Evaluating spawning performance among captive Florida pompano Trachinotus carolinus broodstock using microsatellite‐based parentage assignment |
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| Authors: | Qian Ma Seifu Seyoum Michael D Tringali Matthew J Resley Nicole R Rhody Kevan L Main Kenneth M Leber |
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| Affiliation: | 1. Mote Marine Laboratory, Directorate of Fisheries and Aquaculture, Sarasota, FL, USA;2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China;3. Florida Fish and Wildlife Research Institute, St. Petersburg, FL, USA |
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| Abstract: | Florida pompano has been identified as a promising candidate for commercial‐scale aquaculture production, but to date, little information is available regarding captive broodstock spawning characteristics. Genetic markers were tested for their power in monitoring mating outcomes and potential in analysing heritability of rapid growth trait in Trachinotus carolinus. A total of 20 unrelated adults (10 females and 10 males) were chosen for a hormone‐induced mass spawning event. The 515 fastest growing and 485 slowest growing fish of the total 4852 offspring were considered a selected progeny stock, and fish were collected at 45 days post hatch based on their growth traits. Parentage analyses based on the 20 breeders and 1,000 selected progeny were performed using a total of nine microsatellite markers, a 100% assignment rate was achieved, and a four‐marker set was the minimum number for the parentage assignment. The effective breeding number for the selected progeny was 11 (six females and five males), among which three females and two males were predominant contributors with the total contribution of 95.8% and 94.7% respectively. The proportion of fast‐growing offspring from broodfish and each mating cross (sire/dam) was used for detecting whether variation in growth of the offspring was related to parental stocks. Results showed that three adults and their mated combination exhibited the greatest fast‐growing offspring proportion (69.73% and 55.95%). This research provided new information regarding spawning performance and parental contribution during mass spawning events; both important first steps towards developing improved management strategies for captive Florida pompano broodstock. |
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| Keywords: | Florida pompano microsatellites parentage assignment rapid growth trait reproductive success |
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