RNA/DNA ratio is an early responding,accurate performance parameter in growth experiments of noble crayfish Astacus astacus (L.) |
| |
| Authors: | Claudia Grimm Kai Lehmann Catriona Clemmesen Heinz Brendelberger |
| |
| Affiliation: | 1. Department of Limnology, Zoological Institute at Kiel University, Kiel, Germany;2. GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany |
| |
| Abstract: | Recent intensification efforts of astacid culture considerably depend on the appropriate assessment of the animal's physiological condition both for research and application. We conducted a 4 weeks feeding experiment to assess temporal resolution and accuracy of different response parameters (RNA/DNA ratio, RNA per wet weight, carapace length, wet weight, specific growth rate). Juvenile noble crayfish were exposed to five feeding regimes that differed in feeding frequency and food availability. Continuous growth was detected in all feeding regimes with individual increase up to 90% (wet weight) and 17% (carapace length), respectively. Morphometric parameters allowed separation of three weight‐groups or two length‐groups. During the experimental period RNA/DNA ratios showed both decrease (?17%) and increase (+35%), with superior accuracy than morphometric parameters, separating four groups. Based on RNA/DNA ratios, different feeding regimes were detected earlier, with two groups separated already after 3 weeks. RNA/DNA ratio was clearly superior to RNA per unit wet weight, as the latter failed to detect any differences between groups. In conclusion, RNA/DNA ratio is a valuable tool in nutritional studies with freshwater crayfish if overall growth is the key variable of interest. |
| |
| Keywords: | aquaculture physiological indicator intermittent feeding feeding experiment zooplankton |
|
|
