Preservation of black sharkminnow,Labeo chrysophekadion (Bleeker, 1849) spermatozoa |
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| Authors: | Phimphan Ladoktha Samorn Ponchunchoovong Charoen Udomkarn |
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| Affiliation: | 1. School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand;2. Department of Fisheries, Nakhon Ratchasima Inland Fisheries Research and Development Center, Nakhon Ratchasima, Thailand |
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| Abstract: | The objective of this study was to investigate the effects of extenders and storage time on motility, viability and fertilization of preserved black sharkminnow, Labeo chrysophekadion spermatozoa. Sperm were diluted 1:3 in one of five extenders: modified Cortland solution (MC); Hanks' balanced salt solution (HBSS); 0.9% sodium chloride (NaCl); Kurokura solution (KU); and modified extender, and undiluted sperm samples were used as control and stored at 4°C for 5 days. Motility, viability and fertilization rates were evaluated every day. After a storage time of three days, the highest motility, viability and fertilization rates (61.27 ± 2.26%, 58.60 ± 2.29% and 40.58 ± 0.57, respectively) were achieved with sperm diluted with modified extender. Motility, viability and fertilization rates decreased significantly (P < 0.05) with increasing storage time in all treatments. In addition, this study found that motility, viability and fertilization had a positive significant correlation (P < 0.01). The results indicate that isotonic extender is suitable for the short‐term preservation of black sharkminnow spermatozoa. |
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| Keywords: | preservation Labeo chrysophekadion extender spermatozoa computer‐assisted sperm motility analysis fertilization |
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