| 玉米秸秆高效腐解复合菌系CSS-1的选育及其组成分析 |
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| 引用本文： | 刘尧,李力,李俊,关大伟,姜昕,沈德龙,杜秉海.玉米秸秆高效腐解复合菌系CSS-1的选育及其组成分析[J].中国农业科学,2010,43(21):4437-4446. |
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| 作者姓名： | 刘尧 李力 李俊 关大伟 姜昕 沈德龙 杜秉海 |
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| 作者单位： | (山东农业大学生命科学学院) |
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| 基金项目： | 国家科技支撑计划项目，中央级公益性科研院所科研业务费专项 |
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| 摘 要： | 【目的】选育在常温(22℃)下对玉米秸秆具有高效稳定腐解功能的微生物复合菌系,明确其菌群组成,以促进选育的复合菌系在中国北方原位还田玉米秸秆中的应用。【方法】采用限制性培养与温度梯度诱导相结合的方法,以山西、山东、北京等省(市)玉米秸秆连续多年还田的土壤样品为菌源,以秸秆失重率、C/N及CMC酶活为筛选指标,获得高效稳定的玉米秸秆腐解复合菌系CSS-1;比较复合菌系不同继代腐解秸秆的失重率、CMC酶活、木聚糖酶活和PCR-DGGE的菌群监测动态变化,评价CSS-1的功能及其组成稳定性;采用克隆文库法,解析复合菌系CSS-1的主要菌群组成。【结果】CSS-1处理玉米秸秆的CMC平均酶活和最高酶活,比目前广泛应用的秸秆腐解菌剂A分别提高了82%和79%;CSS-1处理的秸秆失重率比菌剂A和空白对照分别提高了62.62%和173.65%;CSS-1不同继代腐解秸秆功能及其菌系组成监测表明该复合菌系的稳定性;克隆文库方法显示CSS-1优势菌群由多种具有纤维素与半纤维素降解功能的细菌和真菌构成,其细菌菌群分别归属Enterobacter、Cellulomonas、Streptomyces、Bacillaceae、Pantoe、Cellvibrio6个属,真菌菌群归属Trichoderma、Gibberella2个属。【结论】获得了在中国北方地区常温条件下高效稳定腐解玉米秸秆的复合菌系CSS-1,可研发利用该菌系促进田间原位还田玉米秸秆的快速腐解。
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| 关 键 词： | 玉米秸秆" target="_blank">face="Verdana">玉米秸秆 微生物复合菌系 腐解 组成分析 克隆文库 |
| 收稿时间： | 2010-04-02; |
Construction and Composition Analysis of the Complex Microbial System CSS-1 of High Decomposition Efficiency for Corn Stalks |
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| LIU Yao,LI Li,LI Jun,GUAN Da-wei,JIANG Xin,SHEN De-long,DU Bing-hai.Construction and Composition Analysis of the Complex Microbial System CSS-1 of High Decomposition Efficiency for Corn Stalks[J].Scientia Agricultura Sinica,2010,43(21):4437-4446. |
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| Authors: | LIU Yao LI Li LI Jun GUAN Da-wei JIANG Xin SHEN De-long DU Bing-hai |
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| Affiliation: | (College of Life Sciences, Shandong Agricultural University)
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| Abstract: | 【Objective】 In order to accelerate decay of cornstalks returned to fields in Northern China, the complex microbial system CSS-1 with high efficiency and stable degrading ability for corn stalks was constructed and its microbial composition was analyzed. 【Method】 The CSS-1 was domesticated from the soils which had been treated with cornstalks for a long time in Shanxi, Shandong and Beijing using the methods of nutrition-restricted cultivation combined with gradient-temperature induction. Its decomposition ability was determined by weight-loss percentage, C/N and CMC enzyme activity of the treated corn stalks. The stability of CSS-1 was confirmed by comparing the curves of weight-loss percentage, CMC enzyme activity, xylanase activity, as well as PCR-DGGE analysis of CSS-1’s different generations. The microbial composition of CSS-1 was analyzed by cloning library method. 【Result】 The average of cellulase activity and the maximum cellulase activity produced by CSS-1 were 79% and 82% higher respectively than those of the widely used inoculant A in corn stalk decomposition experiments. The weight-loss of the corn stalks treated with CSS-1 increased by 62.62% and 173.65 % compared with that of blank control and the inoculant A, respectively. The CSS-1 had high decomposition efficiency for corn stalks and microbial composition stability was confirmed by determining and evaluating the subculture of 22 generations. The results of cloning library method showed that the dominant bacteria of CSS-1 belonged to Enterobacter sp., Cellulomonas sp., Streptomyces sp., Bacillaceae sp., Pantoea sp. and Cellvibrio sp. and the dominant fungi were Trichoderma sp. and Gibberella sp. 【Conclusion】 The complex microbial system CSS-1 has high efficiency corn-stalk decaying ability under natural conditions, and its microbial component and decomposition function are suitable for corn stalks returned to fields in Northern China. |
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| Keywords: | corn stalk complex microbial system decomposition composition analysis cloning library |
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