| 中国小麦微核心种质资源PPO基因的等位变异 |
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| 引用本文： | 王晓波,马传喜,司红起,乔玉强,何贤芳.中国小麦微核心种质资源PPO基因的等位变异[J].中国农业科学,2009,42(1):28-35. |
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| 作者姓名： | 王晓波 马传喜 司红起 乔玉强 何贤芳 |
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| 作者单位： | 安徽农业大学农学院,合肥,230036 |
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| 基金项目： | 国家科技支撑计划，农业部引进国际先进农业科学技术项目，公益性行业(农业)科研专项基金 |
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| 摘 要： | 【目的】小麦籽粒中的多酚氧化酶活性与面制食品的褐变密切相关。研究中国小麦微核心种质资源PPO活性的变异及其基因型的分布规律，为种质资源的合理利用以及中国面制食品外观品质的改良提供依据。【方法】以251份遗传多样性丰富的中国小麦微核心种质资源为试验材料，2004—2005年度种植于两个地点（安徽合肥、凤阳），采用生化和特异引物PCR扩增的方法对其PPO活性及基因型进行鉴定。【结果】中国小麦微核心种质资源品种间PPO活性差异明显，并且低PPO活性的品种居多;基因型、地点及其互作对PPO活性的影响达到1%显著水平。利用控制小麦PPO活性两个主效基因位点的特异PCR引物扩增结果表明，中国小麦微核心种植资源中共检测出PPO-2Aa1/PPO-2Da2（a1a2）、PPO-2Aa1/PPO-2Db2（a1b2）、PPO-2Ab1/PPO-2Da2（b1a2）和PPO-2Ab1/PPO-2Db2（b1b2）4种标记基因型，标记基因型间PPO活性均值的大小顺序为：a1a2< a1b2< b1a2
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| 关 键 词： | 普通小麦 微核心种质资源 多酚氧化酶 等位变异 |
| 收稿时间： | 2008-1-10 |
Allelic Variation of PPO Genes in Chinese Wheat Micro-Core Collections |
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| WANG Xiao-bo,MA Chuan-xi,SI Hong-qi,QIAO Yu-qiang,HE Xian-fang.Allelic Variation of PPO Genes in Chinese Wheat Micro-Core Collections[J].Scientia Agricultura Sinica,2009,42(1):28-35. |
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| Authors: | WANG Xiao-bo MA Chuan-xi SI Hong-qi QIAO Yu-qiang HE Xian-fang |
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| Affiliation: | College of Agronomy, Anhui Agricultural University |
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| Abstract: | 【Objective】 Grain polyphenol oxidase (PPO) activity is highly related to the brown discoloration of wheat based end-products. Studying on the variation of PPO activity and the distribution of allelic genes in Chinese wheat micro-core collections could take good advantage of germplasm and improve the appearance quality of wheat flour food. 【Method】 In this study the variation of PPO activity and the distribution of PPO allelic genes were detected by the method of biochemistry and gene-based markers in 251 Chinese wheat micro-core collections growning in Hefei and Fengyang in 2004-2005 crop season. 【Result】 The results showed that most cultivars were with low PPO activity and the rate of varieties with PPO activity below 200 was 47.01% and the variation of PPO activity was affected by genotype and locations and interaction between them. Four genotypes, PPO-2Aa1/PPO-2Da2 (a1a2), PPO-2Aa1/PPO-2Db1 (a1b1), PPO-2Aa1/PPO-2Db2 (a1b2), and PPO-2Ab1/PPO-2Db2 (b1b2), were detected in Chinese wheat micro-core collections. Cultivars with the genotype a1a2 have the lowest PPO activity while the cultirars with the genotype b1b2 have the highest, with genotype a1b2 and genotype b1a2 have the intermediate. The effect of interaction between marker genotypes and locations on PPO activity was not significant (P＜0.05). The frequency of PPO allelic genes on two wheat chromosomes 2A and 2D was different. The frequency of allelic genes 2Aa1 and 2Ab1 on chromosome 2A was most alike, while the frequency of 2Da2 was almost four times than that of 2Db2 on chromosome 2D. The effect of haplotype of PPO genes on the same chromosome on PPO activity was PPO-2Aa1＜PPO-2Ab1 and PPO-2Da2＜PPO-2Db2. The mean value of PPO activity of cultivars from South China Winter Wheat Region was significantly (P＜0.01) lower than that of cultivars from the three spring wheat regions and cultivars imported from aboard. Cultivars from the five winter wheat regions gave significantly (P＜0.01) lower PPO activity than cultivars from the three spring wheat regions of China. PPO activity of landrace was significantly (P＜0.01) lower than that of bred varieties and cultivars imported from aboard. The PPO activity and genotypes of primal parents in Chinese wheat cultivars was also analysized in this study. 【Conclusion】Variation of PPO activity in Chinese wheat micro-core collections is obvious, and the allelic variation of PPO genes induced a significant effect on grain PPO activity. The distribution of wheat grain PPO activity and genotypes have a close relationship with wheat regions and wheat primary parents. |
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| Keywords: | common wheat (Triticum aestivum micro-core collections polyphenol oxidase allelic variation |
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