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水体Cu2+对三疣梭子蟹主要组织ROS含量和抗氧化能力的影响
引用本文:王丽,韩艳楠,金珊,赵青松,陈寅儿,王春琳.水体Cu2+对三疣梭子蟹主要组织ROS含量和抗氧化能力的影响[J].农业环境科学学报,2015,34(7):1261-1268.
作者姓名:王丽  韩艳楠  金珊  赵青松  陈寅儿  王春琳
作者单位:宁波大学 海洋学院, 浙江 宁波 315211,宁波大学 海洋学院, 浙江 宁波 315211,宁波大学 海洋学院, 浙江 宁波 315211,宁波大学 海洋学院, 浙江 宁波 315211,宁波大学 海洋学院, 浙江 宁波 315211,宁波大学 海洋学院, 浙江 宁波 315211
基金项目:浙江省教育厅项目(Y201224126);宁波市科技创新团队(2011B81003);宁波大学学科项目(XKL11093)
摘    要:为探究水体Cu2+对三疣梭子蟹主要组织氧自由基水平和抗氧化能力的影响,采用生态学单因子梯度试验方法,研究了不同浓度水体Cu2+(0.04、0.4、2、4 mg·L-1)胁迫下三疣梭子蟹鳃、肝胰腺、肌肉组织中的活性氧(ROS)含量、超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)的变化。结果表明:与对照组相比,低浓度水体Cu2+(0.04、0.4 mg·L-1)对三疣梭子蟹鳃、肝胰腺和肌肉组织ROS含量没有显著影响,而高浓度水体Cu2+(2、4 mg·L-1)会导致鳃、肝胰腺和肌肉组织ROS含量显著升高,且升高的幅度与Cu2+浓度成正比。低浓度水体Cu2+(0.04、0.4 mg·L-1)对三疣梭子蟹鳃、肝胰腺和肌肉组织的SOD活性和T-AOC均具有诱导作用,随实验时间的延长,SOD活性和T-AOC呈峰值变化;而高浓度水体Cu2+(2、4 mg·L-1)短时间内对梭子蟹的鳃、肝胰腺、肌肉组织的SOD活性及鳃T-AOC具有诱导作用,但对肝胰腺和肌肉T-AOC却有明显的抑制作用,在实验时间内两种组织T-AOC始终低于对照组,并呈逐渐下降趋势。由此说明低于0.4 mg·L-1的水体Cu2+在三疣梭子蟹抗氧化解毒能力的可控范围,但高于2 mg·L-1的水体Cu2+会对三疣梭子蟹主要组织细胞造成损伤,使之抗氧化解毒能力下降。抗氧化酶和非酶抗氧化物在三疣梭子蟹抵御Cu2+胁迫中共同发挥作用,三种组织SOD和T-AOC表现出明显的时间和剂量效应。

关 键 词:三疣梭子蟹  Cu2+  活性氧  超氧化物歧化酶  总抗氧化能力
收稿时间:2015/1/27 0:00:00

Effects of Aqueous Copper on Reactive Oxygen Species Content and Anti-oxidation Capacity of Major Tissues in Portunus trituberculatus
WANG Li,HAN Yan-nan,JIN Shan,Zhao Qing-song,CHEN Yin-er and WANG Chun-lin.Effects of Aqueous Copper on Reactive Oxygen Species Content and Anti-oxidation Capacity of Major Tissues in Portunus trituberculatus[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2015,34(7):1261-1268.
Authors:WANG Li  HAN Yan-nan  JIN Shan  Zhao Qing-song  CHEN Yin-er and WANG Chun-lin
Affiliation:School of Marine, Ningbo University, Ningbo 315211, China,School of Marine, Ningbo University, Ningbo 315211, China,School of Marine, Ningbo University, Ningbo 315211, China,School of Marine, Ningbo University, Ningbo 315211, China,School of Marine, Ningbo University, Ningbo 315211, China and School of Marine, Ningbo University, Ningbo 315211, China
Abstract:High levels of copper in water can affect aquacultural production. Here an experiment was designed to examine the responses of reactive oxygen species(ROS), superoxide dismutase(SOD) and total anti-oxidation capacity(T-AOC) in gill, hepatopancreas and muscle of Portunus trituberculatus exposed to different concentrations of Cu2+(0, 0.04, 0.4, 2 and 4 mg·L-1) using single factor gradient method of ecology. The ROS level increased with elevated concentrations of Cu2+(2 mg·L-1 and 4 mg·L-1), but was no significant differences(P>0.05)between 0.04 and 0.4 mg·L-1. Compared with control, SOD and T-AOC activities in gill, hepatopancreas and muscle were induced by low concentrations of Cu2+(0.04 and 0.4 mg·L-1), and displayed an inverted V-shape over the experimental period. Furthermore, the activities of SOD and T-AOC in gill, hepatopancreas and muscle showed obvious relationships between concentration-time effects and time-effect. Under high concentrations of Cu2+(2 and 4 mg·L-1), the activity of SOD in gill, hepatopancreas and muscle and the activity of T-AOC in gill were induced during the initial 24 h, but activity of T-AOC in hepatopancreas and muscle were inhibited at the beginning and decreased markedly over time. The present results suggest that exposure to low(<0.04 mg·L-1) and short-term exposure to high Cu2+ concentrations(>2 mg·L-1) would not cause significant oxidative damages, but enhance the antioxidant defense system. Under extended exposure to high Cu2+, however, oxidative damage was obviously increased, decreasing the antioxidation capacity. Antioxidant enzyme and non-enzymatic antioxidants could jointly play a role in the defense system.
Keywords:Portunus trituberculatus  Cu2+  ROS  SOD  T-AOC
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