Liver histology and histomorphometry in hybrid sorubim (Pseudoplatystoma reticulatum × Pseudoplatystoma corruscans) reared on intensive fish farming |
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| Authors: | Robson Andrade Rodrigues Klaus Casaro Saturnino Carlos Eurico Fernandes |
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| Affiliation: | 1. Programa de Pós‐Gradua??o em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia – FAMEZ, Universidade Federal de Mato Grosso do Sul – UFMS, Campo Grande, MS, Brazil;2. Laboratório de Patologia Veterinária, Universidade Federal de Goiás‐ UFG, Regional Jatai – Unidade Jatobá, Jataí, GO, Brazil |
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| Abstract: | This study aimed to characterize the liver histology and histomorphometry in sorubim hybrid of different categories (nursery, growth and grow‐out) reared on fish farming. The categories were defined considering body weight (BW): nursery category (n = 5): BW = 37.06 ± 6.00 g (31.6–45.3 g); growth category (n = 5): BW = 310.40 ± 53.80 g (242.1–376.4 g) and grow‐out category (n = 5): BW = 874.28 ± 27.59 g (846.2–913.1 g). Liver fragments were processed to paraffin inclusion, and sections were stained by haematoxylin and eosin (H&E), PAS (Periodic Acid Schiff) and Perl's staining to histology, histomorphometry and density volumetric of liver structures; glycogen analysis and to detect ferric irons (Fe3+) respectively. The hepatosomatic index decreased between the categories (P < 0.01). The percentage of PAS‐positive hepatocytes in the nursery category was higher (P < 0.05) in relation to the growth and grow‐out categories. The hepatocytes from all fish were positive to Perl's staining. The density volumetric of liver structures did not differ among categories except to blood vessels were higher (P < 0.01) in the nursery and growth. The area (μm2) and perimeter (μm) of hepatocytes, and the area (μm2), perimeter (μm) and volume (μm3) of the nuclei from grow‐out fish were lower (P < 0.01) than those from the nursery and growth categories. Changes in morphometric characteristics of hepatocytes may result from metabolic changes associated with body growth surubins; therefore, these morphometric characteristics of liver tissue can be used as functional biomarkers for the assessment of fish health and nutrition status. |
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| Keywords: | functional biomarkers hepatocytes hepatic tissue stereology |
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