| 基因枪法转基因水稻后代农艺性状的表现 |
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| 引用本文： | 唐祚舜,李良材,田文忠.基因枪法转基因水稻后代农艺性状的表现[J].中国农业科学,2001,34(6):581-586. |
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| 作者姓名： | 唐祚舜 李良材 田文忠 |
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| 作者单位： | 中国科学院遗传与发育生物学研究所,北京,100101 |
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| 摘 要： | 通过基因枪转化法，将潮霉素磷酸转移酶基因（ｈｐｔ）导入粳稻品种７７１７０和中花９号，获得转化再生植株，选其中７个结实较好的株系为试验材料，对其自交后代（Ｔ１ 和Ｔ２）的株高、穗数、穗长、穗粒数、结实率和千粒重等６个农艺性状进行调查分析。结果显示，转基因植株后代６个农艺性状都不同程度地发生了变异，ＳＧ－１的千粒重变异较大，ＳＧ－２穗数变异较大，ＳＧ－３－１结实率变异较大，ＳＧ－３－２在６个性状上都有较大的变异，ＳＧ－１５主要是在株高、穗长、穗粒数以及穗数和千粒重５个性状上有较大变异。产生这些变异的主要原因是在组织培养过程中发生了体细胞无性系变异，导入外源基因并没有直接影响农艺性状的表达。在Ｔ１和Ｔ２代中，千粒重和结实率的表现基本一致，而株高、穗数、穗长和穗粒数的遗传表现还不够稳定。因此，在Ｔ１代选育时，应注重选择千粒重和结实率表现好的株系，而对本研究涉及的其它性状可不作严格选择。
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| 关 键 词： | 水稻 转基因植物 农艺性状 潮霉素磷酸转移酶基因(hpt) |
| 文章编号： | 0578-1752(2001)06-0581-06 |
| 修稿时间： | 2001年1月15日 |
Agronomic Traits in Progeny of Transgenic Rice Mediated by Biolistic Bombardment |
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| TANG Zuo-shun,LI Liang-cai,TIAN Wen-zhong.Agronomic Traits in Progeny of Transgenic Rice Mediated by Biolistic Bombardment[J].Scientia Agricultura Sinica,2001,34(6):581-586. |
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| Authors: | TANG Zuo-shun LI Liang-cai TIAN Wen-zhong |
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| Abstract: | Japonica rice cv 77-170 and ZH9 were transformed with pBI222 carrying hygromycin phos- photransferase gene(hpt) by using biolistic bombardment method, and regenerated plants were obtained. Seven seed-setting regenerants(SG-1, SG-2, SG-3-1, SG-3-2, SG-15 was derived from 77-170, and YW2- I-6, YW,-5-8 was derived from ZH9) were selected as materials to analyse the variation of 6 agronomic traits(plant height, number of panicle, panicle length, number of spikelets per panicle, seed-setting rate and grain weight ) in their T1 and T2 generation. Different variation of 6 agronomic traits were observed in the progenies of different transgenic rice plants. These changes mainly due to the somaclonal variation, and introduction of hPt did not directly affect the performance of agronomic traits in the progeny of trans- genic plants. The variation at seed-setting rate and grain weight were basically similar between T1 and T2 generation,so the plant lines which performed better on the two traits should be selected in T1 generation, and the traits of plant height, number of panicle, panicle length and number of spikelets per panicle may not be selected in early generation for their unstable inheritance from T, generation to T2. |
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| Keywords: | Rice Transgenic plant Agronomic trait Hygromycin phosphotransferase gene(hpt) |
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