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不同产地苦荞籽粒中多酚的组成、分布及抗氧化性比较
引用本文:刘琴,张薇娜,朱媛媛,胡秋辉.不同产地苦荞籽粒中多酚的组成、分布及抗氧化性比较[J].中国农业科学,2014,47(14):2840-2852.
作者姓名:刘琴  张薇娜  朱媛媛  胡秋辉
作者单位:南京财经大学食品科学与工程学院/江苏省粮油品质控制及深加工技术重点实验室;
基金项目:江苏省高校自然科学研究重大项目(11KJA550001);2010江苏省高校“青蓝工程”科技创新团队资助项目
摘    要:【目的】对不同产地的不同品种苦荞中多酚在壳、麸皮和粉中的含量以及多酚含量与抗氧化性的相关性进行比较研究,为苦荞的育种及深度加工利用提供科学依据。【方法】用Folin-酚法测定来自3个省份的17个品种苦荞的壳、麸皮、粉及全谷中的总酚含量,用液相色谱与质谱联用(HPLC/MS)结合串联质谱法(MS/MS)对多酚的成分进行鉴定和定量分析,并用DPPH和ORAC法对不同样品的多酚提取物的抗氧化性进行比较。【结果】Folin-酚法和HPLC法测定结果均表明,苦荞麸皮中的总酚含量最高,按HPLC法测定结果,17个品种苦荞麸皮中总酚平均含量为4 410.23 mg/100 g,分别是壳和粉中总酚平均含量的4.8倍和15.5倍。在所有样品中共鉴定出8个多酚组分,其中芦丁为主要成分,在壳、麸皮和粉中的含量分别为342.55—1758.06 mg/100 g、2 653.84—5 488.55和148.23—542.68 mg/100 g,分别占壳、麸皮和粉的总酚含量的82.13%—90.16%、87.71%—92.09%和80.85%—86.53%。绿原酸只在壳中被检出,而金丝桃苷主要存在于壳和麸皮中。不同产区的不同品种苦荞中多酚的分布及含量差异显著,其中,云南产的苦荞壳中的总酚含量显著低于四川和陕西产的苦荞壳中总酚含量,但麸皮中的总酚平均含量却高于四川产苦荞麸皮中的平均含量,与陕西产苦荞接近。DPPH和ORAC抗氧化性结果均显示,所有苦荞样品麸皮的抗氧化性最高,粉的抗氧化性最低;总的来说,陕西产苦荞的壳、麸皮和粉均具有最高抗氧化性,云南产的苦荞壳和粉的抗氧活性最低,但全谷具有最高的抗氧化活性。【结论】不同产地不同品种苦荞的多酚含量分布及抗氧化活性显著不同,所有样品的DPPH和ORAC抗氧化值均与总酚含量正相关。

关 键 词:苦荞    多酚组成    液质联用    多酚分布    抗氧化性
收稿时间:2014-02-25

Comparison of the Constitutions,Distribution, and Antioxidant Activities of Polyphenols from Different Varieties of Tartary Buckwheat Seed Produced from Different Regions of China
LIU Qin,ZHANG Wei-Na,ZHU Yuan-Yuan,HU Qiu-Hui.Comparison of the Constitutions,Distribution, and Antioxidant Activities of Polyphenols from Different Varieties of Tartary Buckwheat Seed Produced from Different Regions of China[J].Scientia Agricultura Sinica,2014,47(14):2840-2852.
Authors:LIU Qin  ZHANG Wei-Na  ZHU Yuan-Yuan  HU Qiu-Hui
Affiliation:School of Food Science and Engineering, Nanjing University of Finance and Economics/Jiangsu Key Laboratory for Grain and Oil Quality Control and Further Technology, Nanjing 210023
Abstract:【Objective】 The constitution, content and distribution of polyphenols in hull, bran, flour and whole grain of different varieties of tartary buckwheats from different regions of China were studied. The antioxidant activities of polyphenol extracts from different parts and samples of tartary buckwheat were compared. The aim of the study is to obtain the scientific data for improvement of tartary buckwheat breeding and further processing. 【Method】 Total phenolic contents (TPCs) of hull, bran, flour and whole grain of 17 varieties of tartary buckwheat were firstly compared by Folin–Ciocalteu reagent method. The polyphenol constitutions and individual phenolics content were analyzed by HPLC coupled with mass spectrometry (HPLC/MS) and tandem MS (MS/MS). The antioxidant activities of different polyphenol extracts were compared by DPPH and ORAC methods. 【Result】 Both Folin–Ciocalteu reagent and HPLC methods showed that tartary buckwheat bran had the highest TPC. Based on HPLC results, the average TPC in bran of all samples was 4 410.23 mg/100 g, which was 4.8 times of average TPC in hull and 15.5 times of average TPC in buckwheat flour. There were eight polyphenol species identified by HPLC/MS and MS/MS, among them Rutin was the major species. The contents of rutin in hull, bran and flour were 342.55-1 758.06 mg/100 g, 2 653.84-5 488.55 and 148.23-542.68 mg/100 g, which accounted for 82.13%-90.16%, 87.71%-92.09% and 80.85%-86.53% of TPC in hull, bran, and flour, respectively. Chlorogenic acid was only detected in hull samples. While, hyperoside mainly occur in hull and bran, and sinapic acid was firstly detected in tartary buckwheat at very low concentration. Polyphenol distribution and contents in buckwheats from different regions were significantly different. The TPCs in hulls from Yunnan were significantly lower than those from Shaanxi and Sichuan. However, the average TPC in brans of buckwheats from Yunnan was higher than those from Sichuan, and closed to those from Shaanxi. The whole grains from Yunnan had the highest TPC and antioxidant activities. The DPPH and ORAC results showed that for all samples, brans had the highest antioxidant activities, while flours had the lowest antioxidant activities. Overall, the buckwheat samples from Shaanxi had the highest DPPH and ORCA values no matter for hull, bran and flour. The hulls and flours from Yunnan had the lowest value in both DPPH and ORAC values, but had the highest antioxidant capability for whole grains. 【Conclusion】HPLC coupled with mass spectrometry is very powerful in studying the polyphenol in plants. Results of this study showed that the polyphenol distribution and contents in tartary buckwheat from different regions of China were significantly different. And the TPCs positively correlated with the antioxidant activities.
Keywords:tartary buckwheat  polyphenol distribution  polyphenol constitution  HPLC/MS  antioxidant activity
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