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Changes in fatty acids profile,monosaccharide profile and protein content during batch growth of Isochrysis galbana (T.iso)
Authors:Asma Gnouma  Irina Sadovskaya  Anissa Souissi  Khaled Sebai  Amel Medhioub  Thierry Grard  Sami Souissi
Affiliation:1. Protéomie Fonctionnelle et Potentiel Nutraceutique de la Biodiversité de la Tunisie, Faculté des Sciences de Tunis, Tunis, Tunisia;2. Equipe BPA, Institut Charles Violette EA 7394, USC Anses, Université du Littoral C?te d'Opale, Boulogne Sur Mer, France;3. UMR 8187, Laboratoire d'Océanologie et de Géosciences (LOG), CNRS, Univ. Lille, Université du Littoral C?te d'Opale, Wimereux, France;4. Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis el Manar, Tunis, Tunisia;5. Institut National des Sciences et Technologies de la Mer, Monastir, Tunisia
Abstract:To investigate the nutritional value of the marine micro‐alga Isochrysis galbana Tahitian Isochrysis strain (T.iso) as an alternative feed for aquaculture during culture age, its biochemical composition was studied under autotrophic and controlled culture conditions at different growth stages: exponential phase, early and late stationary phases and decay phase. Analysis showed that C14:0, C16:0, C16:1, C18:4 (n‐3) and C22:6 (n‐3) were the most abundant fatty acids in this alga at different growth stages. The highest values of monounsaturated fatty acids were recorded at the late stationary and the decay phases. However, the highest levels of polyunsaturated fatty acids were observed at the early stationary phase. At all growth stages, I. galbana (T.iso) contained arabinose, xylose, mannose, galactose and glucose. Glucose represented the main sugar, and its content per dry alga biomass weight increased with increasing age of the culture and reached about fourfold in the decay phase. The maximum protein content was also observed during this last phase.
Keywords:fatty acids  growth phases  Isochrysis galbana (T  iso)  monosaccharides  proteins
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