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In Vitro and In Vivo Feedstuff Digestibility for Snook,Centropomus undecimalis,Juveniles
Authors:Iratzio Lemus  Carlos Maldonado  Gerard Cuzon  Adolfo Sanchez  Gabriela Gaxiola  Alfonso Alvarez  Martha Guerrero
Affiliation:1. Posgrado de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de Mexico, Avenida Universidad 3000, Col. UNAM‐CU, Coyoacan, Mexico;2. Unidad Multidisciplinaria de Docencia e Investigación de Sisal, Facultad Ciencias, Universidad Nacional Autónoma de Mexico, Puerto de Abrigo S/N Hununcmá, Yucatán cp 97355, México;3. Laboratorio de Acuicultura Tropical, DACBIOL. Universidad Juárez Autónoma de Tabasco. Carrtera Villaermosa‐Cárdenas km 0.5. Villahemrosa, Tabasco, México;4. Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Ave Pedro de Alba S/N;5. San Nicolás de los Garza, Nuevo Léon, México
Abstract:The degree of hydrolysis (DH) of the ingredients was evaluated for Centropomus undecimalis juveniles. The in vitro experiment included wheat gluten (WG), corn gluten (CG), soybean meal (SBM) and pretreated phytase (SBM + phytase), soy protein concentrate (SPC), canola meal (CAN) and pretreated phytase (CAN + phytase), poultry byproduct meal (PBPM), chicken meal (CHKM), dried whey (DW), Pota meal (PM: mix of giant squid, fish soluble protein concentrate, crustacean meal, and fish oil), and Protiblend (PTB: aquatic and render mix). The highest acidic DH occurred with PTB (0.38 ± 0.06), CHKM (0.33 ± 0.3), and PBPM (0.25 ± 0.03). In the alkaline condition, PTB (1.6 ± 0.17 and 0.98 ± 0.05 for pyloric caeca and intestine, respectively) and CG (1.04 ± 0.4 and 0.75 ± 0.2 for caeca and intestine, respectively) provided the highest DH values. In vitro digestibility demonstrated that PTB was the highest (85.3%) followed by PBPM (51.4%), CAN + phytase (47.6%), CG (45.1%), and CHKM (46.5%). The in vivo experiment concerned the WG, CHKM, PBPM, PM, and PTB diets plus a reference diet with fishmeal (Ref diet). The total %DH was different (P < 0.05) with the lowest values for the WG diet (0.34 ± 0.09%) and Ref diet (0.34 ± 0.15). Free amino acid released during digestion was displayed for these diets and a bifactorial analysis produced no difference (P > 0.05). The apparent digestive coefficients ranged from 89.8 to 92.9% for protein and from 68 to 71.4% for energy.
Keywords:amino acids  in vitro and in vivo digestibility  pH‐stat  protein  snook
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