| 三嗪类农药复合污染物对蛋白核小球藻的联合毒性作用评估 |
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| 引用本文： | 王滔,班龙科,张瑾,卞志强,潘法康.三嗪类农药复合污染物对蛋白核小球藻的联合毒性作用评估[J].农业环境科学学报,2020,39(3):482-495. |
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| 作者姓名： | 王滔 班龙科 张瑾 卞志强 潘法康 |
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| 作者单位： | 安徽建筑大学环境与能源工程学院,安徽省水污染控制与废水资源化重点实验室,合肥230601;安徽建筑大学环境与能源工程学院,安徽省水污染控制与废水资源化重点实验室,合肥230601;安徽建筑大学环境与能源工程学院,安徽省水污染控制与废水资源化重点实验室,合肥230601;安徽建筑大学环境与能源工程学院,安徽省水污染控制与废水资源化重点实验室,合肥230601;安徽建筑大学环境与能源工程学院,安徽省水污染控制与废水资源化重点实验室,合肥230601 |
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| 基金项目： | 国家自然科学基金项目（21677001）；安徽省自然科学基金项目（1708085MB50）；安徽省质量工程项目（2016jyxm0214） |
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| 摘 要： | 为探讨复合污染的三嗪类农药对非靶标生物的联合毒性作用,以苯嗪草酮(Metamitron, Met)、草净津(Bladex, Bla)和特丁通(Terbumeton, Ter)为目标污染物,以蛋白核小球藻(Chlorella pyrenoidosa, C. pyrenoidosa)为指示生物,分别应用直接均分射线法和均匀设计射线法设计二元及三元混合物体系,采用微板毒性分析法系统测定3种农药及其混合污染物对C. pyrenoidosa的时间-浓度-效应数据,应用浓度加和(Concentration addition, CA)为标准参考模型分析混合物毒性相互作用,应用二维和三维等效图表征混合物联合毒性作用规律,并同步分析C. pyrenoidosa中叶绿素a含量变化,进一步评价农药及其混合物对小球藻的毒性效应。结果表明:农药及其混合物对C. pyrenoidosa的毒性数据可用Logit函数较好拟合,3种农药的毒性顺序为BlaTerMet;依据CA模型,二元混合物的毒性相互作用整体上呈现为加和作用向协同作用的转变,二维等效图显示3组二元混合物体系在半数效应浓度均具有较强的协同作用;农药三元混合物体系的5条射线均呈现加和作用,且不随暴露时间延长发生改变,三维等效图法与CA模型的分析结果基本一致;叶绿素a含量减少率与其浓度-效应曲线变化趋势基本一致。
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| 关 键 词： | 三嗪类农药 蛋白核小球藻 联合毒性 叶绿素a 三维等效图 |
| 收稿时间： | 2019/10/16 0:00:00 |
Evaluation of combined toxicity of triazine pesticide contaminants against Chlorella pyrenoidosa |
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| WANG Tao,BAN Long-ke,ZHANG Jin,BIAN Zhi-qiang and PAN Fa-kang.Evaluation of combined toxicity of triazine pesticide contaminants against Chlorella pyrenoidosa[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2020,39(3):482-495. |
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| Authors: | WANG Tao BAN Long-ke ZHANG Jin BIAN Zhi-qiang and PAN Fa-kang |
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| Affiliation: | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China,Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China,Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China,Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China and Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China |
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| Abstract: | In order to explore the toxicity of pesticide mixtures to non-target organisms, three triazine pesticides:metamitron(Met), bladex (Bla), and terbumeton(Ter)being as research objects and Chlorella pyrenoidosa(C. pyrenoidosa)as a test organism, direct equipartition ray and uniform design ray procedures were used to design binary and ternary mixture systems, respectively. The time-concentration-effect curves of triazine pesticide contaminants on C. pyrenoidosa were determined by using the microplate toxicity analysis method. Concentration addition (CA)was used as a standard reference model to analyze the toxicity interaction within mixture systems. Two-dimensional isobologram and three-dimensional isobologram were applied to characterize combined toxicity interactions of mixtures. The change of chlorophyll a content in C. pyrenoidosa was analyzed to further evaluate the toxic effects of pesticides and their mixtures on C. pyrenoidosa. The results show that the Logit function can effectively describe the toxicity of pesticides and their mixtures toward C. pyrenoidosa, the toxicity order of the three triazines is as follows:Met > Bla > Ter. Based on the results predicted by CA model, toxicity interaction within binary mixtures changes from additive action to synergism, two-dimensional isobolograms indicate that the three binary mixture systems all show strong synergism at the median effective concentration. Five rays of ternary mixture systems all exhibit additive action which remain unchanged with the lengthening of exposure time. The results predicted by CA model are the same as those of three-dimensional isobolograms. The reduction rate of chlorophyll a content is consistent with the changing trends of the pesticides'' concentration-response curves. |
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| Keywords: | triazine pesticides Chlorella pyrenoidosa combined toxicity chlorophyll a three-dimensional isobologram |
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