| 油菜籽含油量和蛋白质含量的种子胚与母体植株QTL定位 |
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| 引用本文： | 许剑锋,龙艳,吴建国,赵志刚,徐海明,温娟,孟金陵,石春海.油菜籽含油量和蛋白质含量的种子胚与母体植株QTL定位[J].中国农业科学,2014,47(8):1471-1480. |
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| 作者姓名： | 许剑锋 龙艳 吴建国 赵志刚 徐海明 温娟 孟金陵 石春海 |
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| 作者单位： | 1、浙江大学农业与生物技术学院，杭州 310058；
2、华中农业大学植物科学技术学院，武汉 430070；
3、浙江农林大学农业与食品科学学院，浙江临安 311300；
4、青海省农林科学院，西宁 810016 |
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| 基金项目： | 浙江省作物种质资源重点实验室项目;教育部《高等学校骨干教师资助计划》项目(J20000052);浙江省“151人才工程”第一层次培养资助项目 |
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| 摘 要： | 【目的】利用甘蓝型油菜TN DH群体分别与双亲Tapidor和Ningyou7回交构建的BC1F1 1和BC1F1 2两个群体，分析油菜籽含油量和蛋白质含量的种子胚和母体植株两套不同核基因组的QTL及其遗传效应，以明确QTL在不同遗传体系中的分布状况以及连锁的分子标记，研究环境互作效应对不同遗传体系QTL定位的影响，探讨相应品质性状分子标记辅助选择的最优策略和方法。【方法】按照常规田间试验方法种植202个TN DH群体材料与双亲，采用2年、2次重复、随机区组试验设计，开花时通过双向回交构建BC1F1 1和BC1F1 2两个群体，收获双亲和回交群体的种子。利用可分析含油量和蛋白质含量的近红外分析模型和方法测定油菜籽含油量和蛋白质含量。结合甘蓝型油菜分子标记连锁遗传图谱以及新创建的双子叶作物种子品质性状两套遗传体系的QTL定位方法和作图软件，对不同年份BC1F1 1和BC1F1 2油菜籽含油量和蛋白质含量进行QTL定位分析。【结果】共检测到7个与油菜籽含油量和蛋白质含量相关的QTL，分布在A1、A4、A6、A7、C2和C5连锁群上，其中，4个与含油量相关的QTL和3个控制蛋白质含量的QTL对表型的总贡献率分别为49.1%和59.6%。检测到的QTL均具有极显著的胚加性主效应和母体加性主效应，其中4个QTL具有显著或极显著的胚显性主效应、2个与含油量相关的QTL具有极显著的环境互作效应。qOC-6-3和qPC-4-1作为控制含油量和蛋白质含量的重要QTL，分别能解释36.3%和37.9%的表型变异；而qOC-4-2和qPC-4-1均被定位在甘蓝型油菜A4连锁群相同的位点上，位于分子标记HS-K02-2和HBR094之间，QTL峰值位置为18.5 cM，置信区间为17.5-19.4 cM。【结论】甘蓝型油菜籽含油量和蛋白质含量的表现会同时受到种子胚和母体植株两套不同遗传体系核基因组QTL表达效应的影响，其中环境互作效应对含油量表现的作用更为明显，而控制蛋白质含量表现的QTL在不同环境条件下的表达较为稳定。在A6和A4连锁群上检测到的qOC-6-3和qPC-4-1是2个控制含油量和蛋白质含量的主效QTL，同时2个控制蛋白质含量的QTL尚未见报道。
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| 关 键 词： | 甘蓝型油菜 含油量 蛋白质含量 QTL定位 遗传主效应 QTL与环境互作效应 |
| 收稿时间： | 2013-11-05 |
QTL Mapping Based on Embryo and Maternal Genetic Systems for Oil and Protein Contents in Rapeseed (Brassica napus L.) |
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| XU Jian-Feng-,LONG Yan-,WU Jian-Guo-,ZHAO Zhi-Gang-,XU Hai-Ming-,WEN Juan-,MENG Jin-Ling-,SHI Chun-Hai-.QTL Mapping Based on Embryo and Maternal Genetic Systems for Oil and Protein Contents in Rapeseed (Brassica napus L.)[J].Scientia Agricultura Sinica,2014,47(8):1471-1480. |
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| Authors: | XU Jian-Feng- LONG Yan- WU Jian-Guo- ZHAO Zhi-Gang- XU Hai-Ming- WEN Juan- MENG Jin-Ling- SHI Chun-Hai- |
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| Affiliation: | 1、College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058;
2、College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070;
3、School of Agricultural and Food Science, Zhejiang A&F University,Lin’an 311300, Zhejiang;
4、Qinghai Academy of Agricultural and Forestry Sciences, Xining 810016 |
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| Abstract: | 【Objective】 In present study, BC1F1 1 and BC1F1 2 populations constructed from Brassica napus genetic population TN DH and parents Tapidor and Ningyou7 were used to analyze the distribution and linkage markers of QTLs with embryo genetic effects, maternal genetic main effects, and their QTL × environment interaction effects on oil and protein contents in rapeseed, as well as to study the influence of environmental interaction on QTL mapped on different genetic systems and explore the optimal strategies and methods for improvement of these two quality traits in marker-assisted selection. 【Method】 A total of 202 TN DH lines and parents were planted in accordance with the general method for field experiments, based on randomized block design with two replications for two years. At flowering stage, BC1F1 1 and BC1F1 2 populations were constructed by double cross between 202 TN DH lines and parents, and the mature seeds of backcross population and parents were harvested for determining the oil and protein contents in rapeseed. Near infrared reflectance calibration models and methods were used for the measurement of oil and protein contents. A genetic map and a newly developed QTL mapping software and methods considering two different genetic systems for quality traits of dicotyledonous crop seeds were used to detect QTLs for oil and protein contents with the data from BC1F1 1 and BC1F1 2 populations in different years. 【Result】 A total of seven QTLs relevant to oil and protein contents in rapeseed were distributed in A1, A4, A6, A7, C2 and C5 linkage groups, in which four QTLs associated with oil content were detected as well as three QTLs for protein content, with total phenotypic contributions of 49.1% and 59.6%, respectively. All QTLs showed extremely significant embryo additive main effects and maternal additive main effects, in which four QTLs were found to have significant or extremely significant embryo dominant main effects, and two QTLs for oil content had significant environmental interaction effects. qOC-6-3 and qPC-4-1 were detected as the important QTLs with larger genetic effects, which could explain 36.3% and 37.9% of the phenotypic variation in oil content and protein content, respectively. qOC-4-2 and qPC-4-1 were co-localized in the A4 linkage group between molecular markers HS-K02-2 and HBR094 with the confidence interval of 17.5-19.4 cM. 【Conclusion】 The results showed that oil content and protein content in rapeseed could be controlled by the expression of QTLs located in different genetic systems of seed embryo and maternal plant, and environment interaction effects play more significant roles on the performance of oil content while the QTL expression of protein content was relatively stable in different environmental conditions. In addition, qOC-6-3 detected on A6 linkage group and qPC-4-1 on A4 linkage group are major QTLs for oil and protein contents，respectively, and three QTLs for protein content in present experiment have not been reported ever since. |
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| Keywords: | Brassica napus L oil content protein content quantitative trait locus (QTL) genetic main effect QTL ×environment interaction effect |
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