L‐carnitine exerts a cytoprotective effect against H2O2‐induced oxidative stress in the fathead minnow muscle cell line |
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| Authors: | Qiuju Wang Xue Ju Yuke Chen Xiaoqing Dong Sha Luo Hongjian Liu Dongming Zhang |
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| Affiliation: | 1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, China;2. Fishery Technical Extension Station of Jilin Province, Changchun, China |
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| Abstract: | This study was conducted to investigate the protective effect of L‐carnitine (LC) against H2O2‐induced oxidative stress in the fathead minnow muscle cell line (FHM). The FHM cells were stimulated with 1 mM H2O2 for 1 h after LC pre‐treatment, and the cell viability and the activity and mRNA relative expression of antioxidant enzyme were measured to assess the antioxidant properties of LC. The results showed that the toxic effect of H2O2 on the viability of FHM cells was both dose‐ and time‐dependent. Furthermore, the viability of the 0.01–1 LC mM groups was significantly higher than those of the 1 mM H2O2 group. L‐carnitine protected the cells from H2O2‐induced oxidative damage, which was demonstrated by a significant reduction in the malondialdehyde and reactive oxygen species levels and increases in the intracellular total glutathione levels and the activities of total superoxide dismutase, catalase, glutathione peroxidase (GPx) and gamma‐glutamyl‐cysteine synthetase (γ‐GCS) in FHM cells pre‐treated with LC for 6 h compared with the 1 mM H2O2 group. In addition, the mRNA relative expression levels of the γ‐GCS catalytic subunit and nuclear factor nuclear factor erythroid 2‐related factor 2 were significantly higher than those of the 1 mM H2O2 group. It could be concluded that LC exerts a beneficial antioxidant effect against oxidative stress induced by H2O2 in FHM cells and that the appropriate treatment is 0.1–1 mM for 6 h in this study. |
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| Keywords: | L‐carnitine fish oxidative stress fathead minnow muscle cell line antioxidant system reactive oxygen species |
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