Synergistic effects of dietary vitamin E and selenomethionine on growth performance and tissue methylmercury accumulation on mercury‐induced toxicity in juvenile olive flounder,Paralichthys olivaceus (Temminck et Schlegel) |
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| Authors: | Mohammad Moniruzzaman Gunhyun Park Hyeonho Yun Seunghan Lee Youngjin Park Sungchul C Bai |
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| Affiliation: | Department of Marine Bio‐Materials and Aquaculture, Feeds & Foods Nutrition Research Center (FFNRC), Lab No. 9134B/C‐21, Pukyong National University, Busan, Korea |
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| Abstract: | An 8‐week feeding trial was conducted to evaluate the synergistic effects of dietary vitamin E and selenomethionine (SeMet) on induced methylmercury (MeHg) toxicity in juvenile olive flounder Paralichthys olivaceus. Nine semi‐purified diets were formulated to contain three different vitamin E levels as DL‐α‐tocopheryl acetate (0, 100 and 200 mg TAkg?1 diet) and three different selenium (Se) levels (0, 2 and 4 SeMet mg kg?1 diet) on the constant mercury toxicity level (20 mg MeHgkg?1 diet). Nine experimental diets, in a 32 factorial design (E0Se0, E0Se2, E0Se4, E100Se0, E100Se2, E100Se4, E200Se0, E200Se2 and E200Se4), were fed to triplicate groups of fish averaging 2.3 ± 0.04 g (mean ± SD) in the semi‐recirculation system. After 8 weeks of feeding trial, vitamin E and Se showed significant effects on weight gain (WG) of fish (P < 0.05). We found that there was a clear trend of increasing WG with elevating vitamin E and Se levels in the diets. Feed efficiency (FE), specific growth rate (SGR), protein efficiency ratio (PER) and survivability exhibited a similar trend with WG. Both antioxidants had significant interaction effects on FE and PER (P < 0.05). Methylmercury concentrations in fish muscle, liver and kidney decreases in a dose‐dependent manner as dietary vitamin E and Se levels increase. Interestingly, the most significant interactive effects of vitamin E and Se were found in liver tissue for depleting Hg concentrations (P < 0.05). These findings suggest that dietary vitamin E more than 100 mg TA kg?1 diet with 2 or 4 mg SeMet kg?1‐supplemented diets could have synergistic effects on growth and liver mercury bioaccumulation on MeHg‐induced toxicity in juvenile olive flounder. |
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| Keywords: | synergistic effect vitamin E selenomethionine methylmercury olive flounder |
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