Yi,QIU Hong,HOU Yingmei,LU You,SHENTU Jikang and ZHOU Qicun.Effects of glucose injection on serum indices and gene expression of carbohydrate metabolic enzymes in large yellow croaker (Larimichthys crocea)[J].Journal of Fisheries of China,2017,41(5):723-733. |
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| Authors: | LI Yi QIU Hong HOU Yingmei LU You SHENTU Jikang and ZHOU Qicun |
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| Affiliation: | Laboratory of Fish Nutrition,School of Marine Sciences,Laboratory of Fish Nutrition,School of Marine Sciences,Laboratory of Fish Nutrition,School of Marine Sciences,Laboratory of Fish Nutrition,School of Marine Sciences,Ningbo Ocean and Fishery Research Institute,Laboratory of Fish Nutrition,School of Marine Sciences |
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| Abstract: | This experiment was conducted to evaluate the effects of intraperitoneal glucose injection on serum indices, hepatic glycolysis and gluconeogenic key enzyme expression levels of intraperitoneal glucose injection of large yellow croaker (Larimichthys crocea). Fish n=270, mean weight=(100±5) g] were randomly assigned to three groups, a control group that was injected with sterile 0.9% normal saline, the low dosage group that was injected with glucose (300 mg/kg wet weight, LG) and the high dosage group with glucose (1500 mg/kg wet weight, HG). The results showed that the plasma glucose level reached a peak at 3 h after both dose glucose injections, and returned to base level at the time of 24 h after injection of low dosage glucose, and it was still higher than base level after 24 h after high dosage glucose relatively. The trend of relative expression of HK gene after both dose glucose injections was increased first, then decreased, and reached the peak at 9 h. The relative expression of GK gene trend was to rise first and then remained unchanged and finally fell after both dose glucose injections, and it reached the peak at 9 h, but it was decreased at 4 h after high dosage glucose. The relative expression of PFK gene presented the trend of first increasing and then decreasing, and it reached the highest at 9 h after high dosage glucose. The trend of relative expression of PEPCK gene decreased first and then increased, and it reached the minimum at 2 h after high dosage glucose. It was significantly higher at 6 h than at any other time point, after high dosage glucose. These results indicate that the plasma glucose level of large yellow croaker increased, and maintained for a long time. The absence of inhibition in the expression of the gluconeogenic enzyme of G6Pase,FBPase concurrent with increased postprandia expression of GK led to postprandial hyperglycaemia in large yellow croaker. |
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| Keywords: | Larimichthys crocea glucose tolerance carbohydrate metabolic enzymes gene expression |
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