| 黍稷种质资源芽、苗期耐中性混合盐胁迫评价与耐盐生理机制研究 |
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| 引用本文： | 刘敏轩,张宗文,吴斌,陆平.黍稷种质资源芽、苗期耐中性混合盐胁迫评价与耐盐生理机制研究[J].中国农业科学,2012,45(18):3733-3743. |
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| 作者姓名： | 刘敏轩 张宗文 吴斌 陆平 |
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| 作者单位： | 1.中国农业科学院作物科学研究所，北京100081 |
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| 基金项目： | 农业部谷子糜子产业体系(CARS-07-12[1].5-A1);农业部作物种质资源保护项目(NB2012-2130135-25-06-1) |
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| 摘 要： | 【目的】评价黍稷资源对中性混合盐胁迫的耐受性，研究不同耐性黍稷在混合盐胁迫下的生理应答机制，挖掘耐中性混合盐胁迫的黍稷资源并探讨黍稷芽苗期耐盐鉴定的合适鉴定指标。【方法】试验设置4个不同浓度的中性混合盐（NaCl﹕Na2SO4=1﹕1）溶液进行胁迫处理（CK：0 mmol?L-1，T1：80 mmol?L-1，T2：160 mmol?L-1，T3：240 mmol?L-1），对16份黍稷材料的发芽率、复萌率以及苗期正常种苗率、苗高、苗重等生长参数进行测定，采用模糊数学隶属函数法计算各指标的隶属值，通过比较各指标隶属值总平均值的大小来确定各材料耐盐性的强弱。【结果】随着混合盐浓度的增加，各参试材料的发芽率、复萌率以及苗期的存活率均呈现下降趋势，但苗高、根长以及苗鲜重和根鲜重随着盐浓度的增加呈现出先升高后降低的趋势，在80 mmol?L-1混合盐胁迫下多数材料的苗高、根长以及苗重和根重均高于对照。模糊数学隶属函数法排序结果显示中卫大黄糜、宁糜4号以及64-4129排在前三位，具有较强耐盐性，而巴盟573黄糜子、伊盟一点棕以及瓦灰软糜排在后三位，其耐盐性较差。耐盐材料的游离脯氨酸含量以及根中Na+含量和Na+/K+随着盐胁迫浓度的增加而增加，增加幅度要显著大于耐盐性较差的材料；与此相反，耐盐性较强的材料茎叶Na+含量和Na+/K+增加幅度小于耐盐性较差的材料。【结论】16份黍稷种质材料在对中性盐胁迫的耐受性上存在显著差异，通过模糊数学隶属函数法综合芽苗期各指标筛选出的耐盐材料可进一步用于耐盐育种与耐盐基因挖掘研究。
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| 关 键 词： | 黍稷 中性混合盐胁迫 耐盐性 耐盐生理应答 |
| 收稿时间： | 2012-05-02 |
Evaluation of Mixed Salt-Tolerance at Germination Stage and Seedling Stage and the Related Physiological Characteristics of Panicum miliaceum L. |
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| LIU Min-xuan,ZHANG Zong-wen,WU Bin,LU Ping.Evaluation of Mixed Salt-Tolerance at Germination Stage and Seedling Stage and the Related Physiological Characteristics of Panicum miliaceum L.[J].Scientia Agricultura Sinica,2012,45(18):3733-3743. |
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| Authors: | LIU Min-xuan ZHANG Zong-wen WU Bin LU Ping |
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| Affiliation: | (Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081) |
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| Abstract: | 【Objective】 Salt-tolerance and physiological responses of broomcorn millet (Panicum miliaceum L.) were evaluated under a range of NaCl+Na2SO4 concentrations to explore and utilize the germplasm with high salt-tolerance and to provide physiological indicators for identifying salt-tolerance at seedling stage. 【Method】 The growth parameters of 16 genotypes of P. miliaceum L. at germination and seedling stages were measured under various salt stresses, and the genotypes were sorted out using the method of membership function. The physiological characteristics of the genotypes in terms of concentration of proline and Na+/K+ were analyzed.【Result】It was shown that the relative germination value, survival seedling number, shoot length, root length and biomass of above and underground all decreased with the increase of salt concentration, although genotypes differed in the degrees of response to salt stress. Based on the membership function on the means of parameters at germination and seedling stages for each level of salt stress, the 16 genotypes were finally ranked with Zhongwei Dahuangmi, Ningmi No4 and 64-4129 as the most tolerant genotypes, and Bameng 573 Huangmizi, Yimen Yidianzong and Wahui Ruanmi as the least tolerant ones among all tested materials. Proline content in shoot increased in response to increased salt stress, with Zhongwei Dahuangmi and Ningmi No.4 had significantly higher proline content, while lower relative Na+/K+ than the others when salt concentrations at 160 and 200 mmol.L-1 (P＜0.05). 【Conclusion】Remarkable variations in salt tolerance were detected among the 16 genotypes of P. miliaceum L., which have provided a basis for the utilization of salt tolerant broomcorn millet genetic resources. |
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| Keywords: | Panicum miliaceum L mixed salt stress salinity tolerance physiological response |
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